Role of GPER on proliferation, migration and invasion in ligand‐independent manner in human ovarian cancer cell line SKOV3
G protein‐coupled estrogen receptor (GPER) is identified as a critical estrogen receptor, in addition to the classical estrogen receptors ERα and ERβ. In ERα‐negative ovarian cancer cells, our previous studies have found that estrogen stimulated cell proliferation and metastasis via GPER. However, the ligand‐independent function of GPER in ovarian cancer cells is still not clear. Herein, we describe that GPER has a co‐expression with ERα and ERβ, which are first determined in SKOV3 ovarian cancer cell line. In the absence of estrogen, GPER depletion by specific siRNA inhibits the proliferation, migration and i...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Yan Yan, Xueli Jiang, Ying Zhao, Haixia Wen, Guoyi Liu Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Tags: Issue Information Source Type: research
Association of DNA methyltransferases expression with global and gene‐specific DNA methylation in colorectal cancer cells
There are conflicting reports regarding the association between DNA methyltransferases (DNMTs) expression and global or gene‐specific DNA methylation in colorectal cancer (CRC) cells. To correlate DNMTs expression with DNA methylation, we quantified DNMT1, DNMT3A and DNMT3B mRNA levels in five CRC cell lines (HCT116, LS180, HT29/219, Caco2 and SW742) by real‐time reverse‐transcriptase polymerase chain reaction (PCR) assay. In addition, we examined the global 5‐methyl cytosine levels and the methylation patterns of 12 CpG islands in these CRC cells by enzyme‐linked immunosorbent assay and methylation‐specific PC...
Source: Cell Biochemistry and Function - September 28, 2015 Category: Biochemistry Authors: Mostafa Moradi Sarabi, Fakhraddin Naghibalhossaini Tags: Research Article Source Type: research
Hypothalamic energy metabolism is impaired by doxorubicin independently of inflammation in non‐tumour‐bearing rats
We sought to explore the effects of doxorubicin on inflammatory profiles and energy metabolism in the hypothalamus of rats. To investigate these effects, we formed two groups: a control (C) group and a Doxorubicin (DOXO) group. Sixteen rats were randomly assigned to either the control (C) or DOXO groups. The hypothalamus was collected. The levels of interleukin (IL)‐1β, IL‐6, IL‐10, TNF‐α and energy metabolism (malate dehydrogenase, complex I and III activities) were analysed in the hypothalamus. The DOXO group exhibited a decreased body weight (p < 0.01). Hypothalamic malate dehydrogenase activity was red...
Source: Cell Biochemistry and Function - September 16, 2015 Category: Biochemistry Authors: Barbara Moura Mello Antunes, Fabio Santos Lira, Gustavo Duarte Pimentel, José Cesar Rosa Neto, Andrea Maculano Esteves, Lila Missae Oyama, Cláudio Teodoro Souza, Cinara Ludvig Gonçalves, Emilio Luiz Streck, Bruno Rodrigues, Ronaldo Vagner dos Santos, M Tags: Research Article Source Type: research
Dynamin function is important for chemokine receptor‐induced cell migration
The HIV viral entry co‐receptors CCR5 and CXCR4 function physiologically as typical chemokine receptors. Activation leads to cytosolic signal transduction that results in a variety of cellular responses such as cytoskeletal rearrangement and chemotaxis (CTX). Our aim was to investigate the signalling pathways involved in CC and CXC receptor‐mediated cell migration. Inhibition of dynamin I and II GTPase with dynasore completely inhibited CCL3‐stimulated CTX in THP‐1 cells, whereas the dynasore analogue Dyngo‐4a, which is a more potent inhibitor, showed reduced ability to inhibit CC chemokine‐induced CTX. In cont...
Source: Cell Biochemistry and Function - September 9, 2015 Category: Biochemistry Authors: Richard O. Jacques, Shirley C. Mills, Paula Cazzonatto Zerwes, Feyisope O. Fagade, John E. Green, Scott Downham, Darren W. Sexton, Anja Mueller Tags: Research Article Source Type: research
PDZRN4 acts as a suppressor of cell proliferation in human liver cancer cell lines
Recently, some reports show that Ligand of Numb Protein‐X 1 (LNX1) could be a suppressor gene in gliomas, while our current research has firstly shown that PDZ domain containing ring finger 4 (PDZRN4), another member of LNX family, could also be a potential suppressor in hepatocellular carcinoma (HCC). PDZRN4, also named LNX4 (Ligand of Numb Protein‐X 4), is a member of the LNX family. We recently found that PDZRN4, but not LNX1, was down‐regulated in HCC samples, and the role of PDZRN4 in the progression of HCC had not been studied before. To address this question, firstly, we evaluated the expression of PDZRN4 in H...
Source: Cell Biochemistry and Function - August 1, 2015 Category: Biochemistry Authors: Taotao Hu, Hong Yang, Ze‐Guang Han Tags: Research Article Source Type: research
Role of exercise intensity on GLUT4 content, aerobic fitness and fasting plasma glucose in type 2 diabetic mice
Type 2 diabetes mellitus (T2D) results in several metabolic and cardiovascular dysfunctions, clinically characterized by hyperglycaemia due to lower glucose uptake and oxidation. Physical exercise is an effective intervention for glycaemic control. However, the effects of exercising at different intensities have not yet been addressed. The present study analysed the effects of 8 weeks of training performed at different exercise intensities on type 4 glucose transporters (GLUT4) content and glycaemic control of T2D (ob/ob) and non‐diabetic mice (ob/OB). The animals were divided into six groups, with four groups being su...
Source: Cell Biochemistry and Function - August 1, 2015 Category: Biochemistry Authors: Verusca Najara Cunha, Mérica Paula Lima, Daisy Motta‐Santos, Jorge Luiz Pesquero, Rosangela Vieira Andrade, Jeeser Alves Almeida, Ronaldo Carvalho Araujo, Carmen Silvia Grubert Campbell, John E. Lewis, Herbert Gustavo Simões Tags: Research Article Source Type: research
Mycophenolic acid reverses TGF beta‐induced cell motility, collagen matrix contraction and cell morphology in vitro
Abstract
The aim of this study was to elucidate functional and molecular effects of mycophenolic acid (MPA) on non‐lymphatic, kidney epithelial cells treated with transforming growth factor (TGF).
MPA effects were studied using HK2 cells incubated with EGF and TGF. The reversibility of these effects was verified using guanosine and 8‐aminoguanosine. The following assays were applied: cell proliferation, viability, collagen matrix contraction, scratch wound closure, spindle index, FACS with anti‐CD29 and anti‐CD326, promoter demethylation of RAS protein activator like 1 (RASAL1), as well as gene expression of RASAL1...
Source: Cell Biochemistry and Function - August 1, 2015 Category: Biochemistry Authors: Darinka Todorova Petrova, Gunnar Brandhorst, Christian Koch, Frank Christian Schultze, Christoph Eberle, Philip D. Walson, Michael Oellerich Tags: Research Article Source Type: research
Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1)
Heat shock protein 20 (HSP20) has cardioprotective qualities, which are triggered by PKA phosphorylation. PKD1 is also a binding partner for HSP20, and this prompted us to investigate whether the chaperone was a substrate for PKD1. We delineate the PKD1 binding sites on HSP20 and show for the first time HSP20 is a substrate for PKD1. Phosphorylation of HSP20 by PKD1 is diminished by pharmacological or siRNA reduction of PKD1 activity and is enhanced following PKD1 activation. Our results suggest that both PKA and PKD1 can both phosphorylate HSP20 on serine 16 but that PKA is the most dominant. Copyright © 2015 John Wiley ...
Source: Cell Biochemistry and Function - August 1, 2015 Category: Biochemistry Authors: Yuan Yan Sin, George S. Baillie Tags: Rapid Communication Source Type: research
Esculetin induces apoptosis in human colon cancer cells by inducing endoplasmic reticulum stress
Colorectal cancer has become more common in many regions of the world. Recently, we showed that esculetin, a natural coumarin, induces apoptosis in HT‐29 colon cancer cells via the reactive oxygen species‐mediated mitochondrial pathway. The present study examined whether esculetin induces apoptosis in HT‐29 colon cancer cells by inducing endoplasmic reticulum (ER) stress. We found that esculetin induced characteristic signs of ER stress, confirmed by ER staining, mitochondrial calcium overload and expression of ER stress‐related proteins (i.e. glucose regulated protein 78, phosphorylated ribonucleic acid‐dependen...
Source: Cell Biochemistry and Function - August 1, 2015 Category: Biochemistry Authors: Areum Daseul Kim, Susara Ruwan Kumara Madduma Hewage, Mei Jing Piao, Kyoung Ah Kang, Suk Ju Cho, Jin Won Hyun Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 24, 2015 Category: Biochemistry Tags: Issue Information Source Type: research
Proinflammatory interleukins' production by adipose tissue‐derived mesenchymal stromal cells: the impact of cell culture conditions and cell‐to‐cell interaction
The impact of culture conditions and interaction with activated peripheral blood mononuclear cells on the interleukin (IL) gene expression profile and proinflammatory IL‐6 and IL‐8 production by adipose‐derived stromal cells (ASCs) was investigated. A microarray analysis revealed a wide range of IL genes either under standard (20%) or hypoxic (5%) O2 concentrations, some highly up‐regulated at hypoxia. IL‐6 and IL‐8 production was inversely dependent on cell culture density. In early (first–third) passages, IL‐6 and IL‐8 concentration was higher at 20% O2 and in late (8th‐12th) passages under 5% O2. Int...
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Elena Andreeva, Irina Andrianova, Julia Rylova, Aleksandra Gornostaeva, Polina Bobyleva, Ludmila Buravkova Tags: Research Article Source Type: research
Inhibition of MCL‐1 by obatoclax sensitizes Sp2/0‐Ag14 hybridoma cells to glutamine deprivation‐induced apoptosis
In this report, we examined the role of the anti‐apoptotic proteins of the B‐cell lymphoma 2 (BCL‐2) protein family in the survival of Sp2/0‐Ag14 (Sp2/0) mouse hybridoma cells, a cell line that undergoes apoptosis within minutes of Gln deprivation. Western blot analysis revealed that myeloid cell leukaemia 1 (MCL‐1) was expressed at much higher levels than BCL‐2, B‐cell lymphoma extra‐large and BCL‐2‐like protein 2 making it the prominent pro‐survival BCL‐2 family member in this hybridoma. Gln deprivation triggered a progressive decrease in MCL‐1 protein levels, which coincided with the decrease i...
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Curtis C. Harnett, Abdelmuhsen Abusneina, Julie Clément, Eric R. Gauthier Tags: Research Article Source Type: research
LINE‐1 hypomethylation induced by reactive oxygen species is mediated via depletion of S‐adenosylmethionine
In conclusion, in bladder cancer and normal kidney cells exposed to H2O2, SAM and Hcy were decreased, but total glutathione was increased. Treatments with antioxidants (TA and NAC) and one‐carbon metabolites (SAM, methionine and folic acid) restored these changes. This pioneer finding suggests that exposure of cells to ROS activates glutathione synthesis via the transsulfuration pathway leading to deficiency of Hcy, which consequently causes SAM depletion and eventual hypomethylation of LINE‐1. Copyright © 2015 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Chiraphat Kloypan, Monpicha Srisa‐art, Apiwat Mutirangura, Chanchai Boonla Tags: Research Article Source Type: research
Knockdown of NF‐κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl‐2 apoptotic pathway
This study aimed to assess the effects of NF‐κB p65 subunit knockdown in suppression of nude mouse lung tumour cell xenografts and understands the underlying molecular events. A nude mouse Lewis lung carcinoma cell xenograft model was established, and the mice were intraperitoneally injected with NF‐κB p65 small interfering RNA (siRNA) and sacrificed after 2weeks of tumour cell injection. Tumour xenografts were harvested for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Western blot and quantitative reverse transcription‐polymerase chain reaction analyses. Results found that compared t...
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Yun Qu, Bo Qu, Xiaofang Wang, Rong Wu, Xiaoye Zhang Tags: Research Article Source Type: research
