The expression of tumor suppressor gene Cyld is upregulated by histone deacetylace inhibitors in human hepatocellular carcinoma cell lines
CYLD is a deubiquitinating enzyme that exerts a tumor suppressive function. Its downregulation or inactivation has been associated with the development of several types of malignancies including hepatocellular carcinoma (HCC). HCC cells display significantly lower Cyld expression compared to primary human hepatocytes, and Cyld downregulation can contribute to apoptotic resistance of HCC cells. Little is known about the mechanism of Cyld downregulation in human HCC cells. In the present study we explored the possible regulation of Cyld expression by histone deacetylases (HDACs) in human HCC cell lines. We demonstrated that ...
Source: Cell Biochemistry and Function - July 31, 2016 Category: Biochemistry Authors: Panorea Kotantaki, George Mosialos Tags: RAPID COMMUNICATION Source Type: research
Preventive effect of Desferal on sperm motility and morphology
Transition metal ions, mainly iron, are involved in the generation of highly reactive hydroxyl radicals, which are the most powerful inducers of oxidative damage to all biomolecules. The lipids in sperm membranes are highly susceptible to oxidation. Sperm lipid peroxidation (LPO) leads to decrease of motility and reduction of likelihood for sperm‐oocyte fusion. The excess radical production may affect also the spermatozoa morphology. The aim of the present study was to investigate the effect of Desferal on the LPO, motility, and morphology of boar sperm subjected to oxidative stress. After collection, the ejaculates were...
Source: Cell Biochemistry and Function - July 28, 2016 Category: Biochemistry Authors: Galina Nenkova, Rossen Stefanov, Mihail Chervenkov, Albena Alexandrova Tags: RESEARCH ARTICLE Source Type: research
Lactate Formation in Primary and Metastatic Colon Cancer Cells at Hypoxia and Normoxia
High glucose consumption and lactate synthesis in aerobic glycolysis are a hallmark of cancer cells. They can form lactate also in glutaminolysis, but it is not clear how oxygen availability affects this process. We studied lactate synthesis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured with L‐Ser and/or L‐Asp. Glucose and lactate levels were determined colorimetrically, amino acids by HPLC, expression of AST1‐mRNA and AST2‐mRNA by RT‐PCR.
In both lines glucose consumption and lactate synthesis were higher at 10% than at 1% oxygen, and lactate/glucose ratio w...
Source: Cell Biochemistry and Function - June 30, 2016 Category: Biochemistry Authors: Wojciech Grabo ń, Dagmara Otto‐Ślusarczyk, Alicja Chrzanowska, Magdalena Mielczarek‐Puta, Ilona Joniec‐Maciejak, Krzysztof Słabik, Anna Barańczyk‐Kuźma Tags: RESEARCH ARTICLE Source Type: research
Allergen ‐specific immune response suppresses interleukin 10 expression in B cells via increasing micro‐RNA‐17‐92 cluster
In conclusion, miR‐19a mediates the allergen‐specific immune response–decreased IL‐10 expression in B cells. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 30, 2016 Category: Biochemistry Authors: Xiao ‐Rui Geng, Shu‐Qi Qiu, Li‐Tao Yang, Zhi‐Qiang Liu, Gui Yang, Jiang‐Qi Liu, Lu Zeng, Xiao‐Xi Li, Li‐Hua Mo, Zhi‐Gang Liu, Ping‐Chang Yang Tags: RESEARCH ARTICLE Source Type: research
Bisphenol A regulates Snail ‐mediated epithelial‐mesenchymal transition in hemangioma cells
Hemangioma (HA) can be exposed to bisphenol A (BPA) through direct skin absorption. Although numerous studies indicated that BPA can trigger the progression of cancers, there is no study concerning the effects of BPA on development of HA. Our present study revealed that nanomolar BPA can significantly increase the in vitro migration and invasion of HA cells via induction of epithelial‐mesenchymal transition (EMT), which was evidenced by the upregulation of vimentin and downregulation of E‐cadherin. The BPA treatment also significantly increased the expression and nuclear localization of Snail and the key transcription ...
Source: Cell Biochemistry and Function - June 30, 2016 Category: Biochemistry Authors: Denggao Zhai, Jiantai He, Xiaoli Li, Liansheng Gong, Yang Ouyang Tags: RESEARCH ARTICLE Source Type: research
Abnormal photoreceptor outer segment development and early retinal degeneration in kif3a mutant zebrafish
Photoreceptors are highly specialized sensory neurons that possess a modified primary cilium called the outer segment. Photoreceptor outer segment formation and maintenance require highly active protein transport via a process known as intraflagellar transport. Anterograde transport in outer segments is powered by the heterotrimeric kinesin II and coordinated by intraflagellar transport proteins. Here, we describe a new zebrafish model carrying a nonsense mutation in the kinesin II family member 3A (kif3a) gene. Kif3a mutant zebrafish exhibited curved body axes and kidney cysts. Outer segments were not formed in most parts...
Source: Cell Biochemistry and Function - June 30, 2016 Category: Biochemistry Authors: Rakesh K. Raghupathy, Xun Zhang, Reem H. Alhasani, Xinzhi Zhou, Margaret Mullin, James Reilly, Wenchang Li, Mugen Liu, Xinhua Shu Tags: RESEARCH ARTICLE Source Type: research
Understanding perspectives of signalling mechanisms regulating PEBP1 function
Phosphatidylethanolamine‐binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein, belongs to PEBP family of proteins. It is known to interact with many proteins that are mainly involved in pathways that monitor cell proliferation and differentiation. PEBP1 in many cells interacts with several pathways, namely MAPK, GRK2, NF‐кB, etc. that keeps the cell proliferation and differentiation in check. This protein is expressed by many cells in humans, including neurons where it is predominantly involved in production of choline acetyltransferase. Deregulated PEBP1 is known to cause cancer, diabetic nephropathy...
Source: Cell Biochemistry and Function - June 30, 2016 Category: Biochemistry Authors: Karthik Rajkumar, Aare Nichita, Pawan Kumar Anoor, Swathi Raju, Surya Satyanarayana Singh, Sandeepta Burgula Tags: Review Article Source Type: research
Down‐expression of miR‐154 suppresses tumourigenesis in CD133+ glioblastoma stem cells
Glioblastoma multiforme (GBM) is the most common and aggressive form of brain cancer. Evidences have suggested that CD133 is a marker for a subset of glioblastoma cancer stem cells. However, whether miRNA plays a critical role in CD133+ GBM is poorly understood. Here, we identified that miR‐154 was upregulated in CD133+ GBM cell lines. Knockdown of miR‐154 remarkably suppressed proliferation and migration of CD133+ GBM cells. Further study found that PRPS1 was a direct target of miR‐154 in CD133+ GBM cells. Overexpression of PRPS1 exhibited similar effects as miR‐154 knockdown in CD133+ GBMs. Our study identified m...
Source: Cell Biochemistry and Function - June 24, 2016 Category: Biochemistry Authors: Liang Yang, Zhongjie Yan, Yuanyu Wang, Wandong Ma, Chen Li Tags: Research Article Source Type: research
Down ‐expression of miR‐154 suppresses tumourigenesis in CD133+ glioblastoma stem cells
Glioblastoma multiforme (GBM) is the most common and aggressive form of brain cancer. Evidences have suggested that CD133 is a marker for a subset of glioblastoma cancer stem cells. However, whether miRNA plays a critical role in CD133+ GBM is poorly understood. Here, we identified that miR‐154 was upregulated in CD133+ GBM cell lines. Knockdown of miR‐154 remarkably suppressed proliferation and migration of CD133+ GBM cells. Further study found that PRPS1 was a direct target of miR‐154 in CD133+ GBM cells. Overexpression of PRPS1 exhibited similar effects as miR‐154 knockdown in CD133+ GBMs. Our study identified m...
Source: Cell Biochemistry and Function - June 23, 2016 Category: Biochemistry Authors: Liang Yang, Zhongjie Yan, Yuanyu Wang, Wandong Ma, Chen Li Tags: Research Article Source Type: research
Ouabain ‐induced changes in MAP kinase phosphorylation in primary culture of rat cerebellar cells
In this study we investigated the effect of ouabain on the level of phosphorylation of three MAPK (ERK1/2, JNK and p38) and on cell survival in the primary culture of rat cerebellar cells. Using Western blotting we described the time course and concentration dependence of phosphorylation for ERK1/2, JNK and p38 in response to ouabain. We discovered that ouabain at a concentration of 1 μM does not cause cell death in cultured neurons while it changes the phosphorylation level of the three MAPK: ERK1/2 is phosphorylated transiently, p38 shows sustained phosphorylation, and JNK is dephosphorylated after a long‐term incub...
Source: Cell Biochemistry and Function - June 22, 2016 Category: Biochemistry Authors: Alexander V. Lopachev, Olga M. Lopacheva, Ekaterina A. Osipova, Elizaveta A. Vladychenskaya, Larisa V. Smolyaninova, Tatiana N. Fedorova, Olga V. Koroleva, Evgeny E. Akkuratov Tags: Research Article Source Type: research
Ouabain‐induced changes in MAP kinase phosphorylation in primary culture of rat cerebellar cells
In this study we investigated the effect of ouabain on the level of phosphorylation of three MAPK (ERK1/2, JNK and p38) and on cell survival in the primary culture of rat cerebellar cells. Using Western blotting we described the time course and concentration dependence of phosphorylation for ERK1/2, JNK and p38 in response to ouabain. We discovered that ouabain at a concentration of 1 μM does not cause cell death in cultured neurons while it changes the phosphorylation level of the three MAPK: ERK1/2 is phosphorylated transiently, p38 shows sustained phosphorylation, and JNK is dephosphorylated after a long‐term incub...
Source: Cell Biochemistry and Function - June 22, 2016 Category: Biochemistry Authors: Alexander V. Lopachev, Olga M. Lopacheva, Ekaterina A. Osipova, Elizaveta A. Vladychenskaya, Larisa V. Smolyaninova, Tatiana N. Fedorova, Olga V. Koroleva, Evgeny E. Akkuratov Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 22, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Down ‐regulation of semaphorin 3F in rat retinal ganglion cells in response to optic nerve crush
Glaucoma is characterized by degeneration of optic nerve axons and death of retinal ganglion cells (RGCs). Nerve crush and axotomy of the optic nerve are studied as models of RGC death in glaucoma and of axon regeneration. The mechanisms underlying the response of RGCs to axonal injury remain unclear, however. We have now examined the effects of optic nerve crush on the expression of members of the semaphorin family of neuronal guidance proteins in the rat retina. The expression of semaphorin 3F (Sema3F) in the retina was down‐regulated at both the mRNA and protein levels at 7 days after optic nerve injury, whereas tha...
Source: Cell Biochemistry and Function - June 15, 2016 Category: Biochemistry Authors: Ji ‐Ae Ko, Akira Minamoto, Yosuke Sugimoto, Yoshiaki Kiuchi Tags: Research Article Source Type: research
Inhibition of autophagy and enhancement of endoplasmic reticulum stress increase sensitivity of osteosarcoma Saos‐2 cells to cannabinoid receptor agonist WIN55,212‐2
In this study, we examined the effect of WIN55,212‐2 on osteosarcoma cell line Saos‐2 in terms of cell viability and apoptosis. Meanwhile, we further explored the role of endoplasmic reticulum stress and autophagy in apoptosis induced by WIN55,212‐2. Our results showed that the cell proliferation of Saos‐2 was inhibited by WIN55,212‐2 in a dose‐dependent and time‐dependent manner. WIN55,212‐2‐induced Saos‐2 apoptosis through mitochondrial apoptosis pathway. Meanwhile, WIN55,212‐2 can induce endoplasmic reticulum stress and autophagy in Saos‐2 cells. Inhibition of autophagy and enhancement of endopla...
Source: Cell Biochemistry and Function - June 15, 2016 Category: Biochemistry Authors: Guodong Zhang, Haiyong Bi, Ji Gao, Xing Lu, Yanping Zheng Tags: Research Article Source Type: research
Ceranib ‐2‐induced suicidal erythrocyte death
Ceramide is known to trigger apoptosis of nucleated cells and eryptosis of erythrocytes. Eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Besides ceramide, stimulators of eryptosis include increase of cytosolic Ca2+‐activity ([Ca2+]i) and oxidative stress. Ceramide is degraded by acid ceramidase and inhibition of the enzyme similarly triggers apoptosis. The present study explored, whether ceramidase inhibitor Ceranib‐2 induces eryptosis. Flow cytometry was employed to quantify phosphatidylserine‐exposure at the cell surface fro...
Source: Cell Biochemistry and Function - June 11, 2016 Category: Biochemistry Authors: Elena Signoretto, Jens Zierle, Abdulla Al Mamun Bhuyan, Michela Castagna, Florian Lang Tags: Research Article Source Type: research
