Mesenchymal stromal cells ameliorate acute allergic rhinitis in rats
Mesenchymal stromal cells (MSCs) have been extensively investigated as a potential antiinflammatory treatment in many inflammatory‐related diseases; however, it remains unclear whether MSCs could be used to treat acute allergic rhinitis. A rat model of allergic rhinitis was treated with MSCs. The effect of MSCs on the inflammation of allergic rhinitis was evaluated by sneezing, nose rubbing, the pathology of the nasal mucosa, and the expression of interleukin 4, tumour necrosis factor alpha, and immunoglobulin E in the serum of rats. Also, the population of MSCs isolated from umbilical cords of humans was evaluated to de...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Chunlei Li, Yanxia Fu, Yinyin Wang, Yanhua Kong, Mengdi Li, Danhui Ma, Wanli Zhai, Hao Wang, Yuting Lin, Sihan Liu, Fangli Ren, Jun Li, Yi Wang Tags: RESEARCH ARTICLE Source Type: research
Role of macrophage migration inhibitory factor (MIF) in the effects of oxidative stress on human retinal pigment epithelial cells
Proliferative vitreoretinopathy (PVR) is the major cause of treatment failure in individuals who undergo surgery for retinal detachment. The epithelial‐mesenchymal transition (EMT) in retinal pigment epithelium (RPE) cells contributes to the pathogenesis of PVR. Oxidative stress is thought to play a role in the progression of retinal diseases including PVR. We have now examined the effects of oxidative stress on the EMT and related processes in the human RPE cell line. We found that H2O2 induced the contraction of RPE cells in a three‐dimensional collagen gel. Analysis of a cytokine array revealed that H2O2 specificall...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Ji ‐Ae Ko, Yasuyuki Sotani, Diah Gemala Ibrahim, Yoshiaki Kiuchi Tags: RESEARCH ARTICLE Source Type: research
Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation
Induced pluripotent stem (iPS) cells are important for clinical application and stem cell research. Although human melanoma‐associated antigen A2 (hMAGEA2) expression is known to affect differentiation in embryonic stem cells, its specific role in iPS cells remains unclear. To evaluate the function of hMAGEA2 and its characteristics in iPS cells, we produced hMAGEA2‐overexpressing iPS cells from hMAGEA2‐overexpressing transgenic mice. Although the iPS cells with overexpressed hMAGEA2 did not differ in morphology, their pluripotency, and self‐renewal related genes (Nanog, Oct3/4, Sox2, and Stat3), expression level w...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Song Park, Yonghun Sung, Jain Jeong, Minjee Choi, Jinhee Lee, Wookbong Kwon, Soyoung Jang, Si Jun Park, Jae Young Kim, Sung Hyun Kim, Duhak Yoon, Zae Young Ryoo, Myoung Ok Kim Tags: RESEARCH ARTICLE Source Type: research
Activation of the mitochondrial apoptotic pathway contributes to methotrexate ‐induced small intestinal injury in rats
The efficacy of methotrexate (MTX), a commonly used chemotherapeutic drug, is limited by intestinal injury. As the mechanism of MTX‐induced small intestinal injury is not clear, there is no definitive treatment for MTX‐induced gastrointestinal injury. The present study investigates the role of mitochondrial apoptotic pathway in MTX‐induced small intestinal injury and examines whether aminoguanidine is effective in preventing the damage. Eight Wistar rats were administered 3 consecutive i.p. injections of 7 mg/kg body wt. MTX. Some rats were pretreated with 30 mg or 50 mg/kg body wt. of aminoguanidine (n = 6 in e...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Kasthuri Natarajan, Premila Abraham, Rekha Kota Tags: RESEARCH ARTICLE Source Type: research
Characterization of ectonucleoside triphosphate diphosphohydrolase (E ‐NTPDase; EC 3.6.1.5) activity in mouse peritoneal cavity cells
This study aimed to characterize the activity of ectonucleoside triphosphate diphosphohydrolase (E‐NTPDase; EC 3.6.1.5) in peritoneal cavity cells from BALB/c mice. E‐NTPDase was activated in the presence of both calcium (1.5mM) and magnesium (1.5mM) ions. However, the activity was higher in the presence of Ca2+. A pH of 8.5 and temperature of 37°C were the optimum conditions for catalysis. The apparent Km values were 0.51mM and 0.66mM for the hydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP), respectively. The Vmax values were 136.4 and 120.8 nmol Pi/min/mg of protein for ATPase and ADPase a...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Dh ébora Albuquerque Dias, Bruna Penteado, Lucas Derbocio Santos, Pedro Mendes Santos, Carla Cardozo Pinto Arruda, Maria Rosa Chitolina Schetinger, Daniela Bitencourt Rosa Leal, Jeandre Augusto Jaques Tags: RESEARCH ARTICLE Source Type: research
LRF inhibits p53 expression in colon cancer cells via modulating DAP5 activity
In conclusion, PAR2 activation increases the expression of LRF in colon cancer cells, which interacts with DAP5 to repress the p53 expression. Leukaemia/lymphoma‐related factor may be a novel target in the treatment of colon cancer. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Min Zhu, Peng Wang, Fan Feng, Ming ‐Yang Li Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Recuperating lung decoction attenuates inflammation and oxidation in cigarette smoke ‐induced COPD in rats via activation of ERK and Nrf2 pathways
This study evaluated the effect of recuperating lung decoction (RLD) on inflammation and oxidative stress in rats with COPD induced by cigarette smoke and lipopolysaccharides (LPS). We used intravenous infusion of LPS combined with cigarette smoke exposure as a COPD rat model. We observed that RLD treatment increased the protein level of GSH and the ratio of GSH/GSSG but decreased 8‐OHdG and 4‐HNE in the serum. Furthermore, RLD significantly inhibited the expressions of IL‐1β, IL‐6, TNF‐α, and TGF‐β induced by cigarette smoke exposure, reduced the number of inflammatory cells in the bronchoalveolar lavage fl...
Source: Cell Biochemistry and Function - July 27, 2017 Category: Biochemistry Authors: Chunlei Li, Yue Yan, Qi Shi, Yanhua Kong, Longxia Gao, Haipeng Bao, Youlin Li Tags: RESEARCH ARTICLE Source Type: research
Cellular and genetic models of H6PDH and 11 β‐HSD1 function in skeletal muscle
Glucocorticoids are important for skeletal muscle energy metabolism, regulating glucose utilization, insulin sensitivity, and muscle mass. Nicotinamide adenine dinucleotide phosphate‐dependent 11β‐hydroxysteroid dehydrogenase type 1 (11β‐HSD1)‐mediated glucocorticoid activation in the sarcoplasmic reticulum (SR) is integral to mediating the detrimental effects of glucocorticoid excess in muscle. 11β‐Hydroxysteroid dehydrogenase type 1 activity requires glucose‐6‐phosphate transporter (G6PT)‐mediated G6P transport into the SR for its metabolism by hexose‐6‐phosphate dehydrogenase (H6PDH) for NADPH gen...
Source: Cell Biochemistry and Function - July 27, 2017 Category: Biochemistry Authors: Agnieszka E. Zielinska, Rachel S. Fletcher, Mark Sherlock, Craig L. Doig, Gareth G. Lavery Tags: RESEARCH ARTICLE Source Type: research
MiR ‐429 suppresses glioblastoma multiforme by targeting SOX2
Accumulating evidence has shown that miR‐429 plays an important role in the development and progression of tumour. However, the role of miR‐429 in glioblastoma multiforme (GBM) remains largely unknown. The present study is designed to investigate the function of miR‐429 in GBM and to explore the molecular mechanism underlying its function. The expression level of miR‐429 was detected in GBM tissues and cell lines by quantitative real‐time polymerase chain reaction. The effect of overexpression of miR‐429 on in vitro cell proliferation, apoptosis and invasion was examined. Western blot analysis was used to detec...
Source: Cell Biochemistry and Function - July 27, 2017 Category: Biochemistry Authors: Huixiao Dong, Xiuzhen Hao, Benliang Cui, Meiling Guo Tags: RESEARCH ARTICLE Source Type: research
Vitamin D insufficiency correlates with peripheral B10 cells in patients with pituitary tumours
In conclusion, modulation of the expression of VDR can regulate the expression of IL‐10 in peripheral B cells of PGT patients, which may contribute to the treatment of PGT. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 27, 2017 Category: Biochemistry Authors: Weimin Wang, Li Fu, Shengli Li, Zhiming Xu, Peng Qiu, Tong ‐Jiang Xu, Wei Yang, Yu‐Bao Zhang, Guang‐Ming Xu, Xiang‐Dong Lu, Xinggang Li Tags: RESEARCH ARTICLE Source Type: research
Effects of propolis, caffeic acid phenethyl ester, and pollen on renal injury in hypertensive rat: An experimental and theoretical approach
In conclusion, experimental and theoretical data suggested that oxidative alterations occurring in the kidney tissue of chronic hypertensive rats may be prevented via active compound of propolis, CAPE administration. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 1, 2017 Category: Biochemistry Authors: Ramin Ekhteiari Salmas, Mehmet Fuat Gulhan, Serdar Durdagi, Engin Sahna, Huda I. Abdullah, Zeliha Selamoglu Tags: RESEARCH ARTICLE Source Type: research
Exosomal DNMT1 mediates cisplatin resistance in ovarian cancer
Ovarian cancer is the most common malignancy in women. Owing to late syndromic presentation and lack of efficient early detection, most cases are diagnosed at advanced stages. Surgery and platinum‐based chemotherapy are still the standard care currently. However, resistance invoked often compromises the clinical value of the latter. Expression of DNA methyltransferase 1 (DNMT1) was analysed by gene array. Protein was determined by immunoblotting. Exosome was isolated with commercial kit. Cell proliferation was measured by CCK8 method. Annexin V‐PI double staining was performed for apoptosis evaluation. Xenograft model ...
Source: Cell Biochemistry and Function - July 1, 2017 Category: Biochemistry Authors: Ya ‐Lei Cao, Ting Zhuang, Bao‐Heng Xing, Na Li, Qin Li Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 1, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Corrigendum: Strain uses gap junctions to reverse stimulation of osteoblast proliferation by osteocytes
(Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 6, 2017 Category: Biochemistry Authors: Rosemary F.L. Suswillo, Behzad Javaheri, Simon C.F. Rawlinson, Gary P. Dowthwaite, Lance E. Lanyon, Andrew A. Pitsillides Tags: CORRIGENDUM Source Type: research
