Quantitative proteomics reveals that miR ‐222 inhibits erythroid differentiation by targeting BLVRA and CRKL
miR‐222 plays an important role in erythroid differentiation, but the potential targets of miR‐222 in the regulation of erythroid differentiation remain to be determined. The target genes of miR‐222 were identified by proteomics combined with bioinformatics analysis in this study. Thirteen proteins were upregulated, and 13 were downregulated in K562 cells following transfection with miR‐222 inhibitor for 24 and 48 hours. Among these proteins, BLVRA and CRKL were upregulated after transfection of miR‐222 inhibitor in K562 cells and human CD34+ HPCs. Moreover, miR‐222 mimics reduced and miR‐222 inhibitor enhan...
Source: Cell Biochemistry and Function - January 1, 2018 Category: Biochemistry Authors: Li Jiang, Xing Wang, Yong Wang, Xiaoyan Chen Tags: RESEARCH ARTICLE Source Type: research
ILEI is an important intermediate participating in the formation of TGF ‐β1–induced renal tubular EMT
In conclusion, ILEI appears to play a crucial role in mediating TGF‐β1–induced EMT through the Akt and ERK pathways, which may provide a therapeutic target for the treatment of fibrotic kidney diseases.
Significance of the studyThere is no study reporting the effect of ILEI in renal EMTs. In this research, we examined the role and mechanism of ILEI in EMT using tubular epithelial cell; we found that ILEI participated in renal tubular EMT, and overexpression of ILEI can not only induce EMT of HK‐2 cells independently but also enhance EMT in response to TGF‐β1. Meanwhile, we found ILEI small interfering RNA blocked...
Source: Cell Biochemistry and Function - January 1, 2018 Category: Biochemistry Authors: Xing Zhao, Gang Luo, Yan Fan, Xiaoxue Ma, Jieqing Zhou, Hong Jiang Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - January 1, 2018 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
The influence of Shc proteins and high ‐fat diet on energy metabolism of mice
We report higher EE in ShcKO versus WT mice when consuming the HFD. Although decreased levels of Shc proteins influenced the activity of a central enzyme of β‐oxidation in response to high‐fat feeding, it did not produce concerted changes in enzymes of glycolysis, citric acid cycle, or the ETC. Thus, an increase in EE in response to consumption of an HFD may be a mechanism that leads to decreased weight gain previously reported in ShcKO mice with long‐term consumption of an HFD. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 14, 2017 Category: Biochemistry Authors: W.A. Baldassini, J.J. Ramsey, K. Hagopian, D.P.D. Lanna Tags: RESEARCH ARTICLE Source Type: research
LncRNA PCAT ‐1 promotes tumour growth and chemoresistance of oesophageal cancer to cisplatin
Oesophageal cancer (OC) is one of the most fatal malignancies in the world, and chemoresistance restricts the therapeutic outcome of OC. Long noncoding RNA (lncRNA) was reported to play roles in multiple cancer types. Yet, the function of lncRNA in chemoresistance of OC has not been reported. A lncRNA gene, PCAT‐1, showed higher expression in OC tissues, especially higher in secondary OC compared with normal mucosa tissues. Overexpression of PCAT‐1 increased the proliferation rate and growth of OC cells. Inhibition of PCAT‐1 decreased proliferation and growth of OC cells, and increased cisplatin chemosensitivity. In ...
Source: Cell Biochemistry and Function - December 1, 2017 Category: Biochemistry Authors: Qiang Zhen, Li ‐na Gao, Ren‐feng Wang, Wei‐wei Chu, Ya‐xiao Zhang, Xiao‐jian Zhao, Bao‐lei Lv, Jia‐bao Liu Tags: RESEARCH ARTICLE Source Type: research
Compared proteomic analysis of 8 ‐ and 32‐week‐old postnatal porcine ovaries
In this study, we used tandem mass tag quantitative proteomics to describe, for the first time, protein expression patterns of postnatal pig ovaries. Proteins involved in hormone metabolic pathways and maintenance, proliferation, and regulation of stem cells were identified. With further analysis by Interactive Pathway Explorer, proteins enriched in metabolism of steroid hormones, metabolism of lipids, and energy metabolism pathway were upregulated in the 32‐week‐old group, indicating physiological characteristics of sexual maturity. These findings have implications in applications of biomedicine. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 1, 2017 Category: Biochemistry Authors: Lin Hou, Jian Wang, Yinjuan Wang, Xiuguo Hua, Ji Wu Tags: RESEARCH ARTICLE Source Type: research
Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC ‐Ca2+‐CREB pathway
In conclusion, these data suggest that the effects of DES on mouse gubernaculum testis cells are mediated by PLC‐Ca2+‐CREB pathway.
Significance of the studyEnvironmental estrogens remain a serious threat to male reproductive health, and it is important to understand the mechanism by which EEs affect the male productive system. Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol. Our findings provide the first evidence that PLC‐Ca2+‐CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum t...
Source: Cell Biochemistry and Function - December 1, 2017 Category: Biochemistry Authors: Xuan Zhang, Hong ‐yan Ping, Jian‐hong Li, Shou‐xin Duan, Xue‐wu Jiang Tags: RESEARCH ARTICLE Source Type: research
Actions of p ‐synephrine on hepatic enzyme activities linked to carbohydrate metabolism and ATP levels in vivo and in the perfused rat liver
p‐Synephrine is one of the main active components of the fruit of Citrus aurantium (bitter orange). Extracts of the bitter orange and other preparations containing p‐synephrine have been used worldwide to promote weight loss and for sports performance. The purpose of the study was to measure the action of p‐synephrine on hepatic enzyme activities linked to carbohydrate and energy metabolism and the levels of adenine mononucleotides. Enzymes and adenine mononucleotides were measured in the isolated perfused rat liver and in vivo after oral administration of the drug (50 and 300 mg/kg) by using standard techniques. p...
Source: Cell Biochemistry and Function - December 1, 2017 Category: Biochemistry Authors: Marcos Rodrigues Maldonado, L ívia Bracht, Anacharis Babeto Sá‐Nakanishi, Rúbia Carvalho Gomes Corrêa, Jurandir Fernando Comar, Rosane Marina Peralta, Adelar Bracht Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 1, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Down ‐regulation of p16 and MGMT promotes the anti‐proliferative and pro‐apoptotic effects of 5‐Aza‐dC and radiation on cervical cancer cells
In this study, we stimulated cervical cancer cells with 5‐aza‐2′‐deoxycytidine (5‐Aza‐dC) and found that this treatment inhibited cell proliferation and induced apoptosis; additionally, methylation of p16 and O‐6‐methylguanine‐DNA methyltransferase (MGMT) was reversed, although their expression was suppressed. 5‐Aza‐dC inhibited E6 and E7 expression and up‐regulated p53, p21, and Rb expression. Cells transfected with siRNAs targeting p16 and MGMT as well as cells stimulated with 5‐Aza‐dC were arrested in S phase, and the expression of p53, p21, and Rb was up‐regulated more significantly. Howev...
Source: Cell Biochemistry and Function - November 15, 2017 Category: Biochemistry Authors: Guan ‐di Chen, De‐ying Qian, Zhi‐gang Li, Ge‐ying Fan, Ke‐li You, Yi‐long Wu Tags: RESEARCH ARTICLE Source Type: research
The involvement of histone methylation in osteoblastic differentiation of human periosteum ‐derived cells cultured in vitro under hypoxic conditions
This study was to examine the role hypoxia on osteoblastic differentiation of hPDCs, and we examined whether histone methylation is involved in the observed effect of hypoxia on osteogenic differentiation of hPDCs. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 30, 2017 Category: Biochemistry Authors: Dae ‐Kwan Yoon, Ji‐Sung Park, Gyu‐Jin Rho, Hyeon‐Jeong Lee, Iel‐Yong Sung, Jang‐Ho Son, Bong‐Wook Park, Young‐Hoon Kang, Sung‐Hoon Byun, Sun‐Chul Hwang, Dong Kyun Woo, Yeong‐Cheol Cho, June‐Ho Byun Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 30, 2017 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
miR ‐214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells
High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study. While the expression of miR‐214 remains suppressed, HMGA1 behaves as a potent oncogene and plays crucial roles in several aberrant signalling pathways by interacting with intermediates like RELA, CTNNB1, STAT3, and TP53 in CaCx and CRC. Hypothetically, miR‐214 should be able to regulate the stabilization of some of these intermediates through the regulation of HMGA1. This was assessed by ectopically expressing miR‐214 or complementarily, by inhibiting the express...
Source: Cell Biochemistry and Function - October 10, 2017 Category: Biochemistry Authors: Karthik Subramanian Chandrasekaran, Anusha Sathyanarayanan, Devarajan Karunagaran Tags: RESEARCH ARTICLE Source Type: research
Screening active components of modified Xiaoyao powder as NRF2 agonists
In conclusion, NRF2‐NQO1 pathway plays an essential role in mediating the activity of MXP and its active components, at least in part; some beneficial effects of MXP may be applicable to breast cancer chemoprevention. Our study firstly found MXP active components including quercetin, kaempferol, and atractylenolide II. Our results firstly demonstrate that NRF2‐NQO1 pathway plays an essential role in mediating the activity of MXP and its active components in breast cancer chemoprevention. Our study firstly found that atractylenolide II is a novel NRF2 inducer. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 1, 2017 Category: Biochemistry Authors: Yujie Yang, Yanran Wang, Ting Wang, Xuehua Jiang, Ling Wang Tags: RESEARCH ARTICLE Source Type: research
Lin28a enhances in vitro osteoblastic differentiation of human periosteum ‐derived cells
This study was to examine the impact of Lin28a on osteogenic phenotypes of human periosteum‐derived cells. Their phenotypes can be similar to those of mesenchymal stem cells. Our results suggest that the Lin28a gene enhances the osteoblastic differentiation of human periosteum‐derived cells. In addition, the Lin28a gene increases mitochondrial activity in human periosteum‐derived cells. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 1, 2017 Category: Biochemistry Authors: Jin ‐Ho Park, Bong‐Wook Park, Young‐Hoon Kang, Sung‐Hoon Byun, Sun‐Chul Hwang, Deok Ryong Kim, Dong Kyun Woo, June‐Ho Byun Tags: RESEARCH ARTICLE Source Type: research
