Bag‐1 promotes cell survival through c‐Myc‐mediated ODC upregulation that is not preferred under apoptotic stimuli in MCF‐7 cells
Bag‐1, Bcl‐2 associated athanogene‐1, is a multifunctional protein that can regulate a wide variety of cellular processes: proliferation, cell survival, transcription, apoptosis and motility. Bag‐1 interacts with various targets in the modulation of these pathways; yet molecular details of Bag‐1's involvement in each cellular event are still unclear. We first showed that forced Bag‐1 expression promotes cell survival and prevents drug‐induced apoptosis in MCF‐7 breast cancer cells. Increased mRNA expressions of c‐myc protooncogene and ornithine decarboxylase (ODC), biosynthetic enzyme of polyamines, were ...
Source: Cell Biochemistry and Function - July 15, 2015 Category: Biochemistry Authors: Pelin Ozfiliz, Tugba Kizilboga, Salih Demir, Gizem Alkurt, Narçin Palavan‐Unsal, Elif Damla Arisan, Gizem Dinler‐Doganay Tags: Research Article Source Type: research
Terrein reduces age‐related inflammation induced by oxidative stress through Nrf2/ERK1/2/HO‐1 signalling in aged HDF cells
This study investigated whether multiple bioactivity of terrein such as anti‐inflammatory and anti‐oxidant inhibits age‐related inflammation by promoting an antioxidant response in aged human diploid fibroblast (HDF) cells. HDF cells were cultured serially for in vitro replicative senescence. To create the ageing cell phenotype, intermediate stage (PD31) HDF cells were brought to stress‐induced premature senescence (SIPS) using hydrogen peroxide (H2O2). Terrein increased cell viability even with H2O2 stress and reduced inflammatory molecules such as intracellular adhesion molecule‐1 (ICAM‐1), cyclooxygenase‐2...
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Young‐Hee Lee, Sook‐Jeong Lee, Ji‐Eun Jung, Jeong‐Seok Kim, Nan‐Hee Lee, Ho‐Keun Yi Tags: Research Article Source Type: research
The use of erythrocyte fragility to assess xenobiotic cytotoxicity
The erythrocytes of mammals represent a good model to evaluate the cytotoxicity of molecules, organic and inorganic, natural or synthetic, by cellular damage measure. Indeed, before any investigation on the mechanism of action of different molecules, it is important to perform a cytotoxicity assay. Among the different cytotoxicity assays that assess a possible toxicity in the red blood cells is the rate of haemolysis. This essay is based on the evaluation of the alterations of red cell membranes in the presence of an eventual xenobiotic. Red blood cells are the main cells in circulation, and they are responsible for transp...
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Maria Pagano, Caterina Faggio Tags: Review Article Source Type: research
Genome‐wide screen of promoter methylation analysis of ES cells and ES derived epidermal‐like cells
In this study, we aimed to investigate differentially methylated promoters involved in process of differentiation from ESCs into epidermal‐like cells (ELCs) induced by human amnion. We successfully induced ESCs into ELCs, which expressed the surface markers of CK19, CK15 and β1‐integrin. With MeDIP‐chip arrays, we identified 3435 gene promoters to be differentially methylated, involving 894 HCP (high CpG‐containing promoter), 974 ICP (intermediate CpG‐containing promoter) and 1567 LCP (low CpG‐containing promoter) among all the 17 500 DNA methylation regions of gene promoters in both ESCs and ELCs. Gene onco...
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Ren‐li Zhang, Jin‐xiu Meng, Cai‐xia Liu, Li‐li Zhang, Dong Han, Jia‐jie Cai, An‐min Wen Tags: Research Article Source Type: research
ODAM inhibits RhoA‐dependent invasion in breast cancer
Odontogenic ameloblast‐associated protein (ODAM) contributes to cell adhesion. In human cancer, ODAM is down‐regulated, and the overexpression of ODAM results in a favourable prognosis; however, the molecular mechanisms underlying ODAM‐mediated inhibition of cancer invasion and metastasis remain unclear. Here, we identify a critical role for ODAM in inducing cancer cell adhesion. ODAM induced RhoA activity and the expression of downstream factors, including Rho‐associated kinase (ROCK). ODAM‐mediated RhoA signalling resulted in actin filament rearrangement by activating PTEN and inhibiting the phosphorylation of ...
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Hye‐Kyung Lee, Han‐Wool Choung, Young‐Il Yang, Hye‐Jung Yoon, In Ae Park, Joo‐Cheol Park Tags: Research Article Source Type: research
Activation of Toll like receptor 3 induces spermatogonial stem cell apoptosis
In conclusion, the results of the present study indicate that activation of TLR3 by PolyI:C induces the SSC apoptosis, which implies that viral infection may interfere with the male germ cell development. Copyright © 2015 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Jianxin Hu, Dalong Song, Guangheng Luo, Suxiong Xu, Ying Cao, Zhaolin Sun Tags: Research Article Source Type: research
Changes in glucose transporter expression and nitric oxide production are associated with liver injury in diabetes
In diabetes mellitus (DM), both hyperglycaemia and hyperlipidaemia can initiate accumulation of fat in the liver, which might be further mediated by inducible nitric oxide synthase. We have studied changes in GLUT1, nitric oxide (NO·) concentration and liver damage in two rat DM models. STZ model was induced by strepozotocin 50 mg/kg. HS model was induced by high‐fat diet and 30 mg/kg streptozotocin. GLUT1 expression was studied by means of real‐time RT‐PCR and immunohistochemistry. Production of NO· was monitored by means of erythrocyte sedimentation rate spectroscopy of Fe‐DETC‐NO complex. Liver damage wa...
Source: Cell Biochemistry and Function - July 1, 2015 Category: Biochemistry Authors: Jeļizaveta Sokolovska, Sergejs Isajevs, Evita Rostoka, Tatjana Sjakste, Ilva Trapiņa, Kristīne Ošiņa, Natalia Paramonova, Nikolajs Sjakste Tags: Research Article Source Type: research
Hsa‐miR‐132 inhibits proliferation of hepatic carcinoma cells by targeting YAP
In this study, we showed that miR‐132 could target YAP gene by using dual‐luciferase reporter system. Further quantitative polymerase chain reaction analysis and western blotting showed that miR‐132 could significantly reduce the expression of YAP at mRNA and protein levels. Results of annexin V‐fluorescein isothiocyanate, 5‐ethynyl‐2′‐deoxyuridine staining and transwell assays showed that miR‐132 significantly promoted the cell apoptosis and effectively inhibited the proliferation and invasion of hepatoma cells. These results indicated that miR‐132 could inhibit the growth of hepatoma cells by targetin...
Source: Cell Biochemistry and Function - June 19, 2015 Category: Biochemistry Authors: Chang‐Jiang Lei, Lei Li, Xia Gao, Jun Zhang, Qing‐Yun Pan, Hao‐Cheng Long, Chun‐Zhou Chen, De‐Fa Ren, Gang Zheng Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 18, 2015 Category: Biochemistry Tags: Issue Information Source Type: research
Comparison of the effect of raw and blanched‐frozen broccoli on DNA damage in colonocytes
This study is the first to report that the consumption of raw broccoli can damage DNA in porcine colonocytes. Copyright © 2015 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 9, 2015 Category: Biochemistry Authors: Anthony Lynn, Zoë Fuller, Andrew R. Collins, Brian Ratcliffe Tags: Research Article Source Type: research
An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin‐like growth factor‐1 from a collagen–glycosaminoglycan scaffold
This study describes a simple, non‐compressive method that is applicable to mammalian or human cartilage and provides a reasonable throughput of samples. Rings of full‐depth articular cartilage slices were derived from human donors undergoing knee replacement for osteoarthritis and a 3 mm core of a collagen/glycosaminoglycan biomimetic scaffold (Tigenix, UK) inserted to create the EVM. Adult osteoarthritis chondrocytes were seeded into the scaffold and cultures maintained for up to 30 days. Ex vivo models were stable throughout experiments, and cells remained viable. Chondrocytes seeded into the EVM attached throug...
Source: Cell Biochemistry and Function - June 9, 2015 Category: Biochemistry Authors: J. Wardale, L. Mullen, D. Howard, S. Ghose, N. Rushton Tags: Research Article Source Type: research
Inhibition of bleomycin‐induced pulmonary fibrosis by bone marrow‐derived mesenchymal stem cells might be mediated by decreasing MMP9, TIMP‐1, INF‐γ and TGF‐β
The study was aimed to investigate the mechanism and administration timing of bone marrow‐derived mesenchymal stem cells (BMSCs) in bleomycin (BLM)‐induced pulmonary fibrosis mice. Thirty‐six mice were divided into six groups: control group (saline), model group (intratracheal administration of BLM), day 1, day 3 and day 6 BMSCs treatment groups and hormone group (hydrocortisone after BLM treatment). BMSCs treatment groups received BMSCs at day 1, 3 or 6 following BLM treatment, respectively. Haematoxylin and eosin and Masson staining were conducted to measure lung injury and fibrosis, respectively. Matrix metallopro...
Source: Cell Biochemistry and Function - June 1, 2015 Category: Biochemistry Authors: Shi‐huan Yu, Li‐jie Liu, Bin Lv, Chun‐li Che, Da‐ping Fan, Li‐feng Wang, Yi‐mei Zhang Tags: Research Article Source Type: research
Quercetin modulates keratoconus metabolism in vitro
Corneal scarring is the result of a disease, infection or injury. The resulting scars cause significant loss of vision or even blindness. To‐date, the most successful treatment is corneal transplantation, but it does not come without side effects. One of the corneal dystrophies that are correlated with corneal scarring is keratoconus (KC). The onset of the disease is still unknown; however, altered cellular metabolism has been linked to promoting the fibrotic phenotype and therefore scarring. We have previously shown that human keratoconus cells (HKCs) have altered metabolic activity when compared to normal human corn...
Source: Cell Biochemistry and Function - June 1, 2015 Category: Biochemistry Authors: Tina B McKay, Akhee Sarker‐Nag, Desiree' Lyon, John M Asara, Dimitrios Karamichos Tags: Research Article Source Type: research
Platelet‐derived growth factor (PDGF) signalling in cancer: rapidly emerging signalling landscape
Platelet‐derived growth factor (PDGF)‐mediated signalling has emerged as one of the most extensively and deeply studied biological mechanism reported to be involved in regulation of growth and survival of different cell types. However, overwhelmingly increasing scientific evidence is also emphasizing on dysregulation of spatio‐temporally controlled PDGF‐induced signalling as a basis for cancer development. We partition this multi‐component review into recently developing understanding of dysregulation PDGF signalling in different cancers, how PDGF receptors are quantitatively controlled by microRNAs. Moreover, we...
Source: Cell Biochemistry and Function - June 1, 2015 Category: Biochemistry Authors: Ammad Ahmad Farooqi, Zahid H. Siddik Tags: Review Article Source Type: research
Circulating miR‐145 is associated with plasma high‐sensitivity C‐reactive protein in acute ischemic stroke patients
Stroke is a major cerebrovascular disease threatening human health and life with high morbidity, disability and mortality. We aimed to find effective biomarkers for the early diagnosis on stroke. Nine previously reported stroke‐associated miRNAs (miR‐21, miR‐23a, miR‐29b, miR‐124, miR‐145, miR‐210, miR‐221, miR‐223 and miR‐483‐5p) were measured by quantitative real time‐PCR, and plasma high‐sensitivity C‐reactive protein (hs‐CRP) and serum interleukin 6 (IL‐6), the pro‐inflammation markers in brain injury, were examined by enzyme‐linked immunosorbent assay in 146 acute ischemic stroke pa...
Source: Cell Biochemistry and Function - June 1, 2015 Category: Biochemistry Authors: Lihua Jia, Fang Hao, Weihua Wang, Yang Qu Tags: Research Article Source Type: research
