Follistatin ‐like protein 1 contributes to dendritic cell and T‐lymphocyte activation in nasopharyngeal carcinoma patients by altering nuclear factor κb and Jun N‐terminal kinase expression
In this study, we explored the impact of FSTL1 on DC activity in nasopharyngeal carcinoma (NPC) patients. The surface expression of CD40, CD86, and HLA‐DR on DCs was analyzed and showed significantly elevated expression levels, indicating DC maturity. After FSTL1 was added to DCs collected from NPC patients (n = 50), controls (n = 47), and healthy donors (n = 10), interferon γ secretion and T‐cell receptor expression in cytotoxic T lymphocytes were also investigated. In the experimental groups, the expression of the critical immune protein nuclear factor (NF)‐κb was upregulated, whereas Jun N‐terminal kinas...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: Hong Wang, Senyong Wu, Shiping Huang, Shaolin Yin, Guilong Zou, Kuan'en Huang, Zhe Zhang, Anzhou Tang, Wensheng Wen Tags: RESEARCH ARTICLE Source Type: research
RUNX3 inhibits laryngeal squamous cell carcinoma malignancy under the regulation of miR ‐148a‐3p/DNMT1 axis
In this study, we reported that the RUNX3 was significantly downregulated and highly methylated in LSCC compared with their matched normal. The enforced expression of RUNX3 inhibited LSCC cell migration, invasion, and proliferation, whereas the inhibition of RUNX3 did the opposite. We identified that RUNX3 was regulated by miR‐148a‐3p and found that the expression level of miR‐148‐3p was significantly decreased and positively related with the expression of RUNX3 in LSCC. We also identified that DNA methyltransferase enzyme DNA (cytosine‐5‐)‐methyltransferase 1 (DNMT1) was targeted by miR‐148a‐3p in LSCC. ...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: Su Jili, Lu Eryong, Lu Lijuan, Zhang Chao Tags: RESEARCH ARTICLE Source Type: research
N ‐methyl‐N‐nitrosourea induces retinal degeneration in the rat via the inhibition of NF‐κB activation
This study aimed to investigate the role of nuclear factor κB (NF‐κB) in MNU‐induced photoreceptor degeneration. Adult rats received a single intraperitoneal injection of MNU (60 mg/kg bodyweight). Hematoxylin and eosin staining demonstrated progressive outer nuclear layer (ONL) loss after MNU treatment. Transmission electron microscopy revealed nuclear pyknosis, chromatin margination in the photoreceptors, increased secondary lysosomes, and lobulated retinal‐pigmented epithelial cells in MNU‐treated rats. Numerous photoreceptor cells in the ONL showed positive TUNEL staining and apoptosis rate peaked at 24 hou...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: Yu Xiong, Hong ‐pei Ji, Wei‐tao Song, Ye‐Wei Yin, Chao‐hua Xia, Bei Xu, Yi Xu, Xiao‐bo Xia Tags: RESEARCH ARTICLE Source Type: research
Comparative expression analysis of hypoxia ‐inducible factor‐alpha and its natural occurring antisense in breast cancer tissues and adjacent noncancerous tissues
Hypoxia‐inducible factors (HIFs) have been shown to be upregulated in tumor tissues and linked with tumor progression and metastasis in breast cancer. Among regulatory mechanisms for HIF expression is a natural occurring antisense named aHIF, which has been shown to be overexpressed in breast cancer and influence the level of the HIF‐1α transcript. In the present study, we analyzed the expression of HIF‐1α and aHIF in breast cancer tissues versus adjacent noncancer tissues (ANCTs) in relation with the clinical and biological behavior of the tumors. aHIF has been shown to be expressed in 67.4% of invasive ductal car...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: Behnoosh Tasharrofi, Mohammad Soudyab, Elahe Nikpayam, Mostafa Iranpour, Reza Mirfakhraie, Shaghayegh Sarrafzadeh, Lobat Geranpayeh, Eznollah Azargashb, Arezou Sayad, Soudeh Ghafouri ‐Fard Tags: RESEARCH ARTICLE Source Type: research
From obesity to cancer: a review on proposed mechanisms
Nowadays, obesity is considered as a serious and growing global health problem. It is documented that the overweight and obesity are major risk factors for a series of noncommunicable diseases, and in recent years, the obesity‐cancer link has received much attention. Numerous epidemiological studies have shown that obesity is associated with increased risk of several cancer types, including colon, breast, endometrium, liver, kidney, esophagus, gastric, pancreatic, gallbladder, and leukemia, and can also lead to poorer treatment.
We review here the epidemiological and experimental evidences for the association between obe...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: Zoya Tahergorabi, Majid Khazaei, Mitra Moodi, Elham Chamani Tags: REVIEW ARTICLE Source Type: research
Effect of bafilomycin and NAADP on membrane ‐associated ATPases and respiration of isolated mitochondria of the murine Nemeth‐Kellner lymphoma
The goal of the study was to estimate the effect of a selective V‐type H+‐ATPase inhibitor bafilomycin A1 and nicotinic acid adenine dinucleotide phosphate (NAADP) on energetic processes in NK/Ly cell by directly measuring the respiration of isolated mitochondria and ATPase activities.NAADP (7 μM) increased the activity of Na+/K+‐ATPase in the postmitochondrial fraction of NK/Ly cells, but lower concentration of NAADP decreased it (0.1 and 1 μM). The increase the activity of plasma membrane Ca2+ ATPase (PMCA) under NAADP application (1 and 7 μM) was observed. However, NAADP (1 μM) decreased activities of sarc...
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Authors: V. Hreniukh, S. Bychkova, O. Kulachkovsky, A. Babsky Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - September 30, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Differentiation of human foreskin fibroblast ‐derived induced pluripotent stem cells into hepatocyte‐like cells
The aim of this study was to investigate the differentiation potential of induced pluripotent stem cells (iPSCs) derived from human foreskin fibroblasts (HFFs) into hepatocyte‐like cells (HLCs). The iPSCs were firstly induced by transduction of OCT4, SOX2, KLF4, and c‐MYC into HFFs using retrovirus. Afterwards, expressions of pluripotency factors were identified by semiquantitative reverse transcription–polymerase chain reaction and immunofluorescence staining, and karyotype, embryoid, and teratoma were observed by microscope. Then, iPSCs were gradually differentiated into endoderm cells, hepatic progenitor cells, an...
Source: Cell Biochemistry and Function - August 28, 2016 Category: Biochemistry Authors: Jianjun Wang, Ping Zhao, Zhihong Wan, Xueyuan Jin, Yongqian Cheng, Tao Yan, Song Qing, Ning Ding, Shaojie Xin Tags: RESEARCH ARTICLE Source Type: research
The Ganglioside GM ‐1 Inhibits Bupivacaine‐Induced Neurotoxicity in Mouse Neuroblastoma Neuro2a Cells
ConclusionBupivacaine induced neurotoxicity by activating apoptosis via the mitochondrial pathway, and this was inhibited by GM‐1 pretreatment. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 24, 2016 Category: Biochemistry Authors: Yujie Liang, Jiemei Ji, Yunan Lin, Yajun He, Jingchen Liu Tags: RESEARCH ARTICLE Source Type: research
MicroRNA signatures predict dysregulated vitamin D receptor and calcium pathways status in limb girdle muscle dystrophies (LGMD) 2A/2B
miRNA expression profile and predicted pathways involved in selected limb‐girdle muscular dystrophy (LGMD)2A/2B patients were investigated. A total of 187 miRNAs were dysregulated in all patients, with six miRNAs showing opposite regulation in LGMD2A versus LGMD2B patients. Silico analysis evidence: (1) a cluster of the dysregulated miRNAs resulted primarily involved in inflammation and calcium metabolism, and (2) two genes predicted as controlled by calcium‐assigned miRNAs (Vitamin D Receptor gene and Guanine Nucleotide Binding protein beta polypeptide 1gene) showed an evident upregulation in LGMD2B patients, in accor...
Source: Cell Biochemistry and Function - August 24, 2016 Category: Biochemistry Authors: M. Aguennouz, C. Lo Giudice, N. Licata, C. Rodolico, O. Musumeci, M. Fanin, A. Migliorato, M. Ragusa, V. Macaione, R. M. Di Giorgio, C. Angelini, A. Toscano Tags: Research Article Source Type: research
Study of differentiated human umbilical cord –derived mesenchymal stem cells transplantation on rat model of advanced parkinsonism
The aim of this study was to explore the curative effect of differentiated human umbilical cord–derived mesenchymal stem cells (hUC‐MSCs) transplantation on rat of advanced Parkinson disease (PD) model. Human umbilical cord–derived mesenchymal stem cells were cultured and induced differentiation in vitro. The PD rats were established and allocated randomly into 2 groups: differentiated hUC‐MSCs groups and physiological saline groups (the control group). Rotation test and immunofluorescence double staining were done. The result showed that hUC‐MSCs could differentiate into mature dopamine neurons. Frequency of rot...
Source: Cell Biochemistry and Function - August 24, 2016 Category: Biochemistry Authors: Zhaowei Wang, Aimin Chen, Shengjuan Yan, Chengyan Li Tags: RAPID COMMUNICATION Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 24, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Myosin light chain kinase steady ‐state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates
Myosin light chain kinase (MLCK) phosphorylates S19 of the myosin regulatory light chain (RLC), which is required to activate myosin's ATPase activity and contraction. Smooth muscles are known to display plasticity in response to factors such as inflammation, developmental stage, or stress, which lead to differential expression of nonmuscle and smooth muscle isoforms. Here, we compare steady‐state kinetics parameters for phosphorylation of different MLCK substrates: (1) nonmuscle RLC, (2) smooth muscle RLC, and heavy meromyosin subfragments of (3) nonmuscle myosin IIB, and (4) smooth muscle myosin II. We show that MLCK h...
Source: Cell Biochemistry and Function - August 15, 2016 Category: Biochemistry Authors: Diego B. Alcala, Brian D. Haldeman, Richard K. Brizendine, Agata K. Krenc, Josh E. Baker, Ronald S. Rock, Christine R. Cremo Tags: RESEARCH ARTICLE Source Type: research
SOD1 dimerization monitoring using a novel split NanoLuc, NanoBit
In the present study, we applied a highly sensitive NanoLuc‐based technology to understand the status of superoxide dismutase 1 (SOD1) within mammalian cells. Two fragments of NanoLuc (NanoBit), large N‐terminal and small C‐terminal regions, were fused with wild‐type (wt) and mutant human SOD1 (hSOD1) genes and transfected into cells. Luciferase activity through NanoBit assembly was only detected in NanoBit‐tagged wtSOD1‐expressing cells. Furthermore, the developed NanoLuc system was used to investigate the role of protein‐protein interactions in the pathogenesis of amyotrophic lateral sclerosis (ALS). In add...
Source: Cell Biochemistry and Function - July 31, 2016 Category: Biochemistry Authors: Kentaro Oh ‐hashi, Yoko Hirata, Kazutoshi Kiuchi Tags: RESEARCH ARTICLE Source Type: research
MiR ‐24‐3p enhances cell growth in hepatocellular carcinoma by targeting metallothionein 1M
Dysregulation of microRNAs has been demonstrated to contribute to malignant progression of cancers, including hepatocellular carcinoma (HCC). MiR‐24‐3p was previously reported to be significantly upregulated in HCC. However, the potential role and mechanism of action of miR‐24‐3p in the initiation and progression of HCC remain largely unknown. Quantitative reverse transcription polymerase chain reaction demonstrated that miR‐24‐3p was significantly upregulated in HCC tumor tissues compared with nontumor tissues. The cell viability, colony formation assay, and tumorigenicity assays in nude mice showed that miR...
Source: Cell Biochemistry and Function - July 31, 2016 Category: Biochemistry Authors: Xiaogang Dong, Wei Ding, Jianwei Ye, Dong Yan, Feng Xue, Lin Xu, Jiwei Yin, Wenjia Guo Tags: RESEARCH ARTICLE Source Type: research
