Dietary consumption of monosodium L ‐glutamate induces adaptive response and reduction in the life span of Drosophila melanogaster
In conclusion, consumption of MSG for 5 days by D. melanogaster induced adaptive response, but long‐term exposure reduced life span of flies. This study may therefore have public health significance in humans, and thus, moderate consumption of MSG is advocated by the authors. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - February 28, 2017 Category: Biochemistry Authors: Amos O. Abolaji, Charles O. Olaiya, Oluwagbenga J. Oluwadahunsi, Ebenezer O. Farombi Tags: RESEARCH ARTICLE Source Type: research
Strain uses gap junctions to reverse stimulation of osteoblast proliferation by osteocytes
Identifying mechanisms by which cells of the osteoblastic lineage communicate in vivo is complicated by the mineralised matrix that encases osteocytes, and thus, vital mechanoadaptive processes used to achieve load‐bearing integrity remain unresolved. We have used the coculture of immunomagnetically purified osteocytes and primary osteoblasts from both embryonic chick long bone and calvariae to examine these mechanisms. We exploited the fact that purified osteocytes are postmitotic to examine both their effect on proliferation of primary osteoblasts and the role of gap junctions in such communication. We found that chick...
Source: Cell Biochemistry and Function - January 11, 2017 Category: Biochemistry Authors: Rosemary F.L. Suswillo, Behzad Javaheri, Simon C.F. Rawlinson, Gary P. Dowthwaite, Lance E. Lanyon, Andrew A. Pitsillides Tags: RESEARCH ARTICLE Source Type: research
Stimulation of neurotrophic factors and inhibition of proinflammatory cytokines by exogenous application of triiodothyronine in the rat model of ischemic stroke
There is a positive relation between decreases of triiodothyronine (T3) amounts and severity of stroke. The aim of this study was to evaluate the effect of exogenous T3 application on levels of neurogenesis markers in the subventricular zone. Cerebral ischemia was induced by middle cerebral artery occlusion in male Wistar rats. There were 4 experimental groups: sham, ischemic, vehicle, and treatment. Rats were injected with T3 (25 μg/kg, IV injection) at 24 hours after ischemia. Animals were sacrificed at day 7 after ischemia. There were high levels of brain‐derived neurotrophic factor, nestin, and Sox2 expressions in...
Source: Cell Biochemistry and Function - January 11, 2017 Category: Biochemistry Authors: Fatemeh Sabbaghziarani, Keywan Mortezaee, Mohammad Akbari, Iraj Ragerdi Kashani, Mansooreh Soleimani, Gholamreza Hassanzadeh, Adib Zendedel Tags: RESEARCH ARTICLE Source Type: research
Mechanistic insight into nuclear receptor ‐mediated regulation of bile acid metabolism and lipid homeostasis by grape seed procyanidin extract (GSPE)
Dietary procyanidins have emerged as important bioactive components that regulate various metabolic pathways to maintain homeostasis. Grape seed procyanidin extract (GSPE), in particular, has demonstrated regulatory effects on bile acid and lipid metabolism in vivo. While numerous studies in rodent models have shown the potent hypolipidemic action of grape seed extracts, human studies have shown inconsistent results. This review will focus on the molecular mechanisms underlying the hypolipidemic actions of GSPE identified to date, specifically highlighting the effects exerted via nuclear receptors. Such evidence may provid...
Source: Cell Biochemistry and Function - January 11, 2017 Category: Biochemistry Authors: Laura E. Downing, Daniel Edgar, Patricia A. Ellison, Marie ‐Louise Ricketts Tags: REVIEW ARTICLE Source Type: research
Small animal models to understand pathogenesis of osteoarthritis and use of stem cell in cartilage regeneration
Osteoarthritis (OA) is one of the most common diseases, which affect the correct functionality of synovial joints and is characterized by articular cartilage degradation. Limitation in the treatment of OA is mostly due to the very limited regenerative characteristic of articular cartilage once is damaged. Small animal models are of particular importance for mechanistic analysis to understand the processes that affect cartilage degradation. Combination of joint injury techniques with the use of stem cells has been shown to be an important tool for understanding the processes of cartilage degradation and regeneration. Implem...
Source: Cell Biochemistry and Function - January 11, 2017 Category: Biochemistry Authors: Virginia Piombo Tags: REVIEW ARTICLE Source Type: research
Apoptotic effects of bovine apo ‐lactoferrin on HeLa tumor cells
Lactoferrin (Lf), a cationic iron‐binding glycoprotein of 80 kDa present in body secretions, is known as a compound with marked antimicrobial activity. In the present study, the apoptotic effect of iron‐free bovine lactoferrin (apo‐bLf) on human epithelial cancer (HeLa) cells was examined in association with reactive oxygen species and glutathione (GSH) levels. Apoptotic effect of iron‐free bovine lactoferrin inhibited the growth of HeLa cells after 48 hours of treatment while the diferric‐bLf was ineffective in the concentration range tested (from 1 to 12.5 μM). Western blot analysis showed that key apoptotic...
Source: Cell Biochemistry and Function - January 2, 2017 Category: Biochemistry Authors: Carla Luzi, Fabrizia Brisdelli, Roberto Iorio, Argante Bozzi, Veronica Carnicelli, Antonio Di Giulio, Anna Rita Lizzi Tags: RESEARCH ARTICLE Source Type: research
MiR ‐199a‐5p suppresses tumorigenesis by targeting clathrin heavy chain in hepatocellular carcinoma
In this study, we investigated the expression and possible role of miR‐199a‐5p in HCC. The expression of miR‐199a‐5p was measured by quantitative RT‐PCR in HCC. The effect of miR‐199a‐5p was evaluated by cell viability and colony formation assays in HCC cell lines and tumor cell growth assay in xenograft nude mice. Quantitative real time PCR results showed that miR‐199a‐5p was down‐regulated in 77.9 % (67/86) of HCC tissues compared with adjacent nontumor tissues. MiR‐199a‐5p mimic reduced cell viability and colony formation by induction of cell arrest in HCC cell lines and inhibited tumor cell grow...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Guo ‐hao Huang, Hong Shan, Dan Li, Bin Zhou, Peng‐fei Pang Tags: RESEARCH ARTICLE Source Type: research
RIP3 deficiency exacerbates inflammation in dextran sodium sulfate ‐induced ulcerative colitis mice model
Ulcerative colitis (UC) is a chronic intestinal inflammatory disease. The receptor‐interacting protein kinase 3 (RIP3) was reported to be involved in many inflammatory disease. However, the mechanism of RIP3 in the pathogenesis of UC is still unclear. To investigate the effects and possible mechanism of RIP3 in UC pathogenesis, RIP3‐/‐ mice was used in dextran sulfate sodium (DSS)‐induced colitis model. It was found that by DSS‐induced colitis, RIP3‐/‐ mice showed significantly enhanced colitis symptoms, including increased weight loss, colon shortening, and colonic mucosa damage and severity, but decreased p...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Yu ‐Lin Xu, Hai‐Lin Tang, Shi‐Ying Zhu, Hao‐Ran Peng, Zhong‐Tian Qi, Wen Wang Tags: RESEARCH ARTICLE Source Type: research
Expression, subcellular localisation, and possible roles of dipeptidyl peptidase 9 (DPP9) in murine macrophages
In this study, we compared the messenger RNA expression profile of DPP9 to that of the related DPP8 and DPPIV in murine haematopoietic and lymphatic tissues. A similar order of expression levels was observed for all 3 peptidases: peritoneal macrophages < bone marrow < spleen ≤ lymph nodes. Also, we examined the subcellular localisation of DPP9 and its possible role(s) in J774 cell line of macrophage origin. DPP9 was dominantly expressed intracellularly. DPPIV‐like enzymatic activity was mostly present in cytoplasm, but also in cell membranes and organelles/vesicles. Decreased expression of DPP9 was observed...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Emilija Zapletal, Barbara Cupic, Jelka Gabrilovac Tags: RESEARCH ARTICLE Source Type: research
Paracrine effect of CXCR4 ‐overexpressing mesenchymal stem cells on ischemic heart injury
This study aims to investigate the MSCCX4‐derived paracrine cardio‐protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided into 3 groups: MSC only, MSCCX4, and CXCR4 gene‐specific siRNA‐transduced MSC. Mesenchymal stem cells were exposed to hypoxia, and then MSCs‐conditioned culture medium was incubated with neonatal and adult cardiomyocytes, respectively. Cell proliferation–regulating genes were assessed by real‐time polymerase chain reaction (RT‐PCR). In vitro: The number of cardiomyocytes undergoing DNA synthesis, cytokinesis, and mitosis was increased to...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Shi ‐Zheng Wu, Ying‐Lan Li, Wei Huang, Wen‐Feng Cai, Jialiang Liang, Christian Paul, Lin Jiang, Zhi‐Chao Wu, Meifeng Xu, Ping Zhu, Yigang Wang Tags: RESEARCH ARTICLE Source Type: research
Characterization of E ‐NTPDase (EC 3.6.1.5) activity in hepatic lymphocytes: A different activity profile from peripheral lymphocytes
The activity of ectonucleoside triphosphate diphosphohydrolase (E‐NTPDase; EC 3.6.1.5) was characterized in hepatic lymphocytes (HL) of rats. For this purpose, a specific method for the isolation of lymphocytes from hepatic tissue was developed. Subsequently, E‐NTPDase activity of rat HL was compared with that of rat peripheral lymphocytes. The HL showed high cell count and viability. Also, the characterization test revealed that the optimal E‐NTPDase activities were attained at 37°C and pH 8.0 in the presence of Ca2+. In addition, in the presence of specific E‐NTPDase inhibitors (20mM sodium azide and 0.3mM sura...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Pedro H. Doleski, Stephen A. Adefegha, Fernanda L. Cabral, Daniela B.R. Leal Tags: RESEARCH ARTICLE Source Type: research
Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high ‐glucose condition within the diabetic range
To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia‐induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells—especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high‐glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours. In addition to a reduction in the rate of cell viability ...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Aysa Rezabakhsh, Elahe Nabat, Mina Yousefi, Soheila Montazersaheb, Omid Cheraghi, Amir Mehdizadeh, Farzaneh Fathi, Ali Akbar Movassaghpour, Nasrin Maleki ‐Dizaji, Reza Rahbarghazi, Alireza Garjani Tags: RESEARCH ARTICLE Source Type: research
Determine exogenous human DDAH2 gene function in rabbit bone marrow –derived endothelial progenitor cells in vitro
In this study, we isolated rabbit bone marrow–derived EPCs to further manipulation and overexpression of dimethylarginine dimethylaminohydrolase (DDAH) in EPCs. Isolated EPCs were cultured, expanded in endothelial basal medium. Morphology of EPCs and expression levels of surface markers detected using immunocytochemistry staining and through the use of flow cytometery. Endothelial progenitor cells were transfected with plasmid vectors expressing human DDAH2 (DDAH2‐EPCs). Three days after gene transfer, positive transfected‐EPCs proliferation and DDAH activity were assayed. We observed colonies conformation and endoth...
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Sara Shoeibi, Shabnam Mohammadi, Hamid Reza Sadeghnia, Elahe Mahdipour, Majid Ghayour ‐Mobarhan Tags: RESEARCH ARTICLE Source Type: research
Tumor necrosis factor suppresses interleukin 10 in peripheral B cells via upregulating Bcl2 ‐like protein 12 in patients with inflammatory bowel disease
In conclusion, Bcl2L12 mediates the effects of TNF to suppress the expression of IL‐10 in B cells. The data suggest that Bcl2L12 may be a therapeutic target for the treatment of IBD. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Authors: Xiutian Guo, Mao ‐Gang Li, Shan‐Shan Li, Feng‐Hua Liu, Zhan‐Ju Liu, Ping‐Chang Yang Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 31, 2016 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
