Down‐expression of miR‐154 suppresses tumourigenesis in CD133+ glioblastoma stem cells

Glioblastoma multiforme (GBM) is the most common and aggressive form of brain cancer. Evidences have suggested that CD133 is a marker for a subset of glioblastoma cancer stem cells. However, whether miRNA plays a critical role in CD133+ GBM is poorly understood. Here, we identified that miR‐154 was upregulated in CD133+ GBM cell lines. Knockdown of miR‐154 remarkably suppressed proliferation and migration of CD133+ GBM cells. Further study found that PRPS1 was a direct target of miR‐154 in CD133+ GBM cells. Overexpression of PRPS1 exhibited similar effects as miR‐154 knockdown in CD133+ GBMs. Our study identified miR‐154 as a previously unrecognized positive regulator of proliferation and migration in CD133+ GBM cells and a potentially therapeutic target of GBMs. Copyright © 2016 John Wiley & Sons, Ltd.
Source: Cell Biochemistry and Function - Category: Biochemistry Authors: Tags: Research Article Source Type: research