Analysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosis
In this study, we analyzed gene expression in the endocervical epithelium in women with and without moderate or severe endometriosis. Using RT2 Profiler PCR Arrays, we analyzed gene expression in endocervical epithelial cells from women with deep endometriosis (n = 4) and healthy women (n =6). Nine genes were identified as being upregulated: 5 cell cycle genes (cyclin B1 [CCNB1], cyclin G1 [CCNG1], cullin 1 [CUL1], general transcription factor IIH, polypeptide 1 [GTF2H1], and proliferating cell nuclear antigen [PCNA]), 3 cytokine genes (C3, chemokine (C-C motif) ligand 21 [CCL21], and chemokine (C-X-C motif) ligand 14 [CXC...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Kopelman, A., Girao, M. J. B. C., Bonetti, T. C. S., Carvalho, C. V., da Silva, I. D. C. G., Schor, E. Tags: Original Articles Source Type: research
Correlation Between the Clinical Parameters and Tissue Phenotype in Patients Affected by Deep-Infiltrating Endometriosis
The current study aimed to identify and validate an applicable immunohistochemistry panel including Ki-67, c-MYC, estrogen receptor-α (ER-α), and progesterone receptor isoforms A/B (PR-A/B) in correlation with clinicopathological parameters in patients affected by deep infiltrating endometriosis. Tissue microarrays were prepared from a cohort of 113 patients. Phenotypic profile of the panel molecules was evaluated in glands and stroma in parallel with microvessels and stroma density measurements. Principal component analysis was performed on 8 immunohistochemical variables, 2 histological variables, and 8 subgr...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Vinci, G., Arkwright, S., Audebourg, A., Radenen, B., Chapron, C., Borghese, B., Dousset, B., Mehats, C., Vaiman, D., Vacher-Lavenu, M.-C., Gogusev, J. Tags: Original Articles Source Type: research
Pregestational Obesity-Induced Embryopathy
Conclusion:
The model of obesity induced with MSG reproduces the maternal obesity-induced teratogenesis. The hypertriglyceridemia observed in MSG obese pregnant rats could be related to increased birth defect. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Suarez-Roman, G., Fernandez-Romero, T., Perera-Calderin, A. J., Rodriguez-Sosa, V. M., Arranz, C., Hernandez, S. C. Tags: Original Articles Source Type: research
Effects of Exenatide on Metabolic Changes, Sexual Hormones, Inflammatory Cytokines, Adipokines, and Weight Change in a DHEA-Treated Rat Model
This study was performed to evaluate the effect of exenatide on metabolic changes, sexual hormones, inflammatory cytokines, adipokines, and weight changes in a dehydroepiandrosterone (DHEA)-treated rat model. After the model was produced by daily subcutaneous injections of DHEA, rats were given metformin (265 mg/kg), exenatide (10 μg/kg), and saline (1 mL). One group served as a control group. Blood samples and ovarian tissues were removed and prepared for biochemical and hormonal analyses. Exenatide significantly reduced body weight and insulin, testosterone, interleukin 6 (IL-6), PEDF, and visfatin levels. Exenatide a...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Sun, L., Ji, C., Jin, L., Bi, Y., Feng, W., Li, P., Shen, S., Zhu, D. Tags: Original Articles Source Type: research
Endometrial BCL6 Overexpression in Eutopic Endometrium of Women With Endometriosis
The objective of this study was to examine B-cell CLL/lymphoma 6 (BCL6) expression in human eutopic endometrium across the menstrual cycle in women with and without endometriosis and to establish a cutoff for future studies. This design was a series of case–control studies in tertiary University teaching hospitals. We examined BCL6 expression by messenger RNA and immunohistochemically in prospectively collected samples in both the proliferative (P) and the secretory phases. BCL6 is minimally increased in the mid-secretory phase of the menstrual cycle compared to the P phase in normal patients. BCL6 protein expression...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Evans-Hoeker, E., Lessey, B. A., Jeong, J. W., Savaris, R. F., Palomino, W. A., Yuan, L., Schammel, D. P., Young, S. L. Tags: Original Articles Source Type: research
FMR1 CGG Repeats: Reference Levels and Race-Ethnic Variation in Women With Normal Fertility (Study of Womens Health Across the Nation)
This study indicates the need to control for race–ethnicity in FMR1 ovarian aging research and provides race–ethnic population data for females separated by allele. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Pastore, L. M., Manichaikul, A., Wang, X. Q., Finkelstein, J. S. Tags: Original Articles Source Type: research
Surgical History and the Risk of Endometriosis: A Hospital-Based Case-Control Study
This study was undertaken to test the hypothesis that surgery increases the risk of endometriosis. We recruited 208 patients with ovarian endometrioma and 212 age-matched patients with ovarian teratoma and retrieved information on the history of any surgical procedures after menarche, grouped by laparotomy, laparoscopy, gynecologically related procedures, cesarean section, and surgeries performed on torso and extremities was recorded. We then evaluated the association, if any, between endometriosis and history of surgical procedures. Cases and controls were comparable with respect to age, marital status, education level, a...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Liu, X., Long, Q., Guo, S.-W. Tags: Original Articles Source Type: research
Interceed and Estrogen Reduce Uterine Adhesions and Fibrosis and Improve Endometrial Receptivity in a Rabbit Model of Intrauterine Adhesions
In conclusion, Interceed and estrogen reduce adhesions and tissue fibrosis and improve ER in a rabbit model and may be novel therapeutic approaches for infertility resulting from IUA. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Cai, H., Li, H., He, Y. Tags: Original Articles Source Type: research
Phenotypic and Functional Characterization of Mesenchymal Stem/Multipotent Stromal Cells From Decidua Parietalis of Human Term Placenta
In this study, we characterized MSC isolated from the maternal part of human term placenta. The MSCs were isolated from the decidua parietalis (DPMSCs) of human placenta using a digestion method and characterized by colony-forming unit assay and the expression of MSC markers by flow cytometry technique. In addition, DPMSC differentiation into the 3 mesenchymal lineages was also performed. Moreover, the gene and protein expression profiles of DPMSCs were identified by real-time polymerase chain reaction and flow cytometry techniques, respectively. Furthermore, proteins secreted by DPMSCs were detected by sandwich enzyme-lin...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Abumaree, M. H., Abomaray, F. M., Alshehri, N. A., Almutairi, A., AlAskar, A. S., Kalionis, B., Al Jumah, M. A. Tags: Original Articles Source Type: research
Preeclampsia and Inflammatory Preterm Labor Alter the Human Placental Hematopoietic Niche
Conclusion:
Placental HSPCs likely arise from hematopoietic niches comprised RUNX1+ mesenchyme and vascular endothelium. Pregnancy complications that result in preterm birth differentially affect placental HSPC localization and RUNX1 expression. Our results support previous findings that inflammation positively regulates hematopoiesis. We present new evidence that hemogenic endothelium may be active at later stages of human fetal development in the context of inflammation. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Ponder, K. L., Barcena, A., Bos, F. L., Gormley, M., Zhou, Y., Ona, K., Kapidzic, M., Zovein, A. C., Fisher, S. J. Tags: Original Articles Source Type: research
Progesterone, Inflammatory Cytokine (TNF-{alpha}), and Oxidative Stress (H2O2) Regulate Progesterone Receptor Membrane Component 1 Expression in Fetal Membrane Cells
Progesterone receptor membrane component 1 (PGRMC1) is an important novel mediator of progesterone (P4) function in fetal membrane cells. We demonstrated previously that PGRMC1 is differentially expressed in fetal membranes among pregnancy subjects and diminished in preterm premature rupture of membrane subjects. In the current study, we aim to elucidate whether PGRMC1 expression is regulated by P4, tumor necrosis factor α (TNF-α), and H2O2 in fetal membrane cells. Primary cultured membrane cells were serum starved for 24 hours followed by treatments of P4, 17 hydroxyprogesterone caproate, and medroxyprogestero...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Meng, Y., Murtha, A. P., Feng, L. Tags: Original Articles Source Type: research
Endometriosis Is Associated With a Shift in MU Opioid and NMDA Receptor Expression in the Brain Periaqueductal Gray
Studies have examined how endometriosis interacts with the nervous system, but little attention has been paid to opioidergic systems, which are relevant to pain signaling. We used the autotransplantation rat model of endometriosis and allowed to progress for 60 days. The brain was collected and examined for changes in endogenous opioid peptides, mu opioid receptors (MORs), and the N-methyl-d-aspartate subunit receptor (NR1) in the periaqueductal gray (PAG), since both of these receptors can regulate PAG activity. No changes in endogenous opioid peptides in met- and leu-enkephalin or β-endorphin levels were observed wi...
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Torres-Reveron, A., Palermo, K., Hernandez-Lopez, A., Hernandez, S., Cruz, M. L., Thompson, K. J., Flores, I., Appleyard, C. B. Tags: Original Articles Source Type: research
Stresses on Female Mice Impair Oocyte Developmental Potential: Effects of Stress Severity and Duration on Oocytes at the Growing Follicle Stage
Conclusion:
The results suggest that (1) the degree that a stress damages oocytes is the product of duration x severity of the stress; (2) RRS impaired oocyte developmental potential through cumulative effects on growing follicles; and (3) preantral follicles were not as sensitive to stress as antral follicles were. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Gao, Y., Chen, F., Kong, Q.-Q., Ning, S.-F., Yuan, H.-J., Lian, H.-Y., Luo, M.-J., Tan, J.-H. Tags: Original Articles Source Type: research
Fenretinide: A Potential Treatment for Endometriosis
Conclusions:
Fenretinide increases STRA6 expression thereby potentially reversing the pathological loss of retinoid availability. Treatment with this compound induces apoptosis. In vivo treatments decrease lesion volume. Targeting the RA signaling pathway may be a promising novel treatment for women with endometriosis. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Pavone, M. E., Malpani, S. S., Dyson, M., Kim, J. J., Bulun, S. E. Tags: Original Articles Source Type: research
Effects of Prenatal Environmental Exposures on the Development of Endometriosis in Female Offspring
Conclusion:
Certain prenatal environmental exposures might result in the development of endometriosis in female offspring. In addition to known environmental exposures that predispose the development of endometriosis in adulthood, such as dioxin and radiation exposure (animal models), prenatal exposures are of increasing concern. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 8, 2016 Category: Reproduction Medicine Authors: Wei, M., Chen, X., Zhao, Y., Cao, B., Zhao, W. Tags: Reviews Source Type: research
