The Impact of Genetic Variants for Different Physiological Characterization of Type 2 Diabetes Loci on Gestational Insulin Signaling in Nondiabetic Pregnant Chinese Women
We investigate the impact of genetic variants on transiently upregulated gestational insulin signaling. We recruited 1152 unrelated nondiabetic pregnant Han Chinese women (age 28.5 ± 4.1 years; body mass index [BMI] 21.4 ± 2.6 kg/m2) and gave them oral glucose tolerance tests. Matsuda index of insulin sensitivity, homeostatic model assessment of insulin resistance, indices of insulin disposition, early-phase insulin release, fasting state, and 0 to 120 minute’s proinsulin to insulin conversion were used to dissect insulin physiological characterization. Several variants related to β-cell function w...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Liao, S., Liu, Y., Chen, X., Tan, Y., Mei, J., Song, W., Gan, L., Wang, H., Yin, S., Dong, X., Chi, S., Deng, S. Tags: Original Articles Source Type: research
The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis
We hypothesized that repetitive umbilical cord occlusions (UCOs) leading to severe acidemia will stimulate a placental and thereby fetal inflammatory response which will be exacerbated by chronic hypoxemia and low-grade bacterial infection. Chronically instrumented fetal sheep served as controls or underwent repetitive UCOs for up to 4 hours or until fetal arterial pH was <7.00. Normoxic-UCO and hypoxic-UCO fetuses had arterial O2 saturation pre-UCOs of >55% and <55%, respectively, while lipopolysaccharide (LPS)-UCO fetuses received LPS intra-amniotic (2 mg/h) starting 1 hour pre-UCOs. Fetal plasma and amniotic fl...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Xu, A., Matushewski, B., Cao, M., Hammond, R., Frasch, M. G., Richardson, B. S. Tags: Original Articles Source Type: research
Mouse Endometrium Temporal and Spatial Expression mRNA and MicroRNA Associated With Embryo Implantation
Embryo implantation is a dynamic physiological process involving morphological and molecular changes in the endometrium during the pre-receptivity, receptivity, and implantation phases. A comprehensive analysis of messenger RNA (mRNA) and microRNA (miRNA) profiles during implantation will likely provide new clues to elucidate the underlying mechanisms governing embryo implantation. We characterized the mRNA and miRNA transcriptomes using next generation sequencing (NGS) of the endometrium 1 day postcoitum (dpc) and 4dpc and the implantation site (IMS) and inter-implantation (IIM) site of the endometrium on 5dpc. Real-time ...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Chen, K., Chen, X., He, J., Ding, Y., Geng, Y., Liu, S., Liu, X., Wang, Y. Tags: Original Articles Source Type: research
{alpha}-Amylase in Vaginal Fluid: Association With Conditions Favorable to Dominance of Lactobacillus
Vaginal glycogen is degraded by host α-amylase and then converted to lactic acid by Lactobacilli. This maintains the vaginal pH at ≤4.5 and prevents growth of other bacteria. Therefore, host α-amylase activity may promote dominance of Lactobacilli. We evaluated whether the α-amylase level in vaginal fluid is altered in women with bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) and whether its concentration was associated with levels of lactic acid isomers and host mediators. Vaginal fluid was obtained from 43 women with BV, 50 women with VVC, and 62 women with no vulvovaginal disorders. Vag...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Nasioudis, D., Beghini, J., Bongiovanni, A. M., Giraldo, P. C., Linhares, I. M., Witkin, S. S. Tags: Original Articles Source Type: research
Is There a Link Between Expression Levels of Histone Deacetylase/Acetyltransferase in Mouse Sperm and Subsequent Blastocyst Development?
In conclusion, expression of sperm HDAC1 could be considered as a possible predictor of embryo development in mice with high ovarian response. (Source: Reproductive Sciences)
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Kim, J., Kim, J.-H., Jee, B.-C., Suh, C.-S., Kim, S.-H. Tags: Original Articles Source Type: research
Morphological Changes and Expression of Cytokine After Local Endometrial Injury in a Mouse Model
Conclusions:
Local mechanical injury on mouse uteri enhanced endometrial receptivity and improved embryo implantation, which were correlated with the characteristic changes in endometrial morphology and the upregulation of LIF and OSM gene and protein expression. (Source: Reproductive Sciences)
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Zhang, X.-H., Liu, Z.-Z., Tang, M.-X., Zhang, Y.-H., Hu, L., Liao, A.-H. Tags: Original Articles Source Type: research
Mesenchymal Stem Cells Facilitate In Vitro Development of Human Preantral Follicle
Biological folliculogenesis is a lengthy and complicated process, and follicle growth microenvironment is poorly understood. Mesenchymal stem cells (MSCs) have been shown to establish a supportive microenvironment for wound repair, autoimmune diseases amelioration, and tumor development. Therefore, this study is aimed to investigate whether MSCs could help to reconstruct a microenvironment to facilitate the in vitro follicle development. Here we show human MSCs significantly promote the survival rates, increase the growth velocity, and improve the viability of preantral follicles in a dose-dependent manner. Further analyse...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Xia, X., Wang, T., Yin, T., Yan, L., Yan, J., Lu, C., Zhao, L., Li, M., Zhang, Y., Jin, H., Zhu, X., Liu, P., Li, R., Qiao, J. Tags: Original Articles Source Type: research
STAT5 is Activated by Epidermal Growth Factor and Induces Proliferation and Invasion in Trophoblastic Cells
Epidermal growth factor (EGF) is expressed by decidual and trophoblast cells and influences manifold cellular functions during embryo implantation. Thus far, signaling of EGF via Signal Transducer and Activator of Transcription 5 (STAT5) has been only partially investigated. STAT5 stimulates proliferation and cell cycle progression in several cell types. Its dysregulation is associated with pregnancy. The aim of this study was to investigate STAT5 activation and function mediated by EGF in 2 trophoblastic cell lines, namely, HTR8/SVneo and JAR. Additionally, expression of STAT5B messenger RNA (mRNA) in trophoblast models h...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Ospina-Prieto, S., Chaiwangyen, W., Pastuschek, J., Schleussner, E., Markert, U. R., Morales-Prieto, D. M. Tags: Original Articles Source Type: research
Effects of Patient-Controlled Epidural Analgesia on Uterine Electromyography During Spontaneous Onset of Labor in Term Nulliparous Women
Conclusions:
Patient-controlled epidural analgesia initially suppresses uterine EMG and slows cervical dilation thereby prolonging labor. However, the EMG activity recovers with labor progress with no effects on delivery outcomes. (Source: Reproductive Sciences)
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Ye, Y., Song, X., Liu, L., Shi, S.-Q., Garfield, R. E., Zhang, G., Liu, H. Tags: Original Articles Source Type: research
Resveratrol Reduces Myometrial Infiltration, Uterine Hyperactivity, and Stress Levels and Alleviates Generalized Hyperalgesia in Mice With Induced Adenomyosis
In this study, we sought to determine whether resveratrol (RSV), a nonhormonal compound, would suppress the myometrial infiltration, improve pain behavior, lower stress level, improve the expression of some proteins known to be involved in adenomyosis, and reduce uterine contractility in a mice model of adenomyosis. Adenomyosis was induced in 28 female ICR mice neonatally dosed with tamoxifen, while another 12 (group C) were dosed with solvent only, serving as a blank control. Starting from 4 weeks after birth, hotplate test was administrated to all mice every 4 weeks. At the 16th week, all mice with induced adenomyosis we...
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Zhu, B., Chen, Y., Zhang, H., Liu, X., Guo, S.-W. Tags: Review Source Type: research
Fifty Years After Huxley: The Roadmap of Reproductive Medicine Revisited and Updated: The 2015 SRI-Pardi Distinguished Scientist Plenary Lecture of the Society for Reproductive Investigation
(Source: Reproductive Sciences)
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Adashi, E. Y. Tags: Editorial Source Type: research
In the Spotlight
(Source: Reproductive Sciences)
Source: Reproductive Sciences - October 11, 2015 Category: Reproduction Medicine Authors: Maduro, M. R. Tags: In the Spotlight Source Type: research
GPR30 Mediates the Fast Effect of Estrogen on Mouse Blastocyst and its Role in Implantation
Our previous work demonstrated that estrogen could rapidly increase intracellular Ca2+ in dormant mouse blastocysts. The purpose of the present study is to investigate the physiological relevance of G protein-coupled receptor 30 (GPR30) in the fast effect of estrogen on mouse blastocyst and in embryo implantation. We used reverse transcription-polymerase chain reaction, immunofluorescence, embryo coculture with Ishikawa uterine epithelial cell line, and embryo transfer technology to detect the expression of GPR30 in mouse embryos and the nongenomic effects of estrogen via GPR30 on blastocyst. We found that GPR30 is express...
Source: Reproductive Sciences - September 16, 2015 Category: Reproduction Medicine Authors: Yu, L.-l., Qu, T., Zhang, S.-m., Yuan, D.-z., Xu, Q., Zhang, J.-h., He, Y.-p., Yue, L.-m. Tags: Original Articles Source Type: research
Perinatal Administration of a Selective Serotonin Reuptake Inhibitor Induces Impairments in Reproductive Function and Follicular Dynamics in Female Rat Offspring
Conclusions:
This is the first study to investigate the postnatal effects of maternal perinatal exposure to FLX on adult offspring reproduction. We show that genes that regulate serotonin signaling and action in the ovary are altered in prenatally FLX-exposed offspring, which when coupled with increased expression of components of the core Circadian Locomotor Output Cycles Kaput (CLOCK) gene regulatory loop may suggest an interaction between serotonergic signaling and clock gene signaling pathways leading to the altered reproductive phenotype. (Source: Reproductive Sciences)
Source: Reproductive Sciences - September 16, 2015 Category: Reproduction Medicine Authors: Moore, C. J., DeLong, N. E., Chan, K. A., Holloway, A. C., Petrik, J. J., Sloboda, D. M. Tags: Original Articles Source Type: research
Intrauterine Morcellator Devices: The Icon of Hysteroscopic Future or Merely a Marketing Image? A Systematic Review Regarding Safety, Efficacy, Advantages, and Contraindications
We reported data regarding a total of 1185 patients. Concerning polypectomy and myomectomy procedures, IUM systems demonstrated a better outcome in terms of operative time and fluid deficit compared to standard surgical procedures. Complication rates in the inpatient setting were as follows: 0.02% for IUM using Truclear 8.0 (Smith & Nephew Endoscopy, Andover, Massachusetts) and 0.4% for resectoscopic hysteroscopy. No complications were described using Versapoint devices. Office polipectomy reported a total complication rate of 10.1% using Versapoint device (Ethicon Women’s Health and Urology, Somerville, New Jers...
Source: Reproductive Sciences - September 16, 2015 Category: Reproduction Medicine Authors: Noventa, M., Ancona, E., Quaranta, M., Vitagliano, A., Cosmi, E., D'Antona, D., Gizzo, S. Tags: Original Articles Source Type: research
