The Targeted Delivery of Interleukin 4 Inhibits Development of Endometriotic Lesions in a Mouse Model
The objective of this study is to analyze the effect of a targeted therapy with an antibody-based pharmacodelivery of interleukin 4 (F8-IL4) in a mouse model of experimentally induced endometriosis. Endometriosis-like lesions were induced in Balb/c mice. The animals were treated intravenously with F8-IL4 or with untargeted IL4 (KSF-IL4). Twelve days after disease induction, the lesions were isolated. A significant reduction in the number of total lesions/mouse and in the total volume of lesions/mouse was observed in mice treated with F8-IL4 compared to controls (P = .029 and P = .006, respectively), while no difference was...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Quattrone, F., Sanchez, A. M., Pannese, M., Hemmerle, T., Vigano, P., Candiani, M., Petraglia, F., Neri, D., Panina-Bordignon, P. Tags: Original Articles Source Type: research
H3K27me3 is an Epigenetic Mark of Relevance in Endometriosis
This study provides evidence in support of a role of H3K27me3 in the pathogenesis of endometriosis and for EZH2 as a potential therapeutic target for this disease, but more studies are necessary to understand the molecular mechanisms at play. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Colon-Caraballo, M., Monteiro, J. B., Flores, I. Tags: Original Articles Source Type: research
Anti-Mullerian Hormone in Peritoneal Fluid and Plasma From Women With and Without Endometriosis
Anti-Müllerian hormone (AMH) has potential local effects on ovarian function and endometrial tissue, including endometriosis, but its presence in peritoneal fluid is not fully understood. This is a cross-sectional study evaluating AMH in peritoneal fluid and plasma from women with endometriosis (N = 61) and from control women without endometriosis (N = 36). There was a significant correlation between AMH in plasma and peritoneal fluid from both patients with endometriosis (r 2 = .767 [P < .001]) and control participants (r 2 = .647 [P < .001]) less than 45 years of age. Anti-Müllerian hormone declined wit...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Hipp, H., Loucks, T. L., Nezhat, C., Sidell, N., Session, D. R. Tags: Original Articles Source Type: research
Deep Invasive Endometriosis Lesions of the Rectosigmoid May Be Related to Alterations in Cell Kinetics
Conclusions:
Changes in cell proliferation but not in the AI in rectosigmoid endometriosis are indicative of an imbalance in cell kinetics that may lead to the development of the disease. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Bassi, M. A., Arias, V., Filho, N. D., Gueuvoghlanian-Silva, B. Y., Abrao, M. S., Podgaec, S. Tags: Original Articles Source Type: research
Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis
Conclusions:
We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Fassbender, A., Debiec-Rychter, M., Bree, R. V., Vermeesch, J. R., Meuleman, C., Tomassetti, C., Peeraer, K., D'Hooghe, T., Lebovic, D. I. Tags: Original Articles Source Type: research
Evidence for a Hypercoagulable State in Women With Ovarian Endometriomas
This study was undertaken to test the hypothesis that women with ovarian endometriomas are in a hypercoagulable state as manifested by the altered procoagulant factors and higher percentage of activated platelets in their peripheral blood. Two sets of participants were recruited. The first set consisted of 50 premenopausal women with endometriosis and 50 age-matched healthy women, and the second set consisted of 21 women with endometriosis and 17 age-comparable women without endometriosis. For the first set, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen, and other coagu...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Wu, Q., Ding, D., Liu, X., Guo, S.-W. Tags: Original Articles Source Type: research
Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway
Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), a...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Xu, X., Zheng, Q., Zhang, Z., Zhang, X., Liu, R., Liu, P. Tags: Original Articles Source Type: research
Possible Role of {alpha}1-Antitrypsin in Endometriosis-Like Grafts From a Mouse Model of Endometriosis
Previous study indicated that bleeding into the peritoneum may accelerate inflammatory response in endometriosis-like grafts in mice. To identify changes in protein levels in the grafts from mice that underwent unilateral ovariectomy (uOVX), which causes bleeding from ovarian arteries and vein, the grafts were generated by injecting a suspension of human endometrial cells in BALB/c nude female mice, and protein profile changes were compared with non-uOVX control mice. The level of α1-antitrypsin (α1-AT) decreased in grafts from nude mice that underwent uOVX. The levels of phosphorylated Akt, mammalian target of...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Tamura, K., Takashima, H., Fumoto, K., Kajihara, T., Uchino, S., Ishihara, O., Yoshie, M., Kusama, K., Tachikawa, E. Tags: Original Articles Source Type: research
Serum Polyunsaturated Fatty Acids and Endometriosis
Polyunsaturated fatty acids (PUFAs) are fatty acids containing 2 or more double bonds, and they are classified by the location of the last double bond. Omega 3 (n-3) and omega 6 (n-6) PUFAs are obtained through food sources including fatty fish and seed/vegetable oils, respectively, and they are important to a number of physiologic processes including inflammation. Previous work demonstrates suppressive effects of n-3 PUFAs on endometriotic lesions in animal models and decreased risk of endometriosis among women with high n-3 PUFA intake. Thus, we sought to determine the relationship between circulating levels of PUFAs and...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Hopeman, M. M., Riley, J. K., Frolova, A. I., Jiang, H., Jungheim, E. S. Tags: Original Articles Source Type: research
Human Adenomyosis Endometrium Stromal Cells Secreting More Nerve Growth Factor: Impact and Effect
In conclusion, locally increased estrogen levels and inflammation may cause increased NGF production in the uterus of patients with AM. Nerve growth factor stimulated the proliferation and increased aromatase expression of ESCs from AM foci, suggesting NGF might contribute to the pathology and etiology of AM. (Source: Reproductive Sciences)
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Li, Y., Zou, S., Xia, X., Zhang, S. Tags: Original Articles Source Type: research
Integrative Analysis Reveals Regulatory Programs in Endometriosis
In this study, we propose a method to integrate gene expression with TF binding data and protein–protein interactions to construct an integrated regulatory network (IRN) for endometriosis. The IRN has shown that the most regulated gene in endometriosis is RUNX1, which is targeted by 14 of 26 TFs also involved in endometriosis. Using 2 published cohorts, GSE7305 (Hover, n = 20) and GSE7307 (Roth, n = 36) from the Gene Expression Omnibus database, we identified a network of TFs, which bind to target genes that are differentially expressed in endometriosis. Enrichment analysis based on the hypergeometric distribution al...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Yang, H., Kang, K., Cheng, C., Mamillapalli, R., Taylor, H. S. Tags: Original Articles Source Type: research
Pathogenetic Mechanisms of Deep Infiltrating Endometriosis
Endometriosis is a benign gynecologic disease, affecting women of reproductive age associated with chronic pelvic pain, dysmenorrhea, dyspareunia and infertility. Ovarian endometrioma (OMA), superficial peritoneal endometriosis (SPE), and deep infiltrating endometriosis (DIE) are, till now, recognized as major phenotypes. The discussion is to know whether they share the same pathogenetic mechanisms. Till today, DIE is recognized as the most severe clinical form of endometriosis and has a complex clinical management. The DIE lesions have been considered in the present article, without distinguishing between the anterior (bl...
Source: Reproductive Sciences - August 10, 2015 Category: Reproduction Medicine Authors: Tosti, C., Pinzauti, S., Santulli, P., Chapron, C., Petraglia, F. Tags: Review Source Type: research
Activation of Protein C in Human Trophoblasts in Culture and Downregulation of Trophoblast Endothelial Protein C Receptor by TNF-{alpha}
In conclusion, there is a functional protein C pathway on human first-trimester trophoblasts which can be modulated by inflammation. This finding has implications for the pathogenesis and prevention of placenta-mediated obstetric complications. (Source: Reproductive Sciences)
Source: Reproductive Sciences - July 16, 2015 Category: Reproduction Medicine Authors: Faioni, E. M., Fontana, G., Razzari, C., Avagliano, L., Bulfamante, G., Calvi, E., Doi, P., Marconi, A. M. Tags: Original Articles Source Type: research
Plasma miR-127 and miR-218 Might Serve as Potential Biomarkers for Cervical Cancer
In conclusion, we identified the predictive power of 3 plasma miRNAs for cervical cancer, and consequence can be concluded that plasma miR-127 and miR-205 are promising tumor markers for cervical cancer. (Source: Reproductive Sciences)
Source: Reproductive Sciences - July 16, 2015 Category: Reproduction Medicine Authors: You, W., Wang, Y., Zheng, J. Tags: Original Articles Source Type: research
Visfatin/Nampt and SIRT1: Roles in Postterm Delivery in Pregnancies Associated With Obesity
In this study, we sought a role for these proteins in obese pregnant women, who experience more postterm deliveries. Thus, 78 women (26 lean, 24 overweight, and 28 obese) were recruited and maternal blood and placental tissue collected prior to term labor. Plasma levels were measured by enzyme-linked immunosorbent assay and quantitative immunohistochemistry used for placenta. We confirmed maternal plasma interleukin 6 increased according to prepregnancy body mass index (BMI; P < .0001) and showed a linear relationship between BMI and syncytiotrophoblast visfatin/Nampt (P = .021) but not with its levels in maternal plasm...
Source: Reproductive Sciences - July 16, 2015 Category: Reproduction Medicine Authors: Tsai, P.-J. S., Davis, J., Thompson, K., Bryant-Greenwood, G. Tags: Original Articles Source Type: research
