International Journal of Experimental Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

miR‐1, regulated by LMP1, suppresses tumour growth and metastasis by targeting K‐ras in nasopharyngeal carcinoma
In this study we found that restoration of miR‐1 dramatically inhibited cell invasion in vitro, together with tumour growth and metastasis in vivo. Importantly, we found that LMP1, an Epstein–Barr virus (EBV)‐associated protein, suppressed miR‐1 expression. Furthermore, we identified K‐ras as a novel direct target of miR‐1. Our results demonstrated for the first time that miR‐1 was suppressed by LMP1 and its tumour‐suppressive effects were mediated chiefly by repressing K‐ras expression. We propose that miR‐1 could serve as an independent biomarker to identify patients with different clinical characte...
Source: International Journal of Experimental Pathology - January 19, 2016 Category: Pathology Authors: Xi Chen, Jingxuan Shi, Jianwen Zhong, Zhenyun Huang, Xi Luo, Yaping Huang, Shuang Feng, Jianbo Shao, Dabo Liu Tags: Original Article Source Type: research

Staphylococcus epidermidis and biofilm‐associated neutrophils in chronic rhinosinusitis. A pilot study
In this study, biofilms were found in three of 10 patients (mean age = 46 ± 14) with CRS undergoing endoscopic sinus surgery by means of scanning electron microscopy. Unexpectedly, S. epidermidis was the primary isolated bacteria and was also found to be present in all biofilm‐positive mucosa specimens, indicating its pivotal role in the pathogenesis of severe chronic infections associated with biofilm formation. We have also measured the activity of myeloperoxidase (MPO), the most abundant neutrophil enzyme, to demonstrate the presence of neutrophils in the samples tested. Our present results show that the level o...
Source: International Journal of Experimental Pathology - January 14, 2016 Category: Pathology Authors: Janusz Marcinkiewicz, Paweł Stręk, Magdalena Strus, Roman Głowacki, Marta Ciszek‐Lenda, Katarzyna Zagórska‐Świeży, Anna Gawda, Anna Tomusiak Tags: Original Article Source Type: research

Amomum tsao‐ko fruit extract suppresses lipopolysaccharide‐induced inducible nitric oxide synthase by inducing heme oxygenase‐1 in macrophages and in septic mice
This study was designed to assess the anti‐inflammatory effects and the molecular mechanisms of the methanol extract of A. tsao‐ko (AOM) in lipopolysaccharide (LPS)‐induced RAW 264.7 macrophages and in a murine model of sepsis. In LPS‐induced RAW 264.7 macrophages, AOM reduced the production of nitric oxide (NO) by inhibiting inducible nitric oxide synthase (iNOS) expression, and increased heme oxygenase‐1 (HO‐1) expression at the protein and mRNA levels. Pretreatment with SnPP (a selective inhibitor of HO‐1) and silencing HO‐1 using siRNA prevented the AOM‐mediated inhibition of NO production and iNOS e...
Source: International Journal of Experimental Pathology - January 14, 2016 Category: Pathology Authors: Ji‐Sun Shin, Suran Ryu, Dae Sik Jang, Young‐Wuk Cho, Eun Kyung Chung, Kyung‐Tae Lee Tags: Original Article Source Type: research

Remote vs. local ischaemic preconditioning in the rat heart: infarct limitation, suppression of ischaemic arrhythmia and the role of reactive oxygen species
In this study the protective effect of different local ischaemic preconditioning (LIPC) and remote ischaemic preconditioning (RIPC) protocols was compared in the rat model of myocardial ischaemia–reperfusion, using infarct size and ischaemic tachyarrhythmias as end‐points. In addition, the hypothesis that there is involvement of reactive oxygen species (ROS) in the protective signalling by RIPC was tested, again in comparison with LIPC. The animals were subjected to 30‐min coronary occlusion and 90‐min reperfusion. RIPC protocol included either transient infrarenal aortic occlusion (for 5, 15 and 30 min followed b...
Source: International Journal of Experimental Pathology - December 1, 2015 Category: Pathology Authors: Michael M. Galagudza, Dmitry L. Sonin, Timur D. Vlasov, Dmitry I. Kurapeev, Eugene V. Shlyakhto Tags: Original Article Source Type: research

Effect of bisphenol A on morphology, apoptosis and proliferation in the resting mammary gland of the adult albino rat
This study aimed to investigate the influence of BPA on the histological structure of the mammary gland of the adult female albino rat and its effect on epithelial cell proliferation and apoptosis status, in addition to its possible modulating effect on estrogen receptor expression. Thirty female adult albino rats were divided into control and experimental groups. The rats in the experimental group were gavaged with 5 mg/kg BPA daily for 8 weeks. The mammary glands were dissected and processed for histological and immunohistochemical stains for Ki‐67, activated caspase‐3 and estrogen receptor alpha (ER‐α). BPA ind...
Source: International Journal of Experimental Pathology - December 1, 2015 Category: Pathology Authors: Marwa A. A. Ibrahim, Reda H. Elbakry, Naglaa A. Bayomy Tags: Original Article Source Type: research

Efficacy of naringenin against permethrin‐induced testicular toxicity in rats
In conclusion, PM induced testicular toxicity, which was ameliorated by NG co‐administration. However, stoppage of PM exposure was associated with partial recovery. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - December 1, 2015 Category: Pathology Authors: Heba El‐Sayed Mostafa, Samia A. Abd El‐Baset, Asmaa A. A. Kattaia, Rania A. Zidan, Mona M. A. Al Sadek Tags: Original Article Source Type: research

Increased expression of phosphorylated forms of heat‐shock protein‐27 and p38MAPK in macrophage‐rich regions of fibro‐fatty atherosclerotic lesions in the rabbit
Summary We aimed to assess the expression and distribution of Hsp27, pHsp27 (Ser82), p38MAPK and p‐p38MAPK in fibro‐fatty atherosclerotic lesions and the myocardium of hypercholesterolaemic rabbits. Male New Zealand white rabbits were fed a high‐cholesterol diet for 18 weeks, maintaining serum cholesterol at approximately 20 mmol/l over this period. Aortic arch and myocardial tissues were analysed by Western blot, immunohistochemistry and double immunofluorescence. Plasma Hsp27 levels were measured by ELISA. There was a significant increase in the expression of monomeric and dimeric forms of Hsp27, together with pH...
Source: International Journal of Experimental Pathology - December 1, 2015 Category: Pathology Authors: Shahida Shafi, Rosalind Codrington, Lewis Michael Gidden, Gordon Ashley Anthony Ferns Tags: Original Article Source Type: research

Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non‐alcoholic steatohepatitis model, induced by high‐fat and high‐cholesterol diet
SummarySHRSP5/Dmcr is a newly established substrain of stroke‐prone spontaneously hypertensive rat (SHRSP). Recently, high‐fat and high‐cholesterol (HFC) diet‐fed SHRSP5/Dmcr has been reported as a novel rat model of developing hepatic lesions similar to human non‐alcoholic steatohepatitis (NASH). The aim of this study was to investigate the detailed pathological conditions induced by HFC diet in SHRSP5/Dmcr rats using molecular biological methods and morphometric analysis. SHRSP5/Dmcr rats at 6 weeks of age were fed on either HFC diet or stroke‐prone (SP) diet for 2, 4, 6, 8 and 16 weeks and histopathologica...
Source: International Journal of Experimental Pathology - November 30, 2015 Category: Pathology Authors: Yasushi Horai, Hiroyuki Utsumi, Yuko Ono, Toshimitsu Kishimoto, Yuuichi Ono, Atsushi Fukunari Tags: Original Article Source Type: research

High‐level expression of periostin is significantly correlated with tumour angiogenesis and poor prognosis in osteosarcoma
In conclusion, our findings suggest that PN may have an important role in tumour progression and may be used as a prognostic biomarker for patients with osteosarcoma. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - November 30, 2015 Category: Pathology Authors: Fei Hu, Xi‐Fu Shang, Wei Wang, Wei Jiang, Ce Fang, Dong Tan, Hang‐Cheng Zhou Tags: Original Article Source Type: research

Issue Information
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - November 27, 2015 Category: Pathology Tags: Issue Information Source Type: research

Altered expression of CKs 14/20 is an early event in a rat model of multistep bladder carcinogenesis
Summary Cytokeratins (CKs) 14 and 20 are promising markers for diagnosing urothelial lesions and for studying their prognosis and histogenesis. This work aimed to study the immunohistochemical staining patterns of CK14/20 during multistep carcinogenesis leading to papillary bladder cancer in a rat model. Thirty female Fischer 344 rats were divided into three groups: group 1 (control); group 2, which received N‐butyl‐N‐(4‐hydroxybutyl)nitrosamine (BBN) for 20 weeks plus 1 week without treatment; and group 3, which received BBN for 20 weeks plus 8 weeks without treatment. Bladder lesions were classified histologi...
Source: International Journal of Experimental Pathology - October 30, 2015 Category: Pathology Authors: Rui M. Gil da Costa, Paula A. Oliveira, Carmen Vasconcelos‐Nóbrega, Regina Arantes‐Rodrigues, Rosário Pinto‐Leite, Aura A. Colaço, Luis F. Cruz, Carlos Lopes Tags: Original Article Source Type: research

Immunohistochemical expression of matrix metalloproteinase‐1, matrix metalloproteinase‐2 and matrix metalloproteinase‐9, myofibroblasts and Ki‐67 in actinic cheilitis and lip squamous cell carcinoma
In this study immunoexpression of MMP‐1, MMP‐2 and MMP‐9, presence of MFs and the epithelial proliferation index were investigated in actinic cheilitis (AC), lip squamous cell carcinoma (LSCC) and mucocele (MUC). Thirty cases of AC, thirty cases of LSCC and twenty cases of MUC were selected for immunohistochemical investigation of the proteins MMP‐1, MMP‐2, MMP‐9, α‐smooth muscle actin (α‐SMA) and Ki‐67. The MMP‐1 expression in the epithelial component was higher in the AC than the MUC and LSCC. In the connective tissue, the expression was higher in the LSCC. MMP‐2 showed lower epithelial and stroma...
Source: International Journal of Experimental Pathology - October 30, 2015 Category: Pathology Authors: Bianca C. Bianco, Fernanda M. Scotti, Daniella S. C. Vieira, Michelle T. Biz, Renata G. Castro, Filipe Modolo Tags: Original Article Source Type: research

Grape juice concentrate (G8000®) intake mitigates testicular morphological and ultrastructural damage following cadmium intoxication
In conclusion, our results demonstrate that GJC consumption prevented the spermatogenic disruption promoted by cadmium, and thus could be a promising form of therapy against male infertility. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - October 30, 2015 Category: Pathology Authors: Celina A. Lamas, Andrea P.B. Gollücke, Heidi Dolder Tags: Original Article Source Type: research

Effects of the association of diabetes and pulmonary emphysema on cardiac structure and function in rats
In conclusion, alloxan diabetes did not affect RV hypertrophy secondary to chronic emphysema, even in the presence of insulin. Diabetes per se induced left ventricular dysfunction, which was less evident in the presence of RV hypertrophy. Survival rate was substantially reduced as a consequence, at least in part, of the coexistence of RV hypertrophy and diabetic cardiomyopathy. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - October 29, 2015 Category: Pathology Authors: Antonio Di Petta, Rafael Simas, Clebson L. Ferreira, Vera L. Capelozzi, Vera M. C. Salemi, Luiz F. P. Moreira, Paulina Sannomiya Tags: Original Article Source Type: research

Vitamin A dietary supplementation reduces the mortality of velogenic Newcastle disease significantly in cockerels
Summary This project was undertaken to find ways of reducing mortalities and economic losses due to velogenic Newcastle disease (VND) in areas where the disease is enzootic. Four groups of cockerels of 44 birds each were used for this experiment. The birds in groups 1 and 2 received no dietary vitamin A supplementation, whereas groups 3 and 4 received 300 iu and 600 iu of vitamin A per kilogram of commercial feed, respectively, from 1 week of age till the end of the experiment. At 6 weeks of age, the birds in groups 2, 3 and 4 were inoculated intraocularly with a VND virus (duck/Nigeria/Plateau/Kuru/113/1991). The bird...
Source: International Journal of Experimental Pathology - October 28, 2015 Category: Pathology Authors: Godwin Chinedu Okpe, Wilfred Sunday Ezema, Shodeinde Vincent Olumuyiwa Shoyinka, John Osita Afor Okoye Tags: Original Article Source Type: research