International Journal of Experimental Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Coeliac disease: immunogenicity studies of barley hordein and rye secalin ‐derived peptides
Summary Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA‐DQ2 or DQ8 molecules to gluten‐sensitive T cells. A ω‐gliadin/C‐hordein peptide (QPFPQPEQPFPW) and a rye‐derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T‐cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten‐sensitive T‐cell lines obtained from CD small intestinal biopsies....
Source: International Journal of Experimental Pathology - May 31, 2016 Category: Pathology Authors: Widya A. Wahab, Tanja Šuligoj, Julia Ellis, Beatriz Côrtez‐Real, Paul J. Ciclitira Tags: Original Article Source Type: research

Label ‐free Raman spectroscopic imaging to extract morphological and chemical information from a formalin‐fixed, paraffin‐embedded rat colon tissue section
In this study, we provide an extensive characterization of the biochemical signals remaining in processed samples (formalin fixation and paraffin embedding, FFPE) and especially those originating from the anatomical layers of a healthy rat colon. The application of chemometric analytical methods (unsupervised and supervised) was shown to eliminate the need for tissue staining and easily revealed microscopic features consistent with goblet cells and the dense populations of cells within the mucosa, principally via strong nucleic acid signals. We were also able to identify the collagenous submucosa‐ and serosa‐ as well a...
Source: International Journal of Experimental Pathology - May 31, 2016 Category: Pathology Authors: Riana Gaifulina, Andrew Thomas Maher, Catherine Kendall, James Nelson, Manuel Rodriguez ‐Justo, Katherine Lau, Geraint Mark Thomas Tags: Original Article Source Type: research

Seminiferous epithelium damage after short period of busulphan treatment in adult rats and vitamin B12 efficacy in the recovery of spermatogonial germ cells
In this study, we have evaluated the impact of short period of exposure to busulphan on the haemogram and seminiferous epithelium of adult rats, focusing on spermatogonial depletion and Sertoli cell (SC) integrity. We then examined whether vitamin B12 supplementation improves the haematological parameters and spermatogonia number. The animals received 10 mg/kg of busulphan (BuG) or busulfan+vitamin B12 (Bu/B12G) on the first and fourth days of treatment. In H.E.‐stained testicular sections, the areas of the seminiferous tubule (ST) and seminiferous epithelium were measured. The number of spermatogonia in H.E‐stained a...
Source: International Journal of Experimental Pathology - May 31, 2016 Category: Pathology Authors: Sandi Regina Vasiliausha, Fl ávia Luciana Beltrame, Fabiane Santi, Paulo Sérgio Cerri, Breno Henrique Caneguim, Estela Sasso‐Cerri Tags: Original Article Source Type: research

Long ‐term effects of perinatal exposure to low doses of cadmium on the prostate of adult male rats
In conclusion, VP was permanently affected by perinatal Cd exposition, with increased incidence of inflammatory disorders with ageing. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - May 31, 2016 Category: Pathology Authors: Viviane P. Santana, Évila S. Salles, Deborah E. Correa, Bianca F. Gonçalves, Silvana G. Campos, Luiz A. Justulin, Antonio F. Godinho, Wellerson R. Scarano Tags: Original Article Source Type: research

Novel murine tumour models depend on strain and route of inoculation
This study describes variations in tumour growth patterns which occur when changes in the routes of inoculation and mouse strain are used to introduce tumours into established murine model systems that are known to vary in location and aggression. Intraperitoneal, subcutaneous, intravenous and hydrodynamic inoculations of B16F10 cells were compared among CD‐1, C57BL/6 and Balb/c mice. Most surprisingly, allogeneic tumour growth in Balb/c mice after intravenous and hydrodynamic inoculation of B16F10 cells was faster than tumour growth in the syngeneic C57BL/6 mice. These and other variations in the tumour growth patterns ...
Source: International Journal of Experimental Pathology - May 31, 2016 Category: Pathology Authors: Qiang Fu, Andrew Satterlee, Yongjun Wang, Yuhua Wang, Dun Wang, Jingling Tang, Zhonggui He, Feng Liu Tags: Original Article Source Type: research

A 12 ‐month follow‐up study on the preventive effect of oral lansoprazole on acute exacerbation of chronic obstructive pulmonary disease
The objective of this study was to evaluate the preventive effects of oral administration of lansoprazole on acute exacerbation of chronic obstructive pulmonary disease (COPD). Patients with COPD in groups C and D in the stable phase were stratified into a group with neither gastroesophageal reflux nor lansoprazole therapy (group A) and a group subjected to oral lansoprazole therapy (group B1) and a group not subjected to oral lansoprazole therapy (group B2). The frequency scale for the symptoms of gastroesophageal reflux disease (FSSG) questionnaire, COPD assessment test (CAT) questionnaire, pulmonary function test and th...
Source: International Journal of Experimental Pathology - May 1, 2016 Category: Pathology Authors: Wei Xiong, Quan ‐san Zhang, Wei Zhao, Wei Ding, Jin‐ming Liu, Yun‐feng Zhao Tags: Original Article Source Type: research

Different effects of arginine vasopressin on high ‐mobility group box 1 expression in astrocytes isolated from stroke‐prone spontaneously hypertensive rats and congenic SHRpch1_18 rats
Summary Stroke‐prone spontaneously hypertensive rats (SHRSP/Izm) develop severe hypertension and astrocytic oedema following ischaemic stimulation. During ischaemic stress high‐mobility group box 1 (Hmgb1) expression in astrocytes is induced, and subsequently potentiates deterioration of the brain due to ischaemic injury, which manifests as both cerebral inflammation and astrocytic oedema. Arginine vasopressin (AVP) induces brain injury and increases astrocytic swelling. After stroke, Hmgb1 and peroxiredoxin (Prx) are released at different times and activate macrophages in the brain via Toll‐like receptors (Tlr2s). T...
Source: International Journal of Experimental Pathology - April 28, 2016 Category: Pathology Authors: Kazuo Yamagata, Natumi Sone, Sari Suguyama, Toru Nabika Tags: Original Article Source Type: research

Different effects of arginine vasopressin on high‐mobility group box 1 expression in astrocytes isolated from stroke‐prone spontaneously hypertensive rats and congenic SHRpch1_18 rats
Summary Stroke‐prone spontaneously hypertensive rats (SHRSP/Izm) develop severe hypertension and astrocytic oedema following ischaemic stimulation. During ischaemic stress high‐mobility group box 1 (Hmgb1) expression in astrocytes is induced, and subsequently potentiates deterioration of the brain due to ischaemic injury, which manifests as both cerebral inflammation and astrocytic oedema. Arginine vasopressin (AVP) induces brain injury and increases astrocytic swelling. After stroke, Hmgb1 and peroxiredoxin (Prx) are released at different times and activate macrophages in the brain via Toll‐like receptors (Tlr2s). T...
Source: International Journal of Experimental Pathology - April 28, 2016 Category: Pathology Authors: Kazuo Yamagata, Natumi Sone, Sari Suguyama, Toru Nabika Tags: Original Article Source Type: research

Issue Information
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - April 21, 2016 Category: Pathology Tags: Issue Information Source Type: research

Adjunctive role of MMP ‐9 inhibition along with conventional anti‐tubercular drugs against experimental tuberculous meningitis
This study was designed to evaluate whether specific inhibition of MMP‐9 can be beneficial in management of the disease. MMP‐9 levels were inhibited using SB‐3CT or dexamethasone along with conventional drugs for treatment of tuberculous meningitis. Both SB‐3CT and dexamethasone decreased the elevated levels of MMP‐9 in sera and tissues of the infected mice. However, dexamethasone administration had an inhibitory effect on bacillary clearance, while SB‐3CT potentiated the bacillary clearance, suggesting that MMP‐9, if specifically inhibited, can be beneficial in the management of TBM. (Source: International J...
Source: International Journal of Experimental Pathology - March 31, 2016 Category: Pathology Authors: Shahnawaz Majeed, Bishan D. Radotra, Sadhna Sharma Tags: Original Article Source Type: research

Adjunctive role of MMP‐9 inhibition along with conventional anti‐tubercular drugs against experimental tuberculous meningitis
This study was designed to evaluate whether specific inhibition of MMP‐9 can be beneficial in management of the disease. MMP‐9 levels were inhibited using SB‐3CT or dexamethasone along with conventional drugs for treatment of tuberculous meningitis. Both SB‐3CT and dexamethasone decreased the elevated levels of MMP‐9 in sera and tissues of the infected mice. However, dexamethasone administration had an inhibitory effect on bacillary clearance, while SB‐3CT potentiated the bacillary clearance, suggesting that MMP‐9, if specifically inhibited, can be beneficial in the management of TBM. (Source: International J...
Source: International Journal of Experimental Pathology - March 31, 2016 Category: Pathology Authors: Shahnawaz Majeed, Bishan D. Radotra, Sadhna Sharma Tags: Original Article Source Type: research

Phenylbutyrate exerts adverse effects on liver regeneration and amino acid concentrations in partially hepatectomized rats
In conclusion, the delayed onset of liver regeneration and the decrease in BCAA/AAA ratio in blood suggest that phenylbutyrate administration may be disastrous in subjects with acute hepatic injury and BCAA supplementation is needed when phenylbutyrate is used therapeutically. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - March 31, 2016 Category: Pathology Authors: Milan Holecek, Melita Vodenicarovova Tags: Original Article Source Type: research

New approaches in tail‐bleeding assay in mice: improving an important method for designing new anti‐thrombotic agents
This report describes a modified, simple, low‐cost and more sensitive method to determine bleeding patterns and haemoglobin concentration in a tail‐bleeding assay using BALB/c mice and tail tip amputation. The cut tail was immersed in Drabkin's reagent to promote erythrocyte lysis and haemoglobin release, which was monitored over 30 min. The operator was blinded to individual conditions of the mice, which were treated with either saline (NaCl 0.15m), DMSO (0.5%) or clinical anti‐thrombotic drugs. Our experimental protocols showed good reproducibility and repeatability of results when using Drabkin's reagent than wat...
Source: International Journal of Experimental Pathology - March 31, 2016 Category: Pathology Authors: Max Seidy Saito, André Luiz Lourenço, Hye Chung Kang, Carlos Rangel Rodrigues, Lucio Mendes Cabral, Helena Carla Castro, Plínio Cunha Satlher Tags: Original Article Source Type: research

Preventive aerobic training exerts a cardioprotective effect on rats treated with monocrotaline
This study aimed to evaluate the influence of preventive training on hypertrophy, heart function and gene expression of calcium transport proteins in rats with monocrotaline‐induced PAH. Thirty‐two male Wistar rats were randomly divided into four groups: S, sedentary control; T, trained control; SM, sedentary monocrotaline; and TM, trained monocrotaline. The preventive training protocol was performed on a treadmill for 13 weeks, five times/week. The first two weeks were adopted for adaptation to training with gradual increases in speed/time. The speed of the physical training from the third to tenth weeks was graduall...
Source: International Journal of Experimental Pathology - March 31, 2016 Category: Pathology Authors: Francis Lopes Pacagnelli, Ana Karênina Dias Almeida Sabela, Katashi Okoshi, Thaoan Bruno Mariano, Dijon Henrique Salomé Campos, Robson Francisco Carvalho, Antônio Carlos Cicogna, Luiz Carlo Marques Vanderlei Tags: Original Article Source Type: research

Prenatal exposure to ethinylestradiol alters the morphologic patterns and increases the predisposition for prostatic lesions in male and female gerbils during ageing
Summary Ethinylestradiol (EE) is an endocrine disruptor (ED) which acts as an oestrogen agonist; this compound is known as an oral contraceptive. Male and female rodents exposed to EE during critical time points of development, such as in the prenatal period, show alterations in their reproductive tract during adulthood. Few studies have placed an emphasis on the effects of EE during ageing. Thus, this study had as it's objective the analysis of the morphological and immunohistochemical effects of exposure to EE in the prenatal period on ventral male prostate and female prostate of gerbils (Meriones unguiculatus) during ag...
Source: International Journal of Experimental Pathology - February 8, 2016 Category: Pathology Authors: Ana P. S. Perez, Manoel F. Biancardi, Cássia. R. S. Caires, Luiz R. Falleiros‐Junior, Rejane M. Góes, Patricia S. L. Vilamaior, Fernanda C. A. Santos, Sebastião R. Taboga Tags: Original Article Source Type: research