International Journal of Experimental Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Bone marrow cells migrate to the heart and skeletal muscle and participate in tissue repair after Trypanosoma cruzi infection in mice
In conclusion, BMC migrate and contribute to the formation of new resident cells in the heart and skeletal muscle, which can be detected both during the acute and the chronic phase of infection. These findings reinforce the role of BMC in tissue regeneration. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - June 1, 2014 Category: Pathology Authors: Bruno S.d.F. Souza, Carine M. Azevedo, Ricardo S.d. Lima, Carla M. Kaneto, Juliana F. Vasconcelos, Elisalva T. Guimarães, Ricardo R. dos Santos, Milena B.P. Soares Tags: Original Article Source Type: research

Histological characteristics of the abdominal aortic wall in patients with vascular chronic Q fever
Summary The aim of this study was to describe specific histological findings of the Coxiella burnetii‐infected aneurysmal abdominal aortic wall. Tissue samples of the aneurysmal abdominal aortic wall from seven patients with chronic Q fever and 15 patients without evidence of Q fever infection were analysed and compared. Chronic Q fever was diagnosed using serology and tissue PCR analysis. Histological sections were stained using haematoxylin and eosin staining, Elastica van Gieson staining and immunohistochemical staining for macrophages (CD68), T lymphocytes (CD3), T lymphocyte subsets (CD4 and CD8) and B lymphocytes (...
Source: International Journal of Experimental Pathology - June 1, 2014 Category: Pathology Authors: Julia C. J. P. Hagenaars, Olivier H. J. Koning, Ronald F. F. Haak, Bart A. N. Verhoeven, Nicole H. M. Renders, Mirjam H. A. Hermans, Peter C. Wever, Robert Jan Suylen Tags: Original Article Source Type: research

Reduction of angiotensin A and alamandine vasoactivity in the rabbit model of atherogenesis: differential effects of alamandine and Ang(1‐7)
Summary Novel treatments are necessary to reduce the burden of cardiovascular disease (CVD). Alamandine binds to MrgD and is reported to induce vasodilation via stimulation of endothelial nitric oxide synthase (eNOS), but its role in atherogenic blood vessels is yet to be determined. To determine the vasoactive role of alamandine and its precursor AngA in diseased aorta, New Zealand White rabbits were fed a diet containing 1% methionine + 0.5% cholesterol + 5% peanut oil for 4 weeks (MC, n = 5) or control (n = 6). In abdominal aorta, alamandine (1 μM) was added 30 min before a dose–response curve to angiotens...
Source: International Journal of Experimental Pathology - June 1, 2014 Category: Pathology Authors: Belthrand Habiyakare, Hiba Alsaadon, Michael L. Mathai, Alan Hayes, Anthony Zulli Tags: Original Article Source Type: research

Impact of 5‐azacytidine on rat decidual cell proliferation
Summary The DNA demethylating agent 5‐azacytidine (5‐azaC) has a teratogenic influence during rat development influencing both the embryo and the placenta. Our aim was to investigate its impact on early decidual cell proliferation before the formation of placenta. Thus, female Fischer rats received 5‐azaC (5 mg/kg, i.p.) on the 2nd, 5th or 8th day of gestation and the decidual tissues were harvested on gestation day 9. They were then analysed immunohistochemically for expression of cell proliferation marker proliferating cell nuclear antigen (PCNA) in decidual cells and for global DNA methylation using the coupled r...
Source: International Journal of Experimental Pathology - June 1, 2014 Category: Pathology Authors: Dora Fabijanovic, Alan Serman, Marin Jezic, Ana Katusic, Nino Sincic, Mirna Curkovic‐Perica, Floriana Bulic‐Jakus, Maja Vlahovic, Gordana Juric‐Lekic, Ljiljana Serman Tags: Original Article Source Type: research

Impaired compensatory beta‐cell function and growth in response to high‐fat diet in LDL receptor knockout mice
In this study, we investigated the effect of low density lipoprotein receptor (LDLr) deficiency on gap junctional connexin 36 (Cx36) islet content and on the functional and growth response of pancreatic beta‐cells in C57BL/6 mice fed a high‐fat (HF) diet. After 60 days on regular or HF diet, the metabolic state and morphometric islet parameters of wild‐type (WT) and LDLr−/− mice were assessed. HF diet‐fed WT animals became obese and hypercholesterolaemic as well as hyperglycaemic, hyperinsulinaemic, glucose intolerant and insulin resistant, characterizing them as prediabetic. Also they showed a significant dec...
Source: International Journal of Experimental Pathology - May 24, 2014 Category: Pathology Authors: Ricardo B. d. Oliveira, Carolina P. d. F. Carvalho, Carla C. Polo, Gabriel d. G. Dorighello, Antônio C. Boschero, Helena C. F. d. Oliveira, Carla B. Collares‐Buzato Tags: Original Article Source Type: research

Under pressure: the cell's response
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - May 14, 2014 Category: Pathology Tags: British Society for Matrix Biology – Autumn 2013 Meeting Report Source Type: research

Issue Information
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - May 14, 2014 Category: Pathology Tags: Issue Information Source Type: research

Illustrating the interplay between the extracellular matrix and microRNAs
Summary The discovery of cell surface receptors that bind to extracellular matrix (ECM) components marked a new era in biological research. Since then there has been an increasing appreciation of the importance of studying cells in the context of their extracellular environment. Cell behaviour is profoundly affected by the ECM, whose synthesis and turnover must be finely balanced in order to maintain normal function and prevent disease. In the last decade, microRNAs (miRNAs) have emerged as key regulators of ECM gene expression. As new technologies for the identification and validation of miRNA targets continue to be devel...
Source: International Journal of Experimental Pathology - April 25, 2014 Category: Pathology Authors: Anna M. Piccinini, Kim S. Midwood Tags: Review Article Source Type: research

Evidence for aldosterone‐dependent growth of renal cell carcinoma
Summary The aim if this study was to investigate the hypothesis that K‐RAS 4A is upregulated in a mineralocorticoid‐dependent manner in renal cell carcinoma and that this supports the proliferation and survival of some renal cancers. Expression of the K‐RAS in renal tumour tissues and cell lines was examined by real‐time PCR and Western blot and mineralocorticoid receptor, and its gatekeeper enzyme 11β‐hydroxysteroid dehydrogenase‐2 was examined by immunocytochemistry on a tissue microarray of 27 cases of renal cell carcinoma. Renal cancer cells lines 04A018 (RCC4 plus VHL) and 04A019 (RCC4 plus vector alone) ...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Sharon King, Susan Bray, Sarah Galbraith, Lesley Christie, Stewart Fleming Tags: Original Article Source Type: research

Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprung's disease
In this study, we used two‐dimensional gel electrophoresis (2‐DE) technology coupled with matrix assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) analysis to identify differentially expressed proteins in the aganglionic (stenotic) and ganglionic (normal) colon segment tissues from patients with HSCR. We identified 15 proteins with different expression levels between the stenotic and the normal colon segment tissues from patients with HSCR. Nine proteins were upregulated and six proteins downregulated in the stenotic colon segment tissues compared to the normal colon segment t...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Hong Gao, Xiaomei Liu, Dong Chen, Liangying Lv, Mei Wu, Jie Mi, Weilin Wang Tags: Original Article Source Type: research

A potential link among antioxidant enzymes, histopathology and trace elements in canine visceral leishmaniasis
Summary Canine visceral leishmaniasis (CVL) is a severe and fatal systemic chronic inflammatory disease. We investigated the alterations in, and potential associations among, antioxidant enzymes, trace elements and histopathology in CVL. Blood and tissue levels of Cu‐Zn superoxide dismutase, catalase and glutathione peroxidase were measured in mixed‐breed dogs naturally infected with Leishmania infantum chagasi, symptomatic (n = 19) and asymptomatic (n = 11). Serum levels of copper, iron, zinc, selenium and nitric oxide, and plasma lipid peroxidation were measured. Histological and morphometric analyses were conduc...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Carolina C. Souza, Tatiane de O. Barreto, Sydnei M. Silva, Aldair W. J. Pinto, Maria M. Figueiredo, Olguita G. Ferreira Rocha, Silvia D. Cangussú, Wagner L. Tafuri Tags: Original Article Source Type: research

Duration of injury correlates with necrosis in caerulein‐induced experimental acute pancreatitis: implications for pathophysiology
Summary Pancreatic acinar cell necrosis is indicative of severe pancreatitis and the degree of necrosis is an index of its outcome. We studied whether the dose and duration of injury correlates with severity, particularly in terms of necrosis, in caerulein‐induced acute pancreatitis (AP) in Swiss albino mice. In addition to control group 1 (G1), groups 2 and 3 received four injections of caerulein every hour but were sacrificed at five hours (G2) and nine hours (G3) respectively, and group 4 received eight injections and was sacrificed at nine hours (G4). The severity of pancreatitis was assessed histopathologically and ...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Tony G. Jacob, Rahul Raghav, Ajay Kumar, Pramod K. Garg, Tara S. Roy Tags: Original Article Source Type: research

Frequency and significance of IgG4 immunohistochemical staining in liver explants from patients with primary sclerosing cholangitis
Summary Dense tissue infiltrates of IgG4+ plasma cells >50/high‐powered field (HPF) are purportedly highly specific for IgG4‐related disease. However, the frequency and significance of liver‐infiltrating IgG4+ plasma cells in primary sclerosing cholangitis (PSC) applying these cut‐offs has not been determined. We sought to determine the incidence of intrahepatic IgG4‐positive staining in PSC patients undergoing transplantation, correlating findings with clinical parameters. Immunohistochemical staining was performed on liver explants obtained between 1991 and 2009. Of 122 explants obtained, hilar IgG4+ stainin...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Sandra Fischer, Palak J. Trivedi, Stephen Ward, Paul D. Greig, George Therapondos, Gideon M. Hirschfield Tags: Original Article Source Type: research

Expression profile of undifferentiated cell transcription factor 1 in normal and cancerous human epithelia
Summary Undifferentiated cell Transcription Factor 1 (UTF1) is a chromatin‐bound protein involved in stem cell differentiation. It was initially reported to be restricted to stem cells or germinal tissues. However, recent work suggests that UTF1 is also expressed in somatic cells and that its expression may increase during carcinogenesis. To further clarify the expression profile of UTF1, we evaluated UTF1 expression levels immunohistochemically in eight normal human epithelia (from breast, prostate, endometrium, bladder, colon, oesophagus, lung and kidney) and their corresponding tumours as well as in several epithelial...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Mustapha Mouallif, Adelin Albert, Mustapha Zeddou, My Mustapha Ennaji, Philippe Delvenne, Samuel Guenin Tags: Original Article Source Type: research

Cytokeratin 7/19 expression in N‐diethylnitrosamine‐induced mouse hepatocellular lesions: implications for histogenesis
Summary Hepatocellular carcinoma (HCC) is a common malignancy with poor clinical outcome, whose histogenesis is the subject of intense debate. Specifically, expression of cytokeratins (CKs) 7 and 19, associated with aggressive biological behaviour, is proposed to reflect a possible progenitor cell origin or tumour dedifferentiation towards a primitive phenotype. This work addresses that problem by studying CKs 7 and 19 expression in N‐diethylnitrosamine (DEN)‐induced mouse HCCs. ICR mice were divided into six DEN‐exposed and six matched control groups. Samples were taken from each group at consecutive time points. Hy...
Source: International Journal of Experimental Pathology - April 1, 2014 Category: Pathology Authors: Nuno P. Santos, Paula A. Oliveira, Regina Arantes‐Rodrigues, Ana I. Faustino‐Rocha, Aura Colaço, Carlos Lopes, Rui M. Gil da Costa Tags: Original Article Source Type: research