International Journal of Experimental Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Combined therapy with metformin and insulin attenuates systemic and hepatic alterations in a model of high‐fat diet‐/streptozotocin‐induced diabetes
In this study we have explored the pathogenesis of the hepatic alterations which occur in diabetes and the modulation of these complications by the combination of metformin adjunct treatment and insulin monotherapy. For this purpose, diabetic rats were treated with insulin (DM + Ins) or metformin plus insulin (DM + Met + Ins). Biochemical and cardiometabolic parameters were analysed by spectrophotometry. Intravital microscopy was used to study the hepatic microcirculation. In the liver tissue, real‐time PCR was used to analyse oxidative stress enzymes, inflammatory markers and receptors for advanced glycation end p...
Source: International Journal of Experimental Pathology - July 5, 2016 Category: Pathology Authors: Raquel Rangel Silvares, Evelyn Nunes Goulart da Silva Pereira, Edgar Eduardo Ilaquita Flores, Vanessa Estato, Patrícia Alves Reis, Igor José da Silva, Marcelo Pelajo Machado, Hugo Caire de Castro Faria Neto, Eduardo Tibiriça, Anissa Daliry Tags: Original Article Source Type: research

New approaches in tail ‐bleeding assay in mice: improving an important method for designing new anti‐thrombotic agents
This report describes a modified, simple, low‐cost and more sensitive method to determine bleeding patterns and haemoglobin concentration in a tail‐bleeding assay using BALB/c mice and tail tip amputation. The cut tail was immersed in Drabkin's reagent to promote erythrocyte lysis and haemoglobin release, which was monitored over 30 min. The operator was blinded to individual conditions of the mice, which were treated with either saline (NaCl 0.15m), DMSO (0.5%) or clinical anti‐thrombotic drugs. Our experimental protocols showed good reproducibility and repeatability of results when using Drabkin's reagent than wat...
Source: International Journal of Experimental Pathology - July 4, 2016 Category: Pathology Authors: Max Seidy Saito, Andr é Luiz Lourenço, Hye Chung Kang, Carlos Rangel Rodrigues, Lucio Mendes Cabral, Helena Carla Castro, Plínio Cunha Satlher Tags: Original Article Source Type: research

Issue Information
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - June 28, 2016 Category: Pathology Tags: Issue Information Source Type: research

Age ‐associated decrease in GDNF and its cognate receptor GFRα‐1 protein expression in human skin
Summary Glial cell line‐derived neurotrophic factor (GDNF) and its cognate receptor (GFRα‐1) are expressed in normal human skin. They are involved in murine hair follicle morphogenesis and cycling control. We hypothesize that ‘GDNF and GFRα‐1 protein expression in human skin undergoes age‐associated alterations. To test our hypothesis, the expression of these proteins was examined in human skin specimens obtained from 30 healthy individuals representing three age groups: children (5–18 years), adults (19–60 years) and the elderly (61–81 years). Immunofluorescent and light microscopic immunohistologic a...
Source: International Journal of Experimental Pathology - June 26, 2016 Category: Pathology Authors: Mohamed A. Adly, Hanan A. Assaf, Mahmoud Rezk Abdelwahed Hussein Tags: Original Article Source Type: research

miR ‐376c inhibits cervical cancer cell proliferation and invasion by targeting BMI1
Summary MicroRNAs (miRNAs) play crucial roles in cancer development and progression. The purposes of this study were to explore the role of miR‐376c in cervical cancer and to clarify the regulation of BMI1 by miR‐376c. Quantitative RT‐PCR was used to measure miR‐376c expression in cervical cancer tissues and cell lines. The cell proliferation, cell cycle and Transwell invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR‐376c, and the results were validated in cervical cancer cell lines and tissues. MiR‐376c was significantly downregulated in cervical cancer...
Source: International Journal of Experimental Pathology - June 26, 2016 Category: Pathology Authors: Youping Deng, Yan Xiong, Yingjuan Liu Tags: Original Article Source Type: research

Age‐associated decrease in GDNF and its cognate receptor GFRα‐1 protein expression in human skin
Summary Glial cell line‐derived neurotrophic factor (GDNF) and its cognate receptor (GFRα‐1) are expressed in normal human skin. They are involved in murine hair follicle morphogenesis and cycling control. We hypothesize that ‘GDNF and GFRα‐1 protein expression in human skin undergoes age‐associated alterations. To test our hypothesis, the expression of these proteins was examined in human skin specimens obtained from 30 healthy individuals representing three age groups: children (5–18 years), adults (19–60 years) and the elderly (61–81 years). Immunofluorescent and light microscopic immunohistologic a...
Source: International Journal of Experimental Pathology - June 26, 2016 Category: Pathology Authors: Mohamed A. Adly, Hanan A. Assaf, Mahmoud Rezk Abdelwahed Hussein Tags: Original Article Source Type: research

Loss of p53 expression is accompanied by upregulation of beta ‐catenin in meningiomas: a concomitant reciprocal expression
Summary Crosstalk between Wnt and p53 signalling pathways in cancer has long been suggested. Therefore in this study we have investigated the involvement of these pathways in meningiomas by analysing their main effector molecules, beta‐catenin and p53. Cellular expression of p53 and beta‐catenin proteins and genetic changes in TP53 were analysed by immunohistochemistry, PCR/RFLP and direct sequencing of TP53 exon 4. All the findings were analysed statistically. Our analysis showed that 47.5% of the 59 meningiomas demonstrated loss of expression of p53 protein. Moderate and strong p53 expression in the nuclei was observ...
Source: International Journal of Experimental Pathology - June 11, 2016 Category: Pathology Authors: Nives Pe ćina‐Šlaus, Anja Kafka, Tomislav Vladušić, Davor Tomas, Monika Logara, Josip Skoko, Reno Hrašćan Tags: Original Article Source Type: research

Functional roles of CSPG4/NG2 in chondrosarcoma
Summary CSPG4/NG2 is a multifunctional transmembrane protein with limited distribution in adult tissues including articular cartilage. The purpose of this study was to investigate the possible roles of CSPG4/NG2 in chondrosarcomas and to establish whether this molecule may have potential for targeted therapy. Stable knock‐down of CSPG4/NG2 in the JJ012 chondrosarcoma cell line by shRNA resulted in decreased cell proliferation and migration as well as a decrease in gene expression of the MMP (matrix metalloproteinase) 3 protease and ADAMTS4 (aggrecanase). Chondrosarcoma cells in which CSPG4/NG2 was knocked down were more ...
Source: International Journal of Experimental Pathology - June 11, 2016 Category: Pathology Authors: Nuor S. M. Jamil, Asim Azfer, Harrison Worrel, Donald M. Salter Tags: Original Article Source Type: research

Loss of p53 expression is accompanied by upregulation of beta‐catenin in meningiomas: a concomitant reciprocal expression
Summary Crosstalk between Wnt and p53 signalling pathways in cancer has long been suggested. Therefore in this study we have investigated the involvement of these pathways in meningiomas by analysing their main effector molecules, beta‐catenin and p53. Cellular expression of p53 and beta‐catenin proteins and genetic changes in TP53 were analysed by immunohistochemistry, PCR/RFLP and direct sequencing of TP53 exon 4. All the findings were analysed statistically. Our analysis showed that 47.5% of the 59 meningiomas demonstrated loss of expression of p53 protein. Moderate and strong p53 expression in the nuclei was observ...
Source: International Journal of Experimental Pathology - June 11, 2016 Category: Pathology Authors: Nives Pećina‐Šlaus, Anja Kafka, Tomislav Vladušić, Davor Tomas, Monika Logara, Josip Skoko, Reno Hrašćan Tags: Original Article Source Type: research

Acute paraquat exposure determines dose ‐dependent oxidative injury of multiple organs and metabolic dysfunction in rats: impact on exercise tolerance
This study investigated the pathological morphofunctional adaptations related to the imbalance of exercise tolerance triggered by paraquat (PQ) exposure in rats. The rats were randomized into four groups with eight animals each: (a) SAL (control): 0.5 ml of 0.9% NaCl solution; (b) PQ10: PQ 10 mg/kg; (c) PQ20: PQ 20 mg/kg; and (d) PQ30: PQ 30 mg/kg. Each group received a single injection of PQ. After 72 hours, the animals were subjected to an incremental aerobic running test until fatigue in order to determine exercise tolerance, blood glucose and lactate levels. After the next 24 h, lung, liver and skeletal muscle we...
Source: International Journal of Experimental Pathology - June 8, 2016 Category: Pathology Authors: R ômulo D. Novaes, Reggiani V. Gonçalves, Marli C. Cupertino, Eliziária C. Santos, Solange M. Bigonha, Geraldo J. M. Fernandes, Izabel R. S. C. Maldonado, Antônio J. Natali Tags: Original Article Source Type: research

Acute paraquat exposure determines dose‐dependent oxidative injury of multiple organs and metabolic dysfunction in rats: impact on exercise tolerance
This study investigated the pathological morphofunctional adaptations related to the imbalance of exercise tolerance triggered by paraquat (PQ) exposure in rats. The rats were randomized into four groups with eight animals each: (a) SAL (control): 0.5 ml of 0.9% NaCl solution; (b) PQ10: PQ 10 mg/kg; (c) PQ20: PQ 20 mg/kg; and (d) PQ30: PQ 30 mg/kg. Each group received a single injection of PQ. After 72 hours, the animals were subjected to an incremental aerobic running test until fatigue in order to determine exercise tolerance, blood glucose and lactate levels. After the next 24 h, lung, liver and skeletal muscle we...
Source: International Journal of Experimental Pathology - June 8, 2016 Category: Pathology Authors: Rômulo D. Novaes, Reggiani V. Gonçalves, Marli C. Cupertino, Eliziária C. Santos, Solange M. Bigonha, Geraldo J. M. Fernandes, Izabel R. S. C. Maldonado, Antônio J. Natali Tags: Original Article Source Type: research

Protocol for HER2 FISH determination on PAXgene ‐fixed and paraffin‐embedded tissue in breast cancer
In this study, we have developed and tested a protocol for PAXgene‐fixed and paraffin‐embedded tissues for fluorescent in situ hybridization (FISH). The implementation of a 24‐h formalin postfixation step of slides from PAXgene‐fixed and paraffin‐embedded tissues allowed us to use the assays approved for formalin‐fixed and paraffin‐embedded tissues. The equivalence of the methodologies was demonstrated by FISH analysis of HER2 amplification in breast cancer cases. The 24‐h postfixation step of the slides used for FISH can be well integrated in the routine diagnostic workflow and allows the remaining PAXgen...
Source: International Journal of Experimental Pathology - June 7, 2016 Category: Pathology Authors: Lisa Oberauner ‐Wappis, Martina Loibner, Christian Viertler, Daniel Groelz, Ralf Wyrich, Kurt Zatloukal Tags: Original Article Source Type: research

A high ‐throughput assay for the measurement of uropathogenic Escherichia coli attachment to urinary bladder cells
Summary Strains of uropathogenic Escherichia coli (UPEC) are the major causative agent of urinary tract infections (UTI), the most common infectious diseases in the world. Their ability to attach and enter into cells in the urinary tract is a limiting step for their pathogenicity. Many studies are thus focussing on these key mechanisms to propose new therapeutic strategies. To facilitate such studies, we developed a fast and high‐throughput assay which makes it possible to monitor the interaction of UPEC with cultured human uroepithelial cells. This assay allows measurement of the in vitro association of fluorescently l...
Source: International Journal of Experimental Pathology - June 7, 2016 Category: Pathology Authors: Karellen Beren Garc ía Méndez, Gabriel Bragagnolo, David O'Callaghan, Jean‐Philippe Lavigne, Anne Keriel Tags: Original Article Source Type: research

Effect of in utero exposure to the atypical anti ‐psychotic risperidone on histopathological features of the rat placenta
This study revealed that in utero exposure to equivalent therapeutic doses of RIS during organogenesis‐induced placental dystrophy (size and weight), disturbed cytoarchitectural organization (thickness of different placental layers), histopathological lesions (necrosis in trophoblast with disruption of trophoblastic septa and rupturing of maternal‐foetal interface) and intrauterine growth restriction of the foetuses. It may be concluded that multifactorial mechanisms might be involved in the dysregulation of structure and function of the placenta and of poor foetal growth and development. (Source: International Journal...
Source: International Journal of Experimental Pathology - June 2, 2016 Category: Pathology Authors: K.P. Singh, Manoj K. Singh, Shrikant Gautam Tags: Original Article Source Type: research

Effect of in utero exposure to the atypical anti‐psychotic risperidone on histopathological features of the rat placenta
This study revealed that in utero exposure to equivalent therapeutic doses of RIS during organogenesis‐induced placental dystrophy (size and weight), disturbed cytoarchitectural organization (thickness of different placental layers), histopathological lesions (necrosis in trophoblast with disruption of trophoblastic septa and rupturing of maternal‐foetal interface) and intrauterine growth restriction of the foetuses. It may be concluded that multifactorial mechanisms might be involved in the dysregulation of structure and function of the placenta and of poor foetal growth and development. (Source: International Journal...
Source: International Journal of Experimental Pathology - June 2, 2016 Category: Pathology Authors: K.P. Singh, Manoj K. Singh, Shrikant Gautam Tags: Original Article Source Type: research