International Journal of Experimental Pathology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

British Society for Matrix Biology – Spring 2015 Meeting Report
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - February 7, 2016 Category: Pathology Tags: Abstracts Source Type: research

Issue Information
(Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - February 7, 2016 Category: Pathology Tags: Issue Information Source Type: research

miR‐376c inhibits cervical cancer cell proliferation and invasion by targeting BMI1
Summary MicroRNAs (miRNAs) play crucial roles in cancer development and progression. The purposes of this study were to explore the role of miR‐376c in cervical cancer and to clarify the regulation of BMI1 by miR‐376c. Quantitative RT‐PCR was used to measure miR‐376c expression in cervical cancer tissues and cell lines. The cell proliferation, cell cycle and Transwell invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR‐376c, and the results were validated in cervical cancer cell lines and tissues. MiR‐376c was significantly downregulated in cervical cancer...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Youping Deng, Yan Xiong, Yingjuan Liu Tags: Original Article Source Type: research

Correlation between liver cell necrosis and circulating alanine aminotransferase after ischaemia/reperfusion injuries in the rat liver
Summary Circulating liver enzymes such as alanine transaminase are often used as markers of hepatocellular damage. Ischaemia/reperfusion (I/R) injury is an inevitable consequence of prolonged liver ischaemia. The aim of this study was to examine the correlation between liver enzymes and volume of liver cell necrosis after ischaemia/reperfusion injuries, using design‐unbiased stereological methods. Forty‐seven male Wistar rats were subjected to 1 h of partial liver ischaemia, followed by either 4 or 24 h of reperfusion. Within each group, one‐third of animals were subjected to ischaemic preconditioning and one‐thi...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Anders R. Knudsen, Kasper J. Andersen, Stephen Hamilton‐Dutoit, Jens R. Nyengaard, Frank V. Mortensen Tags: Original Article Source Type: research

Expression of FOXM1 and related proteins in breast cancer molecular subtypes
In conclusion, although high FOXM1 expression was noted in the TNBC subtype, it had no prognostic impact in TNBC. However, it had prognostic significance in the hormone receptor‐positive/HER2‐negative subtype. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Jeong‐Ju Lee, Hee Jin Lee, Byung‐Ho Son, Sung‐Bae Kim, Jin‐Hee Ahn, Seung Do Ahn, Eun Yoon Cho, Gyungyub Gong Tags: Original Article Source Type: research

Protocol for HER2 FISH determination on PAXgene‐fixed and paraffin‐embedded tissue in breast cancer
In this study, we have developed and tested a protocol for PAXgene‐fixed and paraffin‐embedded tissues for fluorescent in situ hybridization (FISH). The implementation of a 24‐h formalin postfixation step of slides from PAXgene‐fixed and paraffin‐embedded tissues allowed us to use the assays approved for formalin‐fixed and paraffin‐embedded tissues. The equivalence of the methodologies was demonstrated by FISH analysis of HER2 amplification in breast cancer cases. The 24‐h postfixation step of the slides used for FISH can be well integrated in the routine diagnostic workflow and allows the remaining PAXgen...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Lisa Oberauner‐Wappis, Martina Loibner, Christian Viertler, Daniel Groelz, Ralf Wyrich, Kurt Zatloukal Tags: Original Article Source Type: research

A high‐throughput assay for the measurement of uropathogenic Escherichia coli attachment to urinary bladder cells
Summary Strains of uropathogenic Escherichia coli (UPEC) are the major causative agent of urinary tract infections (UTI), the most common infectious diseases in the world. Their ability to attach and enter into cells in the urinary tract is a limiting step for their pathogenicity. Many studies are thus focussing on these key mechanisms to propose new therapeutic strategies. To facilitate such studies, we developed a fast and high‐throughput assay which makes it possible to monitor the interaction of UPEC with cultured human uroepithelial cells. This assay allows measurement of the in vitro association of fluorescently l...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Karellen Beren García Méndez, Gabriel Bragagnolo, David O'Callaghan, Jean‐Philippe Lavigne, Anne Keriel Tags: Original Article Source Type: research

Establishing a rat model for the study of vitamin K deficiency
In conclusion, the combination of a vitamin K‐deficient diet with the administration of gentamicin results in a useful model of vitamin K‐deficieny. (Source: International Journal of Experimental Pathology)
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Yanni Mi, Xue Xiao, Dongzheng Liu, Nana Ping, Yanbing Zhu, Bo Li, Lihui Long, Yongxiao Cao Tags: Original Article Source Type: research

Immunohistochemical study of hepatic fibropoiesis associated with canine visceral leishmaniasis
Summary Hepatic fibropoiesis has been confirmed in canine visceral leishmaniasis. In fibrotic disease, hepatic stellate cells (HSC) play an important role in fibropoiesis, undergoing activation by TGF‐β to acquire characteristics of myofibroblasts. These cells show extensive capacity for proliferation, motility, contractility, collagen synthesis and extracellular matrix component synthesis. The aim of this work was to identify markers of HSC activation in 10 symptomatic and 10 asymptomatic dogs naturally infected with Leishmania (Leishmania) infantum. Eight uninfected dogs were used as controls. Alpha‐actin (α‐SMA)...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Igor M. V. M. Madeira, Debora. M. O. Pereira, Aline. A. Sousa, Cesar A. Vilela, Izabela F. G. Amorim, Marcelo V. Caliari, Carolina C. Souza, Wagner L. Tafuri Tags: Original Article Source Type: research

A 12‐month follow‐up study on the preventive effect of oral lansoprazole on acute exacerbation of chronic obstructive pulmonary disease
The objective of this study was to evaluate the preventive effects of oral administration of lansoprazole on acute exacerbation of chronic obstructive pulmonary disease (COPD). Patients with COPD in groups C and D in the stable phase were stratified into a group with neither gastroesophageal reflux nor lansoprazole therapy (group A) and a group subjected to oral lansoprazole therapy (group B1) and a group not subjected to oral lansoprazole therapy (group B2). The frequency scale for the symptoms of gastroesophageal reflux disease (FSSG) questionnaire, COPD assessment test (CAT) questionnaire, pulmonary function test and th...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Wei Xiong, Quan‐san Zhang, Wei Zhao, Wei Ding, Jin‐ming Liu, Yun‐feng Zhao Tags: Original Article Source Type: research

Overexpression of trophoblast cell surface antigen 2 as an independent marker for a poor prognosis and as a potential therapeutic target in epithelial ovarian carcinoma
In this study, we further evaluated whether TROP2 is a promising marker for EOC, and thus also a potential target for EOC immunotherapy. Quantitative real‐time polymerase chain reaction (qPCR) and fluorescence‐activated cell sorting (FACS) analysis were employed to determine TROP2 mRNA and protein expression in both human EOC and normal ovarian cell lines. Additionally, TROP2 protein expression was measured by immunohistochemistry in 128 EOC tissue samples, 21 normal ovarian tissues and 18 normal fallopian tubes. The correlations between TROP2 protein expression and patients' clinicopathological features were investig...
Source: International Journal of Experimental Pathology - January 31, 2016 Category: Pathology Authors: Ning Xu, Zhihong Zhang, Jin Zhu, Lu Xu, Yuhua Li, Lei Duan, Yuan Mao, Hongxia Li Tags: Original Article Source Type: research

Multiple pathways regulate Cten in colorectal cancer without a Tensin switch
In this study, we investigated the roles of EGFR and STAT3 signalling in the regulation of Cten in colorectal cancer (CRC). In addition, we investigated calpain – a regulator of focal adhesion‐associated proteins whose relevance to Cten has not been investigated. CRC cell lines were stimulated with epidermal growth factor (EGF). This resulted in an increase in Cten and Tensin 3 protein. Kras was knocked down and this resulted in downregulation of Cten and Tensin 3. We next investigated the role of STAT3 signalling. Activation and knockdown of STAT3 resulted in downregulation and upregulation, respectively, of Cten. Inh...
Source: International Journal of Experimental Pathology - January 19, 2016 Category: Pathology Authors: Hannah Thorpe, Maham Akhlaq, Darryl Jackson, Saleh Al Ghamdi, Sarah Storr, Stewart Martin, Mohammad Ilyas Tags: Original Article Source Type: research

Recombinant human brain natriuretic peptide attenuates trauma‐/haemorrhagic shock‐induced acute lung injury through inhibiting oxidative stress and the NF‐κB‐dependent inflammatory/MMP‐9 pathway
In this study, we investigated local changes in oxidative stress and the NF‐κB‐dependent matrix metalloproteinase‐9 (MMP‐9) pathway in rats with trauma/haemorrhagic shock (TH/S)‐induced ALI and evaluated the effects of pretreatment with rhBNP. Forty‐eight rats were randomly divided into four groups: sham operation group, model group, low‐dosage rhBNP group and high‐dosage rhBNP group (n = 12 for each group). Oxidative stress and MPO activity were measured by ELISA kits. MMP‐9 activity was detected by zymography analysis. NF‐κB activity was determined using Western blot assay. With rhBNP pretreatment...
Source: International Journal of Experimental Pathology - January 19, 2016 Category: Pathology Authors: Zhi Song, Xiu Zhao, Martin Liu, Hongxu Jin, Ling Wang, Mingxiao Hou, Yan Gao Tags: Original Article Source Type: research

Treatment with Mesna and n‐3 polyunsaturated fatty acids ameliorates experimental ulcerative colitis in rats
Summary Oxidative damage is a central feature of ulcerative colitis. Here, we tested whether the antioxidant Mesna, when administered alone or in combination with n‐3 polyunsaturated fatty acids (n‐3 PUFAs), affects the outcome of dextran sodium sulphate (DSS)‐induced ulcerative colitis in rats. After the induction of colitis, DSS‐treated rats were further treated orally (p.o), intraperitoneally (i.p) or intrarectally (i.r) for either 7 or 14 days with Mesna, n‐3 PUFAs or both. Rats were euthanized at the end of each treatment period. Clinical disease activity index was recorded throughout the experiment. At nec...
Source: International Journal of Experimental Pathology - January 19, 2016 Category: Pathology Authors: Ioannis Triantafyllidis, Theofilos Poutahidis, Ioannis Taitzoglou, Isaak Kesisoglou, Charalampos Lazaridis, Dimitrios Botsios Tags: Original Article Source Type: research

Characterization of lesion formation in marmosets following inhalational challenge with different strains of Burkholderia pseudomallei
Summary The marmoset model of melioidosis was used to explore whether there was any difference in the disease presentation and/or the lesion formation following inhalational challenge with one of four strains of Burkholderia pseudomallei (K96243, 1026b, HBPUB10303a and HBPUB10134a). Marmosets were challenged with a range of bacterial doses and bacterial load, histological and physiological features were determined temporally following lethal disease. Melioidosis presented as an acute, febrile disease with bacteraemia, bacterial dissemination, necrotizing hepatitis, splenitis and pneumonia which was independent of the chall...
Source: International Journal of Experimental Pathology - January 19, 2016 Category: Pathology Authors: Michelle Nelson, Alejandro Nunez, Sarah A. Ngugi, Adam Sinclair, Timothy P. Atkins Tags: Original Article Source Type: research