Protective effect of mirtazapine and hesperidin on cyclophosphamide-induced oxidative damage and infertility in rat ovaries
Cyclophosphamide (CP) causes infertility due to ovarian toxicity. The toxicity mechanism suggests oxidative stress. We assessed whether mirtazapine (MTZ) and hesperidin (HSP) could promote ovarian protection against damage due to CP chemotherapy. Female Wistar rats aged 14 weeks were used. Animals were divided into four groups: control vehicle group (n = 8); CP group (n = 8, rats received 150 mg/kg of CP, single intraperitoneal [i.p.] injection); CP + MTZ group (n = 8, rats received same dose of CP + 30 mg/kg of MTZ, orally, daily); and HSP + CP group (n = 8, rats received same dose of CP + 100 mg/kg of HSP, orally, daily)...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Khedr, N. F. Tags: Pharmacology/Toxicology Source Type: research
Resveratrol synergistically augments anti-tumor effect of 5-FU in vitro and in vivo by increasing S-phase arrest and tumor apoptosis
In conclusion, the 5-FU/Res combination enabled a more effective inhibition of cell growth and the induction of apoptosis in cancer cells than 5-FU alone. The results of this study suggest that chemotherapy using natural dietary agents with chemical agents represents a superior cancer treatment option. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Dun, J., Chen, X., Gao, H., Zhang, Y., Zhang, H., Zhang, Y. Tags: Pharmacology/Toxicology Source Type: research
Roxithromycin inhibits nuclear factor kappaB signaling and endoplasmic reticulum stress in intestinal epithelial cells and ameliorates experimental colitis in mice
Roxithromycin is known to have anti-inflammatory and immunoregulatory activity. However, little information is available on the effect of roxithromycin in intestinal inflammation. The aim of this study was to investigate the effect of roxithromycin on NF– B signaling and ER stress in intestinal epithelial cells (IECs) and the effect of roxithromycin on dextran sulfate sodium (DSS)-induced acute colitis in a murine model. HCT116 cells and COLO205 cells were pretreated with roxithromycin and then stimulated with tumor necrosis factor-α (TNF-α). Interleukin (IL)-8 expression was determined by real-time rever...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Choi, Y., Koh, S.-J., Lee, H. S., Kim, J. W., Gwan Kim, B., Lee, K. L., Kim, J. S. Tags: Immunology/Microbiology/Virology Source Type: research
Bee venom suppresses testosterone-induced benign prostatic hyperplasia by regulating the inflammatory response and apoptosis
Benign prostatic hyperplasia (BPH), which is a common disorder in aging men, involves inflammation that is associated with an imbalance between cell proliferation and cell death. Because current BPH drug treatments have undesirable side effects, the development of well-tolerated and effective alternative medicines to treat BPH is of interest. Bee venom (BV) has been used in traditional medicine to treat conditions, such as arthritis and rheumatism, and pain. Although inflammation has been associated with BPH and BV has strong anti-inflammatory effects, the effects of BV on BPH are not fully understood. Therefore, in this s...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Chung, K.-S., An, H.-J., Cheon, S.-Y., Kwon, K.-R., Lee, K.-H. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
Anti-inflammatory and wound healing potential of cashew apple juice (Anacardium occidentale L.) in mice
Cashew apple is a tropical pseudofruit consumed as juice due to its excellent nutritional and sensory properties. In spite of being well known for its important antioxidant properties, the cashew apple has not been thoroughly investigated for its therapeutic potential. Thereby, this study evaluated the antioxidant capacity, anti-inflammatory, and wound-healing activities of cashew apple juice. Juices from ripe and immature cashew apples were analyzed for antioxidant, anti-inflammatory, and wound-healing properties. Those were evaluated in murine models of xylene-induced ear edema and wound excision. Swiss mice were treated...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: da Silveira Vasconcelos, M., Gomes-Rochette, N. F., de Oliveira, M. L. M., Nunes-Pinheiro, D. C. S., Tome, A. R., Maia de Sousa, F. Y., Pinheiro, F. G. M., Moura, C. F. H., Miranda, M. R. A., Mota, E. F., de Melo, D. F. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
Antitumor efficacy and intratumoral distribution of SN-38 from polymeric depots in brain tumor model
We investigate antitumor efficacy and 2D and 3D intratumoral distribution of 7-ethyl-10-hydroxycamptothecin (SN-38) from polymeric depots inside U-87MG xenograft tumor model in nude mice. Results showed that polymeric depots could be used to administer and controlled release of a large amount of SN-38 directly to the brain tumor model. SN-38 released from depots suppressed tumor growth, where the extent of suppression greatly depended on doses and the number of depot injections. Tumor suppression of SN-38 from depots was three-fold higher in animals which received double injections of depots at high dose (9.7 mg of SN-38) ...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Vejjasilpa, K., Nasongkla, N., Manaspon, C., Larbcharoensub, N., Boongird, A., Hongeng, S., Israsena, N. Tags: Biomedical Engineering Source Type: research
Human miR-3145 inhibits influenza A viruses replication by targeting and silencing viral PB1 gene
In conclusion, this study demonstrated that human miR-3145 triggered silencing of viral PB1 genes and lead to inhibition of multiple subtypes of influenza viral replication. Therefore, hsa-miR-3145 might be useful for alternative treatment of influenza A viruses in the future. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Khongnomnan, K., Makkoch, J., Poomipak, W., Poovorawan, Y., Payungporn, S. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Interleukin-10 gene-carrying bifidobacteria ameliorate murine ulcerative colitis by regulating regulatory T cell/T helper 17 cell pathway
In conclusion, oral administration of BL-hIL-10 can alleviate the inflammation responses of UC in murine model through the restoration of Treg/Th17 imbalance, which might be at least partially due to the inhibition of hypoxia–mTOR–HIF-1α–Th17 axis as well as IL-6–STAT3–HIF-1α–Th17 pathway. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Zhang, D., Wei, C., Yao, J., Cai, X., Wang, L. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Ginsenoside Rb1 inhibits matrix metalloproteinase 13 through down-regulating Notch signaling pathway in osteoarthritis
In conclusion, GRb1 inhibits MMP-13 through down-regulating Notch signaling pathway in OA. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Wang, W., Zeng, L., Wang, Z.-m., Zhang, S., Rong, X.-F., Li, R.-H. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
RTEF-1 protects against oxidative damage induced by H2O2 in human umbilical vein endothelial cells through Klotho activation
In this study, we found that overexpressing RTEF-1 in H2O2-treated human umbilical vein endothelial cells decreased senescence-associated-β-galactosidase (SA-β-gal)-positive cells and G0/G1 cells population. The expressions of p53 and p21 were decreased in H2O2-treated RTEF-1 o/e human umbilical vein endothelial cells. However, specific small interfering RNA of RTEF-1 totally reversed the anti-oxidation effect of RTEF-1 and inhibited RTEF-1-induced decreased p53 and p21 expressions. It demonstrated that RTEF-1 could protect cells from H2O2-induced oxidative damage. In addition, we demonstrated that RTEF-1 could u...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Sun, S., Cheng, B., Sun, P.-G., Wu, X.-H., Wu, Q.-Q., He, P. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Expression and promoter methylation status of hMLH1, MGMT, APC, and CDH1 genes in patients with colon adenocarcinoma
Colorectal cancer (CRC) is the third most common cancer in men and the second in women worldwide. CRC development is the result of genetic and epigenetic alterations accumulation in the epithelial cells of colon mucosa. In the present study, DNA methylation, an epigenetic event, was evaluated in tumoral and matching normal epithelium in a cohort of 61 CRC patients. The results confirmed and expanded knowledge for the tumor suppressor genes hMLH1, MGMT, APC, and CDH1. Promoter methylation was observed for all the examined genes in different percentage. A total of 71% and 10% of the examined cases were found to be methylated...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Michailidi, C., Theocharis, S., Tsourouflis, G., Pletsa, V., Kouraklis, G., Patsouris, E., Papavassiliou, A. G., Troungos, C. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Autophagy activation attenuates renal ischemia-reperfusion injury in rats
This study investigated the role of autophagy in renal I/R injury and explored potential mechanisms in an established rat renal I/R injury model. Forty male Wistar rats were randomly divided into four groups: Sham, I/R, I/R pretreated with 3-methyladenine (3-MA, autophagy inhibitor), or I/R pretreated with rapamycin (autophagy activator). All rats were subjected to clamping of the left renal pedicle for 45 min after right nephrectomy, followed by 24 h of reperfusion. The Sham group underwent the surgical procedure without ischemia. 3-MA and rapamycin were injected 15 min before ischemia. Renal function was indicated by blo...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Zhang, Y.-L., Zhang, J., Cui, L.-Y., Yang, S. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Featured Article: microRNA-125a in pulmonary hypertension: Regulator of a proliferative phenotype of endothelial cells
Vascular remodeling due to excessive proliferation of endothelial and smooth muscle cells is a hallmark feature of pulmonary hypertension. microRNAs (miRNAs) are a class of small, non-coding RNA fragments that have recently been associated with remodeling of pulmonary arteries, in particular by silencing the bone morphogenetic protein receptor type II (BMPR2). Here we identified a novel pathway involving the concerted action of miR-125a, BMPR2 and cyclin-dependent kinase inhibitors (CDKN) that controls a proliferative phenotype of endothelial cells. An in silico approach predicted miR-125a to target BMPR2. Functional inhib...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Huber, L. C., Ulrich, S., Leuenberger, C., Gassmann, M., Vogel, J., von Blotzheim, L. G., Speich, R., Kohler, M., Brock, M. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Renoprotective effects of angiotensin receptor blocker and stem cells in acute kidney injury: Involvement of inflammatory and apoptotic markers
Cisplatin, Cis-diamminedichloroplatinum (CDDP), is a platinum-based chemotherapy drug, and its chemotherapeutic use is restricted by nephrotoxicity. Inflammatory and apoptotic mechanisms play a central role in the pathogenesis of CDDP-induced acute kidney injury (AKI). The aim of this study was to compare the therapeutic potential of candesartan, angiotensin II receptor blocker, versus bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model of CDDP-induced nephrotoxicity. Adult male Wistar rats (n = 40) were divided into four groups; Normal control: received saline injection, CDPP group: received CDDP injection...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Sherif, I. O., Al-Mutabagani, L. A., Alnakhli, A. M., Sobh, M. A., Mohammed, H. E. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Angiotensin II increases matrix metalloproteinase 2 expression in human aortic smooth muscle cells via AT1R and ERK1/2
In conclusion, these results indicate that Ang II can increase the expression of MMP-2 via AT1 receptor and ERK1/2 signaling pathways in human ASMCs and suggest that antagonists of AT1R and ERK1/2 may be useful for treating TAAs. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Wang, C., Qian, X., Sun, X., Chang, Q. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
