Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections
We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Chen, Y.-S., Li, H.-R., Zhang, W., Hua, Z.-D., Lin, X.-H., Lin, M.-Q., Huang, W.-S., Huang, L.-P., Yu, X.-L., Xu, N.-L., Lin, M., Xie, B.-S., Shen, X.-N., Xie, J.-F., Wang, Y., Huang, M., Wu, Y.-A., Hu, X.-L. Tags: Immunology, Microbiology, Virology Source Type: research
Repeatedly administered antidepressant drugs modulate humoral and cellular immune response in mice through action on macrophages
Depression is associated with an altered immune response, which could be normalized by antidepressant drugs. However, little is known about the influence of antidepressants on the peripheral immune response and function of macrophages in individuals not suffering from depression. Our studies were aimed at determining the influence of antidepressant drugs on the humoral and cellular immune response in mice. Mice were treated intraperitoneally with imipramine, fluoxetine, venlafaxine, or moclobemide and contact immunized with trinitrophenyl hapten followed by elicitation and measurement of contact sensitivity by ear swelling...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Nazimek, K., Kozlowski, M., Bryniarski, P., Strobel, S., Bryk, A., Myszka, M., Tyszka, A., Kuszmiersz, P., Nowakowski, J., Filipczak-Bryniarska, I. Tags: Immunology, Microbiology, Virology Source Type: research
Promoter methylation status of tumor suppressor genes and inhibition of expression of DNA methyltransferase 1 in non-small cell lung cancer
DNA methylation is an epigenetic DNA modification catalyzed by DNA methyltransferase 1 (DNMT1). The purpose of this study was to investigate DNMT1 gene and protein expression and the effects of methylation status on tumor suppressor genes in human non-small cell lung cancer (NSCLC) cell lines grown in vitro and in vivo. Human lung adenocarcinoma cell lines, A549 and H838, were grown in vitro and inoculated subcutaneously into nude mice to form tumors and were then treated with the DNA methylation inhibitor, 5-aza-2'-deoxycytidine, with and without treatment with the benzamide histone deacetylase inhibitor, e...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Liu, B., Song, J., Luan, J., Sun, X., Bai, J., Wang, H., Li, A., Zhang, L., Feng, X., Du, Z. Tags: Cell & amp;amp; Developmental Biology Source Type: research
Gene-gene interaction of erythropoietin gene polymorphisms and diabetic retinopathy in Chinese Han
In conclusion, our results support an important association of rs507392, rs1617640 and rs551238 minor allele of erythropoietin with increased diabetic retinopathy risk, and additional interaction among three single nucleotide polymorphisms. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Fan, Y., Fu, Y.-Y., Chen, Z., Hu, Y.-Y., Shen, J. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Benzopyrene promotes lung cancer A549 cell migration and invasion through up-regulating cytokine IL8 and chemokines CCL2 and CCL3 expression
In this study, lung carcinoma A549 cell line was used to investigate the potential roles of tobacco-sourced B[a]P on cell metastasis and invasion and to assess its underlying mechanism. Effects of tobacco-sourced carcinogen on A549 cell proliferation, metastasis, and invasion were analyzed using MTT assay, Transwell assay, and scratch method, respectively. The effects of tobacco-sourced carcinogen on cytokines and chemokines secretion were detected using enzyme-linked immunosorbent assay. Moreover, correlation between inflammatory factor expression and cancer cell migration and invasion was assessed using siRNA-mediated ge...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Zhang, J., Chang, L., Jin, H., Xia, Y., Wang, L., He, W., Li, W., Chen, H. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Exogenous spermine inhibits hypoxia/ischemia-induced myocardial apoptosis via regulation of mitochondrial permeability transition pore and associated pathways
In conclusion, H/I decreased endogenous spermine concentrations in cardiomyocytes, which ultimately induced apoptosis. The addition of exogenous spermine effectively prevented myocyte cell death. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Wei, C., Li, H., Wang, Y., Peng, X., Shao, H., Li, H., Bai, S., Xu, C. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Toll-like receptor 9 regulates melanogenesis through NF-{kappa}B activation
Toll-like receptors play essential roles in the modulation of melanogenesis, which has been implicated in the pathogenesis of hyper- or hypopigmentation-related diseases. However, little is currently known regarding the role of TLR9 in human melanocytes. TLR9 recognizes unmethylated cytosine-phosphate-guanine motif-containing oligodeoxynucleotides, and cytosine-phosphate-guanine ODN2006 acts as an hTLR9 agonist. The aim of the present study was to investigate the effect of cytosine-phosphate-guanine ODN2006 on melanogenesis in the human melanocyte cells. MTT assay and enzyme-linked immunosorbent assay indicated that ODN200...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Sun, L., Pan, S., Yang, Y., Sun, J., Liang, D., Wang, X., Xie, X., Hu, J. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
The common variant Q192R at the paraoxonase 1 (PON1) gene and its activity are responsible for a portion of the altered antioxidant status in type 2 diabetes
In this study, we investigated the effects of paraoxonase 1 (PON1) activities and the variant PON1–Q192R on the ferric reducing ability of plasma (FRAP) and total thiol. In addition, we examined the distribution of genotypes of this variant and the relationship of the genotypes with age in patients with type 2 diabetes (T2D). A total of 115 patients with T2D were enrolled in this study. Paraoxonase activity (PON-para) and arylesterase activity (PON-aryl) were determined using spectrophotometric assays. The distribution of the Q192R genotypes was determined by the double substrate method. The antioxidant status was ev...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Zargari, M., Sharafeddin, F., Mahrooz, A., Alizadeh, A., Masoumi, P. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Featured Article: Effect of copper on nuclear translocation of copper chaperone for superoxide dismutase-1
This study thus demonstrates that under hypoxia condition, both copper and CCS-1 are transported to the nucleus. The nuclear translocation of CCS-1 is copper dependent, but the nuclear translocation of copper could take place alternatively in a CCS-1-independent pathway. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Wang, L., Ge, Y., Kang, Y. J. Tags: IEBMC papers Source Type: research
Post-translational modification profiling - A novel tool for mapping the protein modification landscape in cancer
The ubiquitin system plays an important role in essentially every cellular process, regulating numerous pathways ranging from development, transcription, DNA damage response, cell cycle, and signal transduction. Its best studied role involves removal of faulty proteins or those that are not necessary anymore. Aberrations in the ubiquitin system have been implicated in various pathologies including cancer, where specific mutations in E3 ligases such as Mdm2, pVHL, and BRCA1 have been linked to disease progression, prognosis, and resistance to drugs. Yet, there are hundreds of E3 ligases in the human genome and our knowledge...
Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Eisenberg-Lerner, A., Ciechanover, A., Merbl, Y. Tags: IEBMC papers Source Type: research
Erratum
(Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 19, 2016 Category: Research Tags: Erratum Source Type: research
Phospholipid transfer protein deficiency in mice impairs macrophage reverse cholesterol transport in vivo
Phospholipid transfer protein is expressed in various cell types and secreted into plasma, where it transfers phospholipids between lipoproteins and modulates the composition of high-density lipoprotein particles. Phospholipid transfer protein deficiency in vivo can lower high-density lipoprotein cholesterol level significantly and impact the biological quality of high-density lipoprotein. Considering high-density lipoprotein was a critical determinant for reverse cholesterol transport, we investigated the role of systemic phospholipid transfer protein deficiency in macrophage reverse cholesterol transport in viv...
Source: Experimental Biology and Medicine - July 7, 2016 Category: Research Authors: Si, Y., Zhang, Y., Chen, X., Zhai, L., Zhou, G., Yu, A., Cao, H., Shucun, Q. Tags: Physiology Source Type: research
Cytotoxicity of atropine to human corneal endothelial cells by inducing mitochondrion-dependent apoptosis
In conclusion, atropine above 1/128 of its clinical therapeutic dosage has a dose- and time-dependent cytotoxicity to HCE cells in vitro which is confirmed by CCE cells in vivo, and its cytotoxicity is achieved by inducing HCE cell apoptosis via a death receptor-mediated mitochondrion-dependent signaling pathway. Our findings provide new insights into the cytotoxicity and apoptosis-inducing effect of atropine which should be used with great caution in eye clinic. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 7, 2016 Category: Research Authors: Wen, Q., Fan, T.-J., Tian, C.-L. Tags: Pharmacology & amp;amp; Toxicology Source Type: research
Detection of expressional changes induced by intrauterine growth restriction in the developing rat pancreas
In conclusion, our data support the hypothesis that IUGR influences the development of the rat pancreas. We also identified new pathways that appear to be programmed by IUGR. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - July 7, 2016 Category: Research Authors: Zhang, L., Chen, W., Dai, Y., Zhu, Z., Liu, Q. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
Advanced glycation end-products and insulin signaling in granulosa cells
Advanced glycation end-products (AGEs) may interfere with insulin intracellular signaling and glucose transport in human granulosa cells, potentially affecting ovarian function, follicular growth, linked with diminished fertility. The potential interaction of AGEs with insulin signaling pathways and glucose transport was investigated in human granulosa KGN cells. KGN cells were cultured with variable concentrations of human glycated albumin (HGA, 50–200 µg/mL) or insulin (100 ng/mL). Combined treatments of KGN cells with insulin (100 ng/mL) and HGA (200 µg/mL) were also performed. p-AKT levels and glucose...
Source: Experimental Biology and Medicine - July 7, 2016 Category: Research Authors: Diamanti-Kandarakis, E., Chatzigeorgiou, A., Papageorgiou, E., Koundouras, D., Koutsilieris, M. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
