Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections

We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.

Source: Experimental Biology and Medicine - July 28, 2016 Category: Research Authors: Chen, Y.-S., Li, H.-R., Zhang, W., Hua, Z.-D., Lin, X.-H., Lin, M.-Q., Huang, W.-S., Huang, L.-P., Yu, X.-L., Xu, N.-L., Lin, M., Xie, B.-S., Shen, X.-N., Xie, J.-F., Wang, Y., Huang, M., Wu, Y.-A., Hu, X.-L. Tags: Immunology, Microbiology, Virology Source Type: research