Light therapy modulates serotonin levels and blood flow in women with headache. A preliminary study
In this study, we looked at the possible effects of low-level laser therapy (LLLT) on blood flow velocity, and serotonin (5-HT) and cholinesterase levels in patients with chronic headache associated with temporomandibular disorders (TMD). LLLT has been clinically applied over the past years with positive results in analgesia and without the report of any side effects. The understanding of biological mechanisms of action may improve clinical results and facilitate its indication. Ten patients presenting headache associated with TMD completed the study. An 830-nm infrared diode laser with power of 100 mW, exposure time of 34...
Source: Experimental Biology and Medicine - January 11, 2016 Category: Research Authors: Tomaz de Magalhaes, M., Nunez, S. C., Kato, I. T., Ribeiro, M. S. Tags: Biomedical Engineering Source Type: research
In vivo effects of human adipose-derived stem cells reseeding on acellular bovine pericardium in nude mice
This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus, aid in the reconstruction. To investigate the reseeding of hASCs on ABP grafts by using non-invasive bioluminescence imaging (BLI), and to identify the effective hASCs–scaffold combinations that enabled regeneration. Thirty female athymic nude mice were randomly divided into three groups: In the VIVO group, ABPs were implanted in the subcutaneous pockets and enhanced green fluorescent protein luciferase (eGFP·Luc)-hASCs (1 x ...
Source: Experimental Biology and Medicine - January 11, 2016 Category: Research Authors: Wu, Q., Dai, M., Xu, P., Hou, M., Teng, Y., Feng, J. Tags: Bioimaging Source Type: research
Up-regulation of miR-138 inhibits hypoxia-induced cardiomyocyte apoptosis via down-regulating lipocalin-2 expression
In conclusion, our study demonstrated that up-regulation of miR-138 inhibits the hypoxia-induced cardiomyocyte apoptosis via down-regulating the pro-apoptotic gene expression of Lcn2. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - January 11, 2016 Category: Research Authors: Xiong, H., Luo, T., He, W., Xi, D., Lu, H., Li, M., Liu, J., Guo, Z. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Dietary 2-oxoglutarate mitigates gastrectomy-evoked structural changes in cartilage of female rats
Gastrectomy (Gx) leads to osteopenia/osteoporosis in humans and animals. However, little is known about the influence of Gx on the cartilage in this regard. Recent studies have demonstrated a protective effect of 2-oxoglutaric acid (2-Ox) on bone and cartilage. Hence, the purpose of this study was to investigate whether 2-Ox can mitigate eventual Gx-induced cartilage impairment. Twenty female Sprague-Dawley rats were subjected to Gx and randomly divided into two groups: Gx + 2-Ox and Gx. Another 20 rats were sham-operated (ShO) and randomly divided into two groups: ShO + 2-Ox and ShO. The daily dose of 2-Ox administered to...
Source: Experimental Biology and Medicine - January 11, 2016 Category: Research Authors: Dobrowolski, P., Tomaszewska, E., Kurlak, P., Pierzynowski, S. G. Tags: Anatomy/Pathology Source Type: research
Epithelial-mesenchymal transition: An emerging target in tissue fibrosis
Epithelial-mesenchymal transition (EMT) is involved in a variety of tissue fibroses. Fibroblasts/myofibroblasts derived from epithelial cells contribute to the excessive accumulation of fibrous connective tissue in damaged tissue, which can lead to permanent scarring or organ malfunction. Therefore, EMT-related fibrosis cannot be neglected. This review highlights the findings that demonstrate the EMT to be a direct contributor to the fibroblast/myofibroblast population in the development of tissue fibrosis and helps to elucidate EMT-related anti-fibrotic strategies, which may enable the development of therapeutic intervent...
Source: Experimental Biology and Medicine - January 11, 2016 Category: Research Authors: Li, M., Luan, F., Zhao, Y., Hao, H., Zhou, Y., Han, W., Fu, X. Tags: Minireview Source Type: research
Pyruvate stabilizes electrocardiographic and hemodynamic function in pigs recovering from cardiac arrest
This study tested the hypothesis that pyruvate-enriched resuscitation restores hemodynamic, metabolic, and electrolyte homeostasis following cardiac arrest. Forty-two Yorkshire swine underwent pacing-induced ventricular fibrillation and, after 6 min pre-intervention arrest, 4 min precordial compressions followed by transthoracic countershocks. After defibrillation and recovery of spontaneous circulation, the pigs were monitored for another 4 h. Sodium pyruvate or NaCl were infused i.v. (0.1 mmol·kg–1·min–1) throughout precordial compressions and the first 60 min recovery. In 8 of the 24 NaCl-infus...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Cherry, B. H., Nguyen, A. Q., Hollrah, R. A., Williams, A. G., Hoxha, B., Olivencia-Yurvati, A. H., Mallet, R. T. Tags: Translational Research Source Type: research
Biliary and duodenal drainage for reducing the radiotoxic risk of antineoplastic 131I-hypericin in rat models
Necrosis targeting radiopharmaceutical 131I-hypericin (131I-Hyp) has been studied for the therapy of solid malignancies. However, serious side effects may be caused by its unwanted radioactivity after being metabolized by the liver and excreted via bile in the digestive tract. Thus the aim of this study was to investigate two kinds of bile draining for reducing them. Thirty-eight normal rats were intravenously injected with 131I-Hyp, 24 of which were subjected to the common bile duct (CBD) drainage for gamma counting of collected bile and tissues during 1–6, 7–12, 13–18, and 19–24 h (n = 6 each grou...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Li, Y., Jiang, C., Jiang, X., Sun, Z., Cona, M. M., Liu, W., Zhang, J., Ni, Y. Tags: Translational Research Source Type: research
Glycogen synthase kinase 3{beta} inhibition enhanced proliferation, migration and functional re-endothelialization of endothelial progenitor cells in hypercholesterolemia microenvironment
Hypercholesterolemia impairs the quantity and function of endothelial progenitor cell. We hypothesized that glycogen synthase kinase 3β activity is involved in regulating biological function of endothelial progenitor cells in hypercholesterolemia microenvironment. For study, endothelial progenitor cells derived from apolipoprotein E-deficient mice fed with high-fat diet were used. Glycogen synthase kinase 3β activity was interfered with glycogen synthase kinase 3β inhibitor lithium chloride or transduced with replication defective adenovirus vector expressing catalytically inactive glycogen synthase kinase 3...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Cui, B., Jin, J., Ding, X., Deng, M., Yu, S., Song, M., Yu, Y., Zhao, X., Chen, J., Huang, L. Tags: Systems Biology Source Type: research
Wnt5a attenuates hypoxia-induced pulmonary arteriolar remodeling and right ventricular hypertrophy in mice
In this study, we investigated the possible functions and mechanisms of Wnt5a in HPH in vivo. Recombinant mouse Wnt5a (rmWnt5a) or phosphate buffered saline (PBS) was administered to male C57/BL6 mice weekly from the first day to the end of the two or four weeks after exposed to hypoxia (10% O2). Hypoxia-induced pulmonary hypertension was associated with a marked increase in β-catenin/cyclin D1 expression in lungs. Right ventricular systolic pressure and right ventricular hypertrophy index were reduced in animals treated with rmWnt5a compared with PBS. Histology showed less pulmonary vascular remodeling and right...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Jin, Y., Wang, W., Chai, S., Liu, J., Yang, T., Wang, J. Tags: Physiology Source Type: research
Administration of microparticles from blood of the lipopolysaccharide-treated rats serves to induce pathologic changes of acute respiratory distress syndrome
This study was conducted to investigate the effect of intratracheal and intravenous administration of microparticles (MPs) on developing acute respiratory distress syndrome (ARDS). The blood MPs from lipopolysaccharide-treated rats were collected and examined by transmission electron microscopy (TEM). Cellular source of the MPs was identified by fluorescent-labeled antibodies after the circulating MPs were delivered to naïve rats. Levels of myeloperoxidase (MPO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-10 productions in bronchoalveolar lavage fluid (BALF) and plasma were determined 24 ...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Li, H., Meng, X., Liang, X., Gao, Y., Cai, S. Tags: Pharmacology/Toxicology Source Type: research
In vitro and in vivo study on the effect of antifungal agents on hematopoietic cells in mice
Liposomal amphotericin B, voriconazole, and caspofungin are currently used for systemic and severe fungal infections. Patients with malignant diseases are treated with granulocyte-colony stimulating factor (G-CSF) for the recovery of granulocytes after chemotherapy or hematopoietic cell (HC) transplantation. Since they have a high incidence of fungal infections, they inevitably receive antifungal drugs for treatment and prophylaxis. Despite their proven less toxicity for various cell types comparatively with amphotericin B and the decrease in the number of leukocytes that has been reported as a possible complication in cli...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Samalidou, M., Bougiouklis, D., Vyzantiadis, T.-A., Meletiadis, J., Monokrousos, N., Siotou, E., Sivropoulou, A., Anagnostopoulos, A., Sotiropoulos, D. Tags: Pharmacology/Toxicology Source Type: research
The liver X receptor agonist TO901317 protects mice against cisplatin-induced kidney injury
Liver X receptors are in the nuclear receptor superfamily and are contained in the regulation of lipid and cholesterol metabolism. Besides, liver X receptors are considered crucial regulators of the inflammatory response and innate immunity. The current study evaluates the in vivo effects that the synthetic liver X receptor agonist TO901317 protects against cisplatin-induced kidney injury in mice. Mice received cisplatin administration through a single intraperitoneal injection (20 mg/kg in saline). And then the mice were treated with the TO901317 by daily gavage (10 mg/kg/day) 12 h postcisplatin administration, and c...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Yang, M., Wang, R., Sun, J., Yu, K., Chen, B., Xu, L., Zhao, B., Wang, H. Tags: Pharmacology/Toxicology Source Type: research
Budesonide inhalation ameliorates endotoxin-induced lung injury in rabbits
This study aims to investigate the effects of nebulized budesonide on endotoxin-induced ARDS in a rabbit model. Twenty-four rabbits were randomized into three groups. Rabbits in the control and budesonide groups were injected with endotoxin. Thereafter, budesonide or saline was instilled, ventilated for four hours, and recovered spontaneous respiratory. Peak pressure, compliance, and PaO2/FiO2 were monitored for 4 h. After seven days, PaO2/FiO2 ratios were measured. Wet-to-dry weight ratios, total protein, neutrophil elastase, white blood cells, and percentage of neutrophils in BALF were evaluated. TNF-α, IL-1β,...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Gao, W., Ju, N. Tags: Pharmacology/Toxicology Source Type: research
The effect of thymoquinone treatment on the combined renal and pulmonary toxicity of cisplatin and diesel exhaust particles
Particulate air pollution (PAP) exposure is associated with increased morbidity and mortality, particularly in patients with renal disease. However, there are only a few studies on the interaction between PAP and renal injury, and none on agents that may ameliorate it. We studied the interaction between cisplatin (CP) nephrotoxicity and a single exposure to diesel exhaust particle (DEP) in rats 24 h before sacrifice, and assessed the effect of co-treatment with the active ingredient in Nigella Sativa seed oil, thymoquinone (TQ) thereon. Rats were injected intraperitoneally with CP (6 mg/kg) and four days later, they were e...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Ali, B. H., Al Za'abi, M., Shalaby, A., Manoj, P., Waly, M. I., Yasin, J., Fahim, M., Nemmar, A. Tags: Pharmacology/Toxicology Source Type: research
The 1,2,3-triazole derivative KP-A021 suppresses osteoclast differentiation and function by inhibiting RANKL-mediated MEK-ERK signaling pathway
The triazole family of compounds has been implicated in modulating various biological processes such as inflammation, tumorigenesis, and infection. To our knowledge, this is the first study to demonstrate the effects of 1,2,3-triazole substituted biarylacrylonitrile compounds, including KP-A021, on the differentiation and function of osteoclasts. KP-A021 and its triazole derivatives, at a concentration that does not cause a cytotoxic response in bone marrow macrophages (BMMs), significantly inhibited osteoclast differentiation induced by receptor activator of nuclear factor-B ligand (RANKL) and macrophage colony-stimulatin...
Source: Experimental Biology and Medicine - December 2, 2015 Category: Research Authors: Ihn, H. J., Lee, D., Lee, T., Shin, H.-I., Bae, Y. C., Kim, S.-H., Park, E. K. Tags: Pharmacology/Toxicology Source Type: research
