Doxorubicin resistance mediated by cytoplasmic macrophage colony-stimulating factor is associated with switch from apoptosis to autophagic cell death in MCF-7 breast cancer cells
This study was to explore the effect of macrophage colony-stimulating factor on doxorubicin resistance in MCF-7 breast cancer cells and the possible mechanism. In the study, the human breast cancer cells, MCF-7, were transfected with macrophage colony-stimulating factor. We document that cytoplasmic macrophage colony-stimulating factor induces doxorubicin resistance and inhibits apoptosis in MCF-7 cells. Further studies demonstrated that cytoplasmic macrophage colony-stimulating factor-mediated apoptosis inhibition was dependent on the activation of PI3K/Akt/Survivin pathway. More importantly, we found that macrophage colo...
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Zhang, M., Zhang, H., Tang, F., Wang, Y., Mo, Z., Lei, X., Tang, S. Tags: Pharmacology & amp;amp; Toxicology Source Type: research
Biochemical and biological characterization of Bothriechis schlegelii snake venoms from Colombia and Costa Rica
In conclusion, geographic distribution influences the composition and activities of Bothriechis schlegelii venoms. Bothrops antivenom cross-reacted with these venoms and was partially effective in neutralizing some toxic activities of BsCo and BsCR. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Prezotto-Neto, J. P., Kimura, L. F., Alves, A. F., Gutierrez, J. M., Otero, R., Suarez, A. M., Santoro, M. L., Barbaro, K. C. Tags: Pharmacology & amp;amp; Toxicology Source Type: research
Entropy-based divergent and convergent modular pattern reveals additive and synergistic anticerebral ischemia mechanisms
Module-based network analysis of diverse pharmacological mechanisms is critical to systematically understand combination therapies and disease outcomes. We first constructed drug-target ischemic networks in baicalin, jasminoidin, ursodeoxycholic acid, and their combinations baicalin and jasminoidin as well as jasminoidin and ursodeoxycholic acid groups and identified modules using the entropy-based clustering algorithm. The modules 11, 7, 4, 8 and 3 were identified as baicalin, jasminoidin, ursodeoxycholic acid, baicalin and jasminoidin and jasminoidin and ursodeoxycholic acid-emerged responsive modules, while 12, 8, 15, 1...
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Yu, Y., Zhang, X., Li, B., Zhang, Y., Liu, J., Li, H., Chen, Y., Wang, P., Kang, R., Wu, H., Wang, Z. Tags: Genomics, Proteomics, Bioinformatics Source Type: research
{gamma}-Carboxyethyl hydroxychroman, a metabolite of {gamma}-tocopherol, preserves nitric oxide bioavailability in endothelial cells challenged with high glucose
We reported that supplementation with -tocopherol (-T) in humans limits impairments in endothelial function otherwise induced by postprandial hyperglycemia. Given the rapid metabolism of -T into -carboxyethyl hydroxychroman (-CEHC), we hypothesized that the vasoprotective activities of -T could be attributed to its metabolite -CEHC. To test this, human aortic endothelial cells (HAECs) treated with 0 (vehicle control) or 3 µM -CEHC for 24 h prior to incubation with normal (5 mM) or high (25 mM) glucose for 48 h. High-glucose increased levels of uncoupled endothelial nitric oxide synthase (eNOS) as evidenced by reduced...
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Li, Y., Bharath, L. P., Qian, Y., Ruan, T., Anandh Babu, P. V., Bruno, R. S., Symons, J. D., Jalili, T. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
Effects of iron supplementation in mice with hypoferremia induced by obesity
In conclusion, systemic iron supplementation in mice with hypoferremia induced by obesity did not worsen inflammatory marker or adipose tissue inflammation or the metabolic status established by obesity. Iron deposition was observed in adipose tissue, mainly in macrophages, suggesting that these cells have mechanisms that promote iron incorporation without increasing the production of inflammatory mediators. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Gotardo, E. M. F., Caria, C. R. e. P., de Oliveira, C. C., Rocha, T., Ribeiro, M. L., Gambero, A. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
Tanshinone IIA ameliorates apoptosis of cardiomyocytes induced by endoplasmic reticulum stress
The fat-soluble diterpenoids tanshinone IIA (TSA) is the major active element of Danshen, which has widespread cardioprotective effect. However, the mechanism of its beneficial effect on cardiomyocytes has not been fully investigated. Here, we aim to demonstrate that TSA ameliorates apoptosis of cardiomyocytes activated by endoplasmic reticulum stress (ERS). Primary cultures of neonatal rat cardiomyocytes are used, in which ERS-mediated apoptosis is induced by tunicamycin (Tm). Apoptosis of cardiomyocytes are detected by Hoechst staining and caspase 3 activity analysis. Protein expression of ERS markers are detected by Wes...
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Feng, J., Li, S., Chen, H. Tags: Cell and Developmental Biology Source Type: research
Induction of matrix metalloproteinase-1 by tumor necrosis factor-{alpha} is mediated by interleukin-6 in cultured fibroblasts of keratoconus
In conclusion, these results indicate that fibroblasts of keratoconus shows increased levels of IL-6 and MMP-1 gene and protein expression and IL-6 mediates the TNF-α-induced MMP-1 expression. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: Du, G., Liu, C., Li, X., Chen, W., He, R., Wang, X., Feng, P., Lan, W. Tags: Cell and Developmental Biology Source Type: research
Featured Article: Nanoenhanced matrix metalloproteinase-responsive delivery vehicles for disease resolution and imaging
The wide array of proteases, including matrix metalloproteinases, produced in response to many pathogenic insults, confers a unique proteolytic signature which is often disease specific and provides a potential therapeutic target for drug delivery. Here we propose the use of collagen-based nanoenhanced matrix metalloproteinase-responsive delivery vehicles that display matrix metalloproteinase-specific degradation in diverse in vitro models of proteolysis. We demonstrate that collagen particles comprised of protease substrates (primarily collagen) can be made of uniform size and loaded efficiently with assorted cargo i...
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: McCarthy, D. A., Nazem, A. A., McNeilan, J., Shakerley, N. L., Clark, R. R., Idelchik, M. D., Yigit, M., Melendez, J. A. Tags: Bionanoscience Source Type: research
The SMAC mimetic BV6 induces cell death and sensitizes different cell lines to TNF-{alpha} and TRAIL-induced apoptosis
In conclusion, therapeutic targeting of IAPs by BV6 might be an effective approach to enhance cancer regression induced by immune system. Our data also open up the future possibility of using BV6 in combination with other antitumor therapies to overcome cancer drug resistance. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - November 7, 2016 Category: Research Authors: El-Mesery, M., Shaker, M. E., Elgaml, A. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
The efficacy of novel anatomical sites for the assessment of muscle oxygenation during central hypovolemia
Muscle tissue oxygenation (SmO2) can track central blood volume loss associated with hemorrhage. Traditional peripheral measurement sites (e.g., forearm) may not be practical due to excessive movement or injury (e.g., amputation). The aim of this study was to evaluate the efficacy of three novel anatomical sites for the assessment of SmO2 under progressive central hypovolemia. 10 male volunteers were exposed to stepwise prone lower body negative pressure to decrease central blood volume, while SmO2 was assessed at four sites—the traditional site of the flexor carpi ulnaris (ARM), and three novel sites not previously ...
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Sprick, J. D., Soller, B. R., Rickards, C. A. Tags: Physiology Source Type: research
Cardiac rehabilitation improves the blood plasma properties of cardiac patients
Cardiac rehabilitation (CR) improves exercise tolerance and general function. However, its effects on blood plasma in cardiac patients remain uncertain. Our aim was to examine the effect of comprehensive CR on the oxidative stress parameters and antioxidant plasma status in patients with coronary artery disease (CAD) after cardiac interventions. Exercise-based rehabilitation was established as ergometer training, adjusted for individual patients’ physical efficiency. Training was repeated three times a week for two months. The standard biochemical (total cholesterol, HDL, LDL, triglycerides and erythrocyte sedimentat...
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Gwozdzinski, K., Pieniazek, A., Czepas, J., Brzeszczynska, J., Jegier, A., Pawlicki, L. Tags: Physiology Source Type: research
Effect of fermented porcine placenta on physical fatigue in mice
In conclusion, these findings suggest the potential of FPP as a novel functional food for the regulation of fatigue. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Kim, H.-Y., Han, N.-R., Kim, N.-R., Lee, M., Kim, J., Kim, C.-J., Jeong, H.-J., Kim, H.-M. Tags: Pharmacology & amp;amp; Toxicology Source Type: research
Rule of accumulation of enrofloxacin in Acipenser baerii and drug-induced damage to the tissues
Enrofloxacin (ENX) has been widely used in the prevention and control of bacterial diseases in sturgeon aquaculture due to its characteristics of wide antibacterial spectrum, strong antibacterial activity, less toxicity and fewer side effects, rapid action, extensive in vivo distribution, and little cross-resistance with other antibiotics. However, the spinal abnormality was found in Acipenser baerii soon after ENX administration, which resulted an "S"-shaped curvature of the spine and retarded fish growth. It was still not clear whether ENX could cause spinal abnormality in sturgeons by now. The aim of this work was ...
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Wang, D., Li, S., Lu, T. Tags: Pharmacology & amp;amp; Toxicology Source Type: research
Glycoprotein non-metastaticmelanoma protein B promotes glioma motility and angiogenesis through the Wnt/{beta}-catenin signaling pathway
In conclusion, we demonstrate that glycoprotein non-metastatic melanoma protein B might be an inducer for glioma and could enhance matrix metalloproteinase activity through Wnt/β-catenin pathway to contribute to glioma tumorigenesis. This may represent a new understanding for malignant glioma. (Source: Experimental Biology and Medicine)
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Bao, G., Wang, N., Li, R., Xu, G., Liu, P., He, B. Tags: Neuroscience Source Type: research
Plasma lipid levels predict dysglycemia in a biracial cohort of nondiabetic subjects: Potential mechanisms
Dyslipidemia and dysglycemia are etiologically associated, but the direction, chronology, and mechanisms of the association are not fully understood. We, therefore, analyzed data from 335 healthy adults (184 black, 151 white) enrolled in the Pathobiology of Prediabetes in A Biracial Cohort study. Subjects underwent oral glucose tolerance test (OGTT) and were enrolled if they had normal fasting and 2-h plasma glucose levels. Assessments during year 1 included anthropometry, fasting lipid profile, insulin sensitivity, and insulin secretion. Thereafter, OGTT was assessed annually for 5.5 years. The primary outcome was occurre...
Source: Experimental Biology and Medicine - October 3, 2016 Category: Research Authors: Owei, I., Umekwe, N., Wan, J., Dagogo-Jack, S. Tags: Endocrinology & amp;amp; Nutrition Source Type: research
