The Prevalence of CCR5 ‐Δ32 Mutation in a Cohort of Saudi Stem Cell Donors
In this study, our aim was to investigate the frequency of CCR5‐Δ32 mutation in a cohort of stem cell donors from cord blood bank and stem cell donor registry. A total of 3025 samples were collected from healthy stem cell donors (2625) and from cord blood units (400). DNA was extracted and the CCR5 gene was amplified by polymerase chain reaction (PCR) in a light cycler system using SYBR Green dye. The mutated gene was further confirmed by direct gene sequencing. We found 38 heterozygous for CCR5‐Δ32 and one homozygous CCR5 mutation (Δ32/Δ32) out of the 3025 tested individuals. We conclude that the protective CCR5...
Source: Tissue Antigens - July 21, 2017 Category: Allergy & Immunology Authors: M. Alarifi, F. Al ‐Amro, A. Alalwan, A. Al‐Turki, H. Fakhoury, N. Atallah, M. Al Muallimi, M. Al‐Balwi, M. ALzahrani, A. Alaskar, A. Hajeer, D. Jawdat Tags: BRIEF COMMUNICATION Source Type: research
Detection of a novel allele, HLA ‐A*24:198, by sequence‐based typing in a Chinese individual
HLA‐A*24:198 has one nucleotide change from HLA‐A*24:02:01 where Aspartic Acid (29) is changed to Asparagine. (Source: Tissue Antigens)
Source: Tissue Antigens - July 21, 2017 Category: Allergy & Immunology Authors: Kai Wang, Xiao ‐Feng Li, Xu Zhang, Feng‐Qiu Lin, Jian‐Ping Li Tags: NEW ALLELE ALERTS Source Type: research
Human leukocyte antigen distribution and genomic ancestry in Brazilian patients with sickle cell disease
Hematopoietic stem‐cell transplantation (HSCT) is currently the only established curative treatment for sickle cell disease (SCD), but is limited by donor availability. Ethnicity is thought to have an impact on the complications experienced by patients that undergo HSCT and on the likelihood of identifying an HLA matched donor. In the present study, we investigated the genomic ancestry and the distribution of HLA allele groups in Brazilian patients with SCD, compared these HLA profiles to worldwide populations and evaluate the availability of HLA‐matched donors. A broad intercontinental admixture of patients with SCD w...
Source: Tissue Antigens - July 21, 2017 Category: Allergy & Immunology Authors: M. C. F. da Silva ‐Malta, P. S. Rodrigues, L. W. Zuccherato, F. C. B. de Souza, E. M. F. L. Domingues, V. R. Souza, E. Tarazona‐Santos, M. L. Martins Tags: ORIGINAL ARTICLE Source Type: research
HLA ‐B*40:01:45, a novel variant of HLA‐B*40:01, discovered in a Taiwanese hematopoitic stem cell donor
Abstract
Two nucleotide replacements within HLA‐B*40:01:01 result in the novel allele, HLA‐B*40:01:45. (Source: Tissue Antigens)
Source: Tissue Antigens - July 20, 2017 Category: Allergy & Immunology Authors: K. L. Yang, P. Y. Lin Tags: NEW ALLELE ALERTS Source Type: research
HFE gene polymorphism defined by sequence based typing of the Brazilian population and a standardized nomenclature for HFE allele sequences
Abstract
The HFE molecule controls iron uptake from gut, and defects in the molecule have been associated with iron overload, particularly in hereditary hemochromatosis. The HFE gene including both coding and boundary intronic regions were sequenced in 304 Brazilian individuals, encompassing healthy individuals and patients exhibiting hereditary or acquired iron overload. Six sites of variation were detected: i) H63D C > G in exon 2, ii) IVS2 (+4) T > C in intron 2, iii) a C > G transversion in intron 3, iv) C282Y G > A in exon 4, v) IVS4 (−44) T > C in intron 4, and vi) a new Guanine d...
Source: Tissue Antigens - July 20, 2017 Category: Allergy & Immunology Authors: W.N Campos, J.D Massaro, A.L.C Martinelli, J.A Halliwell, S.G.E Marsh, C.T Mendes ‐Junior, E.A Donadi Tags: BRIEF COMMUNICATION Source Type: research
Identification of a novel allele, HLA ‐C*02:02:33, by full‐length genomic sequencing
Summary
The HLA‐C*02:02:33 allele differs by a single nucleotide at codon 215 compared with HLA‐C*02:02:02:01. (Source: Tissue Antigens)
Source: Tissue Antigens - July 20, 2017 Category: Allergy & Immunology Authors: Y. Park, H. Kim, J. Im, C. E. Yoon, H. S. Kim Tags: NEW ALLELE ALERTS Source Type: research
Discovery of a novel HLA ‐A*02 variant, HLA‐A*02:684, in a Taiwanese individual
Abstract
One nucleotide replacement at residue 728 of HLA‐A*02:03:01 results in a new allele, HLA‐A*02:684. (Source: Tissue Antigens)
Source: Tissue Antigens - July 20, 2017 Category: Allergy & Immunology Authors: K. L. Yang, P. Y. Lin Tags: NEW ALLELE ALERTS Source Type: research
Full ‐length sequencing of the HLA region identified a novel allele, HLA‐B*52:70
One nucleotide substitution at position 276 of HLA‐B*52:01:01 results in a novel allele, HLA‐B*52:70. (Source: Tissue Antigens)
Source: Tissue Antigens - July 20, 2017 Category: Allergy & Immunology Authors: X. D. Mo, J. Wang, T. Zhang Tags: NEW ALLELE ALERTS Source Type: research
Identification of the HLA ‐DQB1*06:123 allele in an unrelated stem cell donor from the Saudi Registry
HLA‐DQB1*06:123 differs from HLA‐DQB1*06:29 by six nucleotide substitutions resulting in five amino acid changes. (Source: Tissue Antigens)
Source: Tissue Antigens - July 18, 2017 Category: Allergy & Immunology Authors: H. A. Fakhoury, M. Alzahrani, A. S. Alaskar, A. H. Hajeer Tags: NEW ALLELE ALERTS Source Type: research
A novel allele, HLA ‐C*14:02:01:03, identified by full‐length genomic sequencing
The HLA‐C*14:02:01:03 differs by a single nucleotide at intron 5 (2157 C>T) compared with C*14:02:01:01. (Source: Tissue Antigens)
Source: Tissue Antigens - July 18, 2017 Category: Allergy & Immunology Authors: Y. Park, B. G. Park, J. Im, C. E. Yoon, H. S. Kim Tags: NEW ALLELE ALERTS Source Type: research
The full ‐length sequence of HLA‐ B*59:01:01:01 confirmed by cloning and sequencing
Summary
The full‐length sequence of HLA‐ B*59:01:01:01.
The HLA genes are understood to be the most polymorphic in the human genome. According to the IPD‐IMGT/HLA Database (release 3.27.0 2017‐01), a total of 4,647 HLA‐B alleles have been identified to date (1). However, members of the B*59 gene family are scarce and have only 10 alleles. (Source: Tissue Antigens)
Source: Tissue Antigens - July 18, 2017 Category: Allergy & Immunology Authors: Z. Li, H. ‐Y. Zou, D. Zhou Tags: NEW ALLELE ALERTS Source Type: research
Identification of a novel HLA ‐B allele, HLA‐B*40:238, in a Taiwanese individual
Summary
The HLA‐B*40:238 allele has one non‐synonymous transversion from HLA‐B*40:01:01 at nucleotide position 484.
The HLA‐B*40:238 allele was found in an association study between HLA and antithyroid drug‐induced agranulocytosis 1. The allele, initially genotyped in a Graves' disease patient showed one mismatched nucleotide within the combined sequence pattern of B*40:01:01 and B*13:01:01 using SeCore HLA Sequence‐based typing kit (Life Technologies Corporation, WI, USA). To determine which allele the mismatch belonged to, polymerase chain reaction (PCR) was carried out to amplify exon 2 to exon 4 of the HLA...
Source: Tissue Antigens - July 17, 2017 Category: Allergy & Immunology Authors: Hui ‐Lin Lee, Sheng‐Kai Lai, Pei‐Lung Chen, Chen‐Chung Chu Tags: NEW ALLELE ALERTS Source Type: research
A novel HLA class II allele, HLA ‐DQB1*03:73
The HLA‐DQB1*03:73 allele differs from DQB1*03:01:01:01 by 1 nucleotide substitution at position 209. (Source: Tissue Antigens)
Source: Tissue Antigens - July 14, 2017 Category: Allergy & Immunology Authors: J. W. Zheng, J. H. Xie, Y. Sun, Y. Q. Jiang, D. Xiang Tags: NEW ALLELE ALERTS Source Type: research
Identification of a novel allele, HLA ‐C*01:135, by full‐length genomic sequencing
The HLA‐C*01:135 allele differs by a single nucleotide at codon 265 compared with HLA‐C*01:02:01:01. (Source: Tissue Antigens)
Source: Tissue Antigens - July 14, 2017 Category: Allergy & Immunology Authors: Y. Park, J. Im, C. E. Yoon, H. S. Kim Tags: NEW ALLELE ALERTS Source Type: research
Issue Information
(Source: Tissue Antigens)
Source: Tissue Antigens - July 13, 2017 Category: Allergy & Immunology Tags: ISSUE INFORMATION Source Type: research
