NGS typings reveal very high frequencies of particular HLA class II alleles and haplotypes
Abstract
A total of 72 unrelated Mandenka individuals from Eastern Senegal, Niokholo region, were typed using Next Generation Sequencing (library preparation with the Holotype HLA X2 and MIA FORA NGS HLA Typing kits, sequencing with Illumina MiSeq, and bio‐informatic processing with HLA Twin v1.1.1 (Omixon) and MIA FORA NGS software) and yielded reliable genotypes for 8 HLA loci, namely A, B, C, DRB1, DQA1, DQB1, DPA1 and DPB1. (Source: Tissue Antigens)
Source: Tissue Antigens - December 27, 2017 Category: Allergy & Immunology Authors: T. Goeury, L. E. Creary, M. A. Fernandez ‐Vina, J.‐M. Tiercy, J. M. Nunes, A. Sanchez‐Mazas Tags: Population Report Source Type: research
Characterization of two New HLA ‐B Alleles, HLA‐B*07:299 and HLA‐B*35:350
ABSTRACT
Two novel HLA‐B alleles, B*07:299 and B*35:350, were characterized by genomic full‐length sequencing. (Source: Tissue Antigens)
Source: Tissue Antigens - December 26, 2017 Category: Allergy & Immunology Authors: A. Balas, R. Alenda, R. L ópez‐Hernández, F. Sánchez‐García, J. L. Vicario Tags: NEW ALLELE ALERTS Source Type: research
A novel HLA ‐B allele: HLA‐B*40:241
Abstract
The HLA‐B*40:241 allele differs from B*40:01:01 by one nucleotide substitution at position 691 . (Source: Tissue Antigens)
Source: Tissue Antigens - December 26, 2017 Category: Allergy & Immunology Authors: J. W. Zheng, W. Shen, Y. Sun, J. H. Xie, D. Xiang Tags: NEW ALLELE ALERTS Source Type: research
The killer cell lectin ‐like receptor B1 (KLRB1) 503T > C polymorphism (rs1135816) and acute rejection after liver transplantation
The killer cell lectin‐like receptor B1 (KLRB1) gene encodes for CD161 expressed by different subsets of leukocytes involved in the development of acute liver transplant rejection. The single nucleotide polymorphism (SNP) 503T>C (rs1135816) in the KLRB1 gene represents a missense mutation modifying functional properties of CD161. The aim of our study is to determine whether the SNP 503T>C is associated with acute liver transplant rejection. We genotyped the SNP for 163 liver recipients without acute rejection, 125 recipients with a single acute rejection, and 53 recipients with multiple acute rejections. The genoty...
Source: Tissue Antigens - December 25, 2017 Category: Allergy & Immunology Authors: H. Thude, S. Rother, M. Sterneck, J. Klempnauer, B. Nashan, R. Schwinzer, M. Koch Tags: BRIEF COMMUNICATION Source Type: research
HLAs: Key regulators of T ‐cell‐mediated drug hypersensitivity
Adverse drug reactions (ADR) can be broadly categorised as either on‐target or off‐target. On‐target ADRs arise as a direct consequence of the pharmacological properties of the drug and are therefore predictable and dose‐dependent. On‐target ADRs comprise the majority (>80%) of ADRs, relate to the drug's interaction with its known pharmacological target and are a result of a complex interplay of genetic and ecologic factors. In contrast, off‐target ADRs, including immune‐mediated ADRs (IM‐ADRs), are due to unintended pharmacological interactions such as inadvertent ligation of host cell receptors or non...
Source: Tissue Antigens - December 25, 2017 Category: Allergy & Immunology Authors: A. J. Redwood, R. K. Pavlos, K. D. White, E. J. Phillips Tags: REVIEW ARTICLE Source Type: research
Issue Information
(Source: Tissue Antigens)
Source: Tissue Antigens - December 25, 2017 Category: Allergy & Immunology Tags: ISSUE INFORMATION Source Type: research
Single molecule real ‐time DNA sequencing of HLA genes at ultra‐high resolution from 126 International HLA and Immunogenetics Workshop cell lines
The hyperpolymorphic HLA genes play important roles in disease and transplantation and act as genetic markers of migration and evolution. A panel of 107 B‐lymphoblastoid cell lines (B‐LCLs) was established in 1987 at the 10th International Histocompatibility Workshop as a resource for the immunogenetics community. These B‐LCLs are well characterised and represent diverse ethnicities and HLA haplotypes. Here we have applied Pacific Biosciences’ Single Molecule Real‐Time (SMRT) DNA sequencing to HLA type 126 B‐LCL, including the 107 International HLA and Immunogenetics Workshop (IHIW) cells, to ultra‐high resol...
Source: Tissue Antigens - December 20, 2017 Category: Allergy & Immunology Authors: T. R. Turner, J. D. Hayhurst, D. R. Hayward, W. P. Bultitude, D. J. Barker, J. Robinson, J. A. Madrigal, N. P. Mayor, S. G. E. Marsh Tags: ORIGINAL ARTICLE Source Type: research
Identification of the novel HLA ‐DQB1*03:181 allele in a Chinese leukemia patient
Summary
HLA‐DQB1*03:181 has one nucleotide change from HLA‐DQB1*03:05:01 at position 470 C>G. (Source: Tissue Antigens)
Source: Tissue Antigens - December 8, 2017 Category: Allergy & Immunology Authors: Z. ‐D. Hang, L.‐N. Dong, S.‐X. Zhao, B. Dai, F.‐M. Zhu Tags: NEW ALLELE ALERTS Source Type: research
HLA ‐DQB1*02:102, a novel allele identified by next‐generation sequencing in a Spanish individual
HLA‐DQB1*02:102 differs from DQB1*02:01:01 by a single‐nucleotide substitution resulting in an amino acid change. (Source: Tissue Antigens)
Source: Tissue Antigens - December 5, 2017 Category: Allergy & Immunology Authors: E. Enrich, L. Mongay, J. L. Caro ‐Oleas, M. J. Herrero‐Mata, F. Rudilla Tags: NEW ALLELE ALERTS Source Type: research
The novel allele, HLA ‐A*31:108, identified by sequence‐based typing in a Chinese individual
The HLA‐A*31:108 allele was found. (Source: Tissue Antigens)
Source: Tissue Antigens - December 5, 2017 Category: Allergy & Immunology Authors: H. Ding, Y. Huang, Y. Chen, X. Ye Tags: NEW ALLELE ALERTS Source Type: research
Identification of the novel HLA ‐B*13:98 allele in a Chinese individual
Summary
HLA‐B*13:98 differs from HLA‐B*13:02:01 by a single nucleotide substitution at position 193 A > G. (Source: Tissue Antigens)
Source: Tissue Antigens - December 1, 2017 Category: Allergy & Immunology Authors: N ‐Y. Chen, S‐X. Zhao, L‐N Dong, J. Xu, F‐M. Zhu Tags: NEW ALLELE ALERTS Source Type: research
Comparison of two peripheral mononuclear cell isolation protocols for flow cytometry crossmatching
Abstract
In clinical organ transplantation, flow cytometry crossmatching can be performed on total blood with a hemolysis step or after a preliminary mononuclear cell separation step using a standard Ficoll‐Hypaque protocol. Here, we compared the Ficoll‐Hypaque step with a faster technique for isolating mononuclear cells (the SepMateTM tube), using the same samples (collected and stored at room temperature for 0, 24, 48 or 72 hours). We found that the SepMateTM separation protocol is easily applied to flow cytometry crossmatching (with or without pronase treatment), provided that the samples have been stored at room te...
Source: Tissue Antigens - December 1, 2017 Category: Allergy & Immunology Authors: M. ‐J. Apithy, J. Desoutter, N. Guillaume Tags: BRIEF COMMUNICATION Source Type: research
Pitfalls in Graft Survival Analysis
ABSTRACT
The reliability of a scientific work depends on the accuracy of the analysis. Scientific publications in the field of kidney transplantation still contain methodical errors that may lead to wrong conclusions and result in severe consequences for the patients. Using the data from the Collaborative Transplant Study, we are presenting in this article six examples of the erroneous usage of statistical methods and demonstrate how these mistakes can be avoided. (Source: Tissue Antigens)
Source: Tissue Antigens - December 1, 2017 Category: Allergy & Immunology Authors: C. Unterrainer, B. D öhler, C. Süsal Tags: REVIEW ARTICLE Source Type: research
Genetic history of the African Sahelian populations
Abstract
From a biogeographic perspective Africa is subdivided into distinct horizontal belts. Human populations living along the Sahel/Savannah belt south of the Sahara Desert have often been overshadowed by extensive studies focusing on other African populations such as hunter‐gatherers or Bantu in particular. However, the Sahel together with the savannah bordering it in the south, is a challenging region where people had and still have to cope with harsh climatic conditions and show resilient behaviours. Besides exponentially growing urban populations, several local groups leading various lifestyles and speaking langu...
Source: Tissue Antigens - December 1, 2017 Category: Allergy & Immunology Authors: V. Černý, I. Kulichová, E. S. Poloni, J. M. Nunes, L. Pereira, A. Mayor, A. Sanchez‐Mazas Tags: REVIEW ARTICLE Source Type: research
Hla ‐a*30:125 shows a new antigen binding domain created from hla‐a*30:01 and hla‐a*30:02
ABSTRACT
A new HLA‐A allele, A*30:125, was characterized in a Spanish individual. (Source: Tissue Antigens)
Source: Tissue Antigens - November 30, 2017 Category: Allergy & Immunology Authors: A. Balas, R. Alenda, M. A. Moreno ‐Hidalgo, F. García‐Sánchez, J. L. Vicario Tags: NEW ALLELE ALERTS Source Type: research
