Dual redundant sequencing strategy: Full ‐length gene characterisation of 1056 novel and confirmatory HLA alleles
The high‐throughput department of DKMS Life Science Lab encounters novel human leukocyte antigen (HLA) alleles on a daily basis. To characterise these alleles, we have developed a system to sequence the whole gene from 5′‐ to 3′‐UTR for the HLA loci A, B, C, DQB1 and DPB1 for submission to the European Molecular Biology Laboratory – European Nucleotide Archive (EMBL‐ENA) and the IPD‐IMGT/HLA Database. Our workflow is based on a dual redundant sequencing strategy. Using shotgun sequencing on an Illumina MiSeq instrument and single molecule real‐time (SMRT) sequencing on a PacBio RS II instrument, we are ab...
Source: Tissue Antigens - May 26, 2017 Category: Allergy & Immunology Authors: V. Albrecht, C. Zweiniger, V. Surendranath, K. Lang, G. Sch öfl, A. Dahl, S. Winkler, V. Lange, I. Böhme, A. H. Schmidt Tags: ORIGINAL ARTICLE Source Type: research
The DRB1*15:11 allele discovered in a Taiwanese unrelated hematopoietic stem cell donor
One nucleotide substitution at residue 227 of HLA‐DRB1*15:02:01:01 results in a new allele, HLA‐DRB1*15:11. (Source: Tissue Antigens)
Source: Tissue Antigens - May 25, 2017 Category: Allergy & Immunology Authors: K. L. Yang, P. Y. Lin Tags: NEW ALLELE ALERTS Source Type: research
Identification of a novel HLA ‐B*27 variant, B*27:112, by sequence‐based typing in a Taiwanese donor
One nucleotide substitution at residue 74 of HLA‐B*27:04:01 results in a novel allele, HLA‐B*27:112. (Source: Tissue Antigens)
Source: Tissue Antigens - May 24, 2017 Category: Allergy & Immunology Authors: K. L. Yang, P. Y. Lin Tags: NEW ALLELE ALERTS Source Type: research
Identification of a novel HLA allele, HLA ‐B*41:50, in a French individual
The novel HLA‐B*41:50 allele differs from HLA‐B*41:01:01 by a single nucleotide substitution at codon 116. (Source: Tissue Antigens)
Source: Tissue Antigens - May 16, 2017 Category: Allergy & Immunology Authors: B. Proust, D. Masson, B. P. Proust, M. Baudron, F. Dehaut Tags: NEW ALLELE ALERTS Source Type: research
TypeLoader: A fast and efficient automated workflow for the annotation and submission of novel full ‐length HLA alleles
Recent years have seen a rapid increase in the discovery of novel allelic variants of the human leukocyte antigen (HLA) genes. Commonly, only the exons encoding the peptide binding domains of novel HLA alleles are submitted. As a result, the IPD‐IMGT/HLA Database lacks sequence information outside those regions for the majority of known alleles. This has implications for the application of the new sequencing technologies, which deliver sequence data often covering the complete gene. As these technologies simplify the characterization of the complete gene regions, it is desirable for novel alleles to be submitted as full...
Source: Tissue Antigens - May 15, 2017 Category: Allergy & Immunology Authors: V. Surendranath, V. Albrecht, J. D. Hayhurst, B. Sch öne, J. Robinson, S. G. E. Marsh, A. H. Schmidt, V. Lange Tags: ORIGINAL ARTICLE Source Type: research
Author Index
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: AUTHOR INDEX Source Type: research
Poster Presentations
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: POSTER PRESENTATIONS Source Type: research
Mini Oral Presentations
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: MINI ORAL PRESENTATIONS Source Type: research
Oral Presentations
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: ORAL PRESENTATIONS Source Type: research
Forewords
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: FOREWORD Source Type: research
Abstract Book
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: ABSTRACT BOOK Source Type: research
Issue Information
(Source: Tissue Antigens)
Source: Tissue Antigens - May 9, 2017 Category: Allergy & Immunology Tags: ISSUE INFORMATION Source Type: research
Assessing the impact of FoxP3 and Vav1 gene polymorphisms on kidney allograft survival
FoxP3 and Vav1 are known to be involved in the development of regulatory T cells. Two polymorphic sites in the FoxP3 promoter (rs3761548 and a (GT)
n
‐dinucleotide repeat) and 2 single nucleotide polymorphisms in intron 1 of the Vav1 gene (rs2546133 and rs2617822) have been shown to correlate with gene expression levels. We investigated a potential impact of FoxP3 and Vav1 genetic variants on kidney allograft failure using samples and data of the Collaborative Transplant Study. A cohort of 384 kidney transplant patients was tested. We found no significant association of FoxP3 promoter rs3761548 or (GT)
n
repeat length w...
Source: Tissue Antigens - May 3, 2017 Category: Allergy & Immunology Authors: M. Adamek, B. D öhler, K. K. Hasan, G. Fiedler, S. Scherer, G. Opelz, T. H. Tran Tags: BRIEF COMMUNICATION Source Type: research
Identification of the novel
HLA ‐DQB1*02:85 and
HLA‐DRB1*01:01:30 alleles in Russian individuals
Two novel HLA class II alleles,
HLA‐DQB1*02:85 and
HLA‐DRB1*01:01:30, were detected in Russian individuals. (Source: Tissue Antigens)
Source: Tissue Antigens - May 1, 2017 Category: Allergy & Immunology Authors: E. Kuzmich, S. Tyapushkina, A. Alyanskiy, B. Afanasyev Tags: NEW ALLELE ALERTS Source Type: research
Identification of a novel HLA ‐B allele,
HLA‐B*08:177, in a Hungarian patient and her HLA identical sibling
The newly detected HLA‐B*08:177 differs from HLA‐B*08:01:01:01 by 1 single nucleotide substitution at position 365 of exon 3. (Source: Tissue Antigens)
Source: Tissue Antigens - May 1, 2017 Category: Allergy & Immunology Authors: D. Inotai, Z. Ill és, E. Szabó, Á. Varga, A. Szilvási Tags: NEW ALLELE ALERTS Source Type: research
