The Prevalence of CCR5 ‐Δ32 Mutation in a Cohort of Saudi Stem Cell Donors
In this study, our aim was to investigate the frequency of CCR5‐Δ32 mutation in a cohort of stem cell donors from cord blood bank and stem cell donor registry. A total of 3025 samples were collected from healthy stem cell donors (2625) and from cord blood units (400). DNA was extracted and the CCR5 gene was amplified by polymerase chain reaction (PCR) in a light cycler system using SYBR Green dye. The mutated gene was further confirmed by direct gene sequencing. We found 38 heterozygous for CCR5‐Δ32 and one homozygous CCR5 mutation (Δ32/Δ32) out of the 3025 tested individuals. We conclude that the protective CCR5‐Δ32 allele appears to be rarely present in Saudi Arabia.
Source: Tissue Antigens - Category: Allergy & Immunology Authors: M. Alarifi, F. Al ‐Amro, A. Alalwan, A. Al‐Turki, H. Fakhoury, N. Atallah, M. Al Muallimi, M. Al‐Balwi, M. ALzahrani, A. Alaskar, A. Hajeer, D. Jawdat Tags: BRIEF COMMUNICATION Source Type: research
More News: Allergy & Immunology | Genetics | Middle East Health | Saudi Arabia Health | Stem Cell Therapy | Stem Cells | Study | Transplants