Journal of Equine Veterinary Science This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Serum Testosterone Levels and Seminal Plasma Proteins in Castrated Stallions
Testosterone is the major circulating androgen in males; essential for the development and maintenance of secondary sexual characteristics as well as initiation and maintenance of spermatogenesis [1]. Seminal plasma is a complex fluid composed of secretions produced by testes, epididymides and accessory sex glands (AG) and contains several components that play important roles in sperm maturation and fertilization [2]. It is know that the AG epithelium is androgen-dependent; the decrease of the testosterone levels induces a deficient function of these glands [3] and in rams, causes changes in seminal plasma proteome [4]. (S...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: P.N. Guasti, P.M. Papa, R.A. Schmith, L.S. Camargo, L.R.P. Andrade, L.F.M.C. Silva, C.P. Freitas-DellAqua, F.F. Souza, F.O. Papa Source Type: research

Effect of a Photostimulating Treatment, in Winter, on Steroids Profile and Sexual Behavior of Light Stallions
Horses ’ reproductive activity is seasonal with a period of sexual rest during short days. In the mare, this period of complete cessation of reproduction can be shortened by a photostimulation starting near the shortest day of the years with 14h30 of light per day with a minimum of 10 lux [1]. In the sta llion, this period of sexual rest is mainly characterized by a decrease in sexual motivation [2]. However, the effect of photostimulating treatment on reproductive function of stallions, particularly on sexual behavior or testosterone level, is not clearly established: some authors found clear effec ts [3] while some oth...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: D. Guillaume, L. Grosboillot, D. Chesneau, F. De Geoffroy, S. Prevost, M. Keller, G. Dervilly-Pinel Source Type: research

Comparison of Two Methods for Stallion Sperm Vitrification
Sperm vitrification is based on high-speed cooling by direct plunging of small volumes of sperm into liquid nitrogen without any permeable cryoprotectant, adding instead carbohydrates and proteins. This technique could be performed with direct contact of the sperm with liquid nitrogen (non-aseptic technique) or using straws that allows the isolation of the sperm from liquid nitrogen, therefore reducing the potential risk of microbial contamination (Isachenko et al. Andrology. 2017; 5; 1055-1063). (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: C. Consuegra, F. Crespo, M. Diaz-Jimenez, I. Ortiz, B. Pereira, M. Bottrel, J. Dorado, M. Hidalgo Source Type: research

Toxicity of Latex Rubber in Artificial Vaginas on Equine Sperm
Three experiments were conducted. Experiment 1 evaluated the effects of 24-h exposure of extender (INRA96, IMV Technologies, L ’Aigle, France) to rubber at four different temperatures (4°, 22°, 38°, or 45°C) on semen quality. Artificial vagina (AV) rubber from 3 sources (Reproduction Resources [RR], Animal Reproduction Systems [ARS], and NASCO) was subjected to several cleaning methods (DI water only [R], washed with detergent and rinsed with DI water [D], or soaked in alcohol and rinsed with DI water [A]). (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: W.W. Hohertz, J.M. Duarte, K.A. LaCaze, S.R. Teague, D.D. Varner, C.C. Love Source Type: research

Fixable Stain for Flow Cytometric Evaluation of Stallion Sperm Viability
Sperm membrane integrity is an important component of stallion sperm quality and fertility potential [1] that is typically assessed at the region of the sperm head and considered a measurement of viable or live sperm [2]. Flow cytometric assessment of viability involves the collection and transport of live sperm to a laboratory for evaluation, with assays run on unfixed sperm. A double-stain assay uses SYBR-14, a penetrating nucleic acid green stain, and propidium iodide (PI), a red stain that labels the nucleic acid of membrane-compromised sperm [3]. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: J.M. Trentin, R.A. Gonzalez-Castro, M.I.B. Rubin, J.K. Graham, E.M. Carnevale Source Type: research

Evaluation of Fertilization Capacity of Cryopreserved Stallion Sperm, Directly After Thawing and After Cooled Storage
In the equine breeding the use of cryopreserved semen is the method of choice for stallions involved in sport. Cryopreserved semen can be stored and used whenever needed. However, this requires specialized equipment for transportation, storage and further handling of semen; for which not all practitioners are equipped. The aim of our experiments was to investigate if cryopreserved sperm maintains its fertilizing capacity after thawing and cooled transport. Therefore, we determined: (1) viability of cryopreserved sperm after thawing, dilution in various extenders and subsequent cooled storage for 24h, (2) heterologous oocyt...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: J. L ühr, H. Oldenhof, J. Bigalk, G. Martinsson, D. Burger, H. Sieme Source Type: research

In Vitro Evaluation and Fertility of Stallion Frozen Semen
The relationship between post-thaw stallion sperm quality and fertility has been previously studied using a variety of assays. Acrosome Responsiveness Assay (ARA), directed at testing the acrosome ability to react when stimulated with the Ca2+ ionophore A23187, was included in the panel of analysis routinely performed in our laboratory. The ability of fresh sperm to acrosome react when exposed to a capacitating stimulus is lower in subfertile than in fertile stallions. Induction of acrosome reaction has been used in cryopreserved semen to evaluate the fertilizing potential, but results obtained has not been conclusive (Kir...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: B. Mislei, D. Bucci, M. Spinaci, C.C. Love, G. Mari Source Type: research

Reactive Oxygen Species May Be Involved in the Signaling of Equine Sperm Chemotaxis
To find out the way to the oocyte, spermatozoa may be oriented by chemical cues (e.g., progesterone), transport mechanism called chemotaxis (Eisenbach et al. Reviews Molecular Cell Biology; 2006. 4:276-285). Only capacitated spermatozoa can orient their movement by chemotaxis, where part of the signaling (at least in humans) is mediated by the AC-cAMP-PKA pathway (Teves et al. PLoS ONE; 2009. 12: e8211) and oxidative state (Sanchez et al. Fertility and Sterility; 2010. 1:150-153). The aim of this work was to verify whether reactive oxidative species (ROS) are also involved in the chemotaxis signaling and by which mechanism...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: A. Moreno-Irusta, E.M. Dom ínguez, L. Losinno, L. Giojalas Source Type: research

The Effects of Nanoparticle Semen Purification on Semen Quality Parameters in Stallions
This study evaluated the effect of magnetic nanoparticles (NP) coated with i) lectin peanut agglutinin to bind glycans exposed by acrosomal damage (Lectin) and ii) gender specific factors to select for X-sperm population (Gender X) on semen quality parameters. Fresh semen was collected (N=14 stallions) and diluted with Botusemen ®. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: L.H. Morris, T. de Haan, L.G. Landriscina, S. Wilsher, Z. Gibb Source Type: research

Comparison of Whole and Centrifuged Egg-Yolk Added to Kenney ’s and Lactose-EDTA Extenders for Donkey Semen Cryopreservation
Artificial insemination (AI) is the reproductive technique that most influences animal production and using frozen semen presents numerous advantages such as conservation, commercialization and ease of distribution of desirable genes, while allowing sanitary control of semen samples. Although whole egg-yolk (EY) has been the component most widely used as the external cryoprotectant for donkey semen extenders with any degree of success, among its disadvantages is that the large granular material and debris it provides may interfere with sperm metabolism and with semen evaluation as it is of the same size and shape as sperm....
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: A.A. Ferrante, D.M. Neild, C.C. Arraztoa, C. Baca Castex, S. Bruno, J. Plaza, M.H. Miragaya Source Type: research

Fractionated Semen Collection Improved Fertility of Stallions With Seminal Vesiculitis
Seminal vesiculitis is one of the causes of pyospermia affecting the fertility of stallions. To date, recommended treatments have variable results and reoccurrence is a common sequela. The development of management tools for stallions affected is warranted. Thus, the objective of this study was to evaluate fractionated semen collection as a method to rescue fertility of stallions diagnosed with seminal vesiculitis. The study was performed from April of 2014 to October of 2017 with six light-breed stallions with a history of infertility and pyospermia. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: S.N. Oliveira, L.R.P. Andrade, E.A.B. Araujo, L.F.M. Silva, R.M. Rayashi, M.A. Alvarenga, C.P.F. Dell ’Aqua, J.A. Dell’Aqua, I.F. Canisso, P.M. Papa, F.O. Papa Source Type: research

Rosiglitazone Phosphorylates Akt, Reduces Caspase 3 Activity and Enhances Mitochondrial Function in frozen Thawed Stallion Spermatozoa
Spermatozoa surviving freezing - thawing, experience changes that represent an acceleration of the default apoptotic dead pathway that the spermatozoa experience after ejaculation[1]. One key protein related to survival is the Akt, provided that this kinase is phosphorylated (p-Akt), spermatozoa remain viable, but upon dephosphorylation of Akt pro-caspase 3 is cleaved and spermatozoa rapidly lose motility[2]. Cryopreserved semen show impaired mitochondrial function due to oxidative stress and the osmotic damage occurring at thawing[3]. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: M. Ortiz- Rodriguez, C. Ortega Ferrusola, M.C. Gil, F.J. Pe ña Source Type: research

EHV-1 Infection of the Equine Male Genital Tract
Equid Herpesvirus (EHV-1) causes respiratory tract infections in horse populations world-wide. In addition, infection is associated with herd outbreaks of myeloencephalopathy and abortion following a cell-associated viremia. EHV-1 also crosses the blood-testis barrier and is shed with semen in the intact male, lasting typically for several weeks following viremia. As barrier damage is key to myeloencephalopathy and abortion, specific aims of this study were to investigate whether virus can be detected in gonads and/or accessory sex glands of intact male horses 1 month after infection, and if there is evidence of tissue pat...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: C. Otzdorff, K. Gie ßler, C. Haupt, S. Samoilowa, G Soboll Hussey, M. Kiupel, K. Matiasek, L. Goehring Source Type: research

Quercetin Promotes Increase in the Fertility Rate of Frozen Semen of Stallions Considered Sensitive to Freezing
In the equine species the stallions are selected according to their athletic performance or phenotypic characteristics, not taking their reproductive capacity into account in most cases. This fact results in an enormous variation of individuals with resistant or sensitive semen to sperm freezing, being more or less affected by the physical and chemical damages that occur during the use of this biotechnology and by the consequent elevation of the levels of reactive oxygen species (ROS). Quercetin is an antioxidant of the flavonoid group, capable of stabilizing the most ROS and reducing the occurrence of lipid peroxidation. ...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: L.F.M.C. Silva, E.A.B. Araujo, S.N. Oliveira, F.M. Dalanezi, LRP Andrade Junior, J.A.M. Carneiro, L.T. Rodrigues, R.M. Hayashi, A.M. Crespilho, C.P.F. Dell ’Aqua, J.A. Dell’Aqua Junior, F.O. Papa Source Type: research

On-field Electro-ejaculation of Wild Prezwalski Horses: Results and Perspectives
This report aimed to describe an effective and safe on-field electro-ejaculation method and parameters of semen obtained with this method. Fertility of 3 Prezwalski horses was evaluated in a safari park. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: G. Rigaux, S. Parrilla-Hernandez, S. Egyptien, C. Gatez, C. Carrasco-Leroy, S. Deleuze, J. Ponthier Source Type: research