How to Perform Umbilical Cord Arterial and Venous Blood Sampling in Neonatal Foals
Umbilical cord arterial and venous blood gas analysis is a commonly performed procedure in human neonatal medicine to help ascertain a newborn infant ’s oxygenation and acid-base status prior to birth. Defined protocols for performing the procedure have been described in the medical literature. The aim of this report was to describe in detail the procedure for collecting paired blood samples from the umbilical artery and vein in newborn foals s o that stall-side blood gas analysis could be carried out. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 15, 2018 Category: Veterinary Research Authors: Sunita S. Jeawon, Lisa M. Katz, Noreen P. Galvin, Stephen D. Cahalan, Vivienne E. Duggan Tags: Clinical Technique Source Type: research

Extracellular trap formation by donkey polymorphonuclear neutrophils against Toxoplasma gondii
Toxoplasma (T.) gondii is an obligatory intracellular apicomlexan parasite. The donkey is one of the intermediate hosts of T. gondii. There are almost no data about T. gondii infection in donkeys, apart from a few seroprevalence reports. The aim of the study was to detect the formation of extracellular traps by donkey polymorphonuclear neutrophils (PMNs) when exposed to T. gondii tachyzoites in vitro. Tachyzoites were observed to be entrapped within extracellular traps (NET) structures. Myeloperoxidase, neutrophil elastase (NE) and histone (H3) were observed in NET structures. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 15, 2018 Category: Veterinary Research Authors: Kader Yildiz, Sami Gokpinar, Neslihan Sursal, Cahit Babur, Dogukan Ozen, Ahmet Kursat Azkur Source Type: research

Post-thaw addition of autologous seminal plasma improves sperm motion characteristics in fair and poor freezer stallions
During semen processing for cryopreservation, the majority of seminal plasma is usually removed, and components with protective effects on sperm may be missing after thawing and within the female reproductive tract. The present study evaluated the effect of post-thaw addition autologous seminal plasma on motion characteristics of stallion sperm with fair (n = 4) or poor (n = 3) freezability. Therefore, pure seminal plasma (group SP1), seminal plasma combined with fresh semen extender (group SP2), or seminal plasma mixed with freezing extender (group SP3) were used to fill 0.5-ml straws and frozen similar to stallion semen....
Source: Journal of Equine Veterinary Science - November 9, 2018 Category: Veterinary Research Authors: Stefanie Neuhauser, Patricia G ösele, Johannes Handler Source Type: research

Preliminary observations of the effect of garlic on egg shedding in horses naturally infected by intestinal strongyles
Intestinal strongyles are the most common endoparasites of horses and anthelmintic treatments are the main strategy to control these nematodes. However, the development of anthelmintic resistance has led to a decreased efficacy of synthetic drugs and for this reason there is a growing interest in alternative control strategies as the use of medicinal plants. The aim of the present study was to determine the in vivo efficacy of garlic (Allium sativum) in horses naturally infected by intestinal strongyles. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 3, 2018 Category: Veterinary Research Authors: F. Buono, L. Pacifico, D. Piantedosi, G. Sgroi, B. Neola, C. Roncoroni, A. Genovese, D. Rufrano, V. Veneziano Source Type: research

Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System
The aim of our study was to investigate the effect of two freezing extenders and two packaging systems on motility, plasma membrane integrity and the apoptotic status of frozen-thawed (F-T) spermatozoa of the endangered Old Kladruber stallions.The collected semen (n = 6 stallions, three collections each) was diluted either with Gent or Lactose-EDTA (Lact) extender. Two aliquots of semen from each collection diluted in this way were prepared and then loaded into 5 ml aluminium tubes or 0.5 ml plastic straws. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 3, 2018 Category: Veterinary Research Authors: Ji ří Šichtař, Ondřej Šimoník, Filipa Bubeníčková, Jitka Svobodová, Anežka Nehasilová Source Type: research

Glucose and insulin response of aged horses grazing alfalfa, perennial cool-season grass, and teff during the spring and late-fall
Spring and late-fall grazing can lead to metabolic problems in horses (Equus caballus L.) as a result of elevated nonstructural carbohydrates (NSC) in pastures. Therefore, the objectives were to determine the impact of different forage species on blood glucose and insulin concentrations of horses during the spring and late-fall. Research was conducted in May (spring) and October (late-fall) in St. Paul, MN. Alfalfa (Medicago sativa L.) and mixed perennial cool-season grasses (CSG) were grazed in spring, and CSG and teff (Eragrostis tef [Zucc.] Trotter) were grazed in late-fall by six adult horses randomly assigned to a for...
Source: Journal of Equine Veterinary Science - November 3, 2018 Category: Veterinary Research Authors: M.L. DeBoer, M.R. Hathaway, P.S.D. Weber, C.C. Sheaffer, K.J. Kuhle, K.L. Martinson Tags: Short Communication Source Type: research

Determination of phosphorus and nitrogen environmental load from six different bedding types used in an equine facility
The objective of this study was to determine the environmental load of phosphorus (P) and nitrogen (N) from six different bedding materials used in an equine facility. Six horses were housed on six bedding materials — peat moss (PM), wood shavings (WS), wood pellets (WP), straw (ST), chopped straw (CS), and corn cob hulls (CC)— for 7 days each in a Latin Square design. Waste was removed once daily, weighed, mixed, and a 10% sample was saved. At the end of each 7-d period, all samples were pooled, mixed, and a representative sample was analyzed for concentration of P and N. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 3, 2018 Category: Veterinary Research Authors: A.D. Woodward, B.D. Nielsen, A. Pritchard, C.I. O ’Connor-Robison Source Type: research

Foal-level risk factors associated with development of Rhodococcus equi pneumonia at a Quarter Horse breeding farm
The occurrence of R. equi at farms varies, with disease occurring endemically at some farms, but only sporadically, or not at all at other farms. Only some foals residing on endemic farms develop clinical signs of disease. Limited evidence is available regarding foal-level risk factors for development of R. equi pneumonia. The purpose of this study was to identify foal-level risk factors associated with the development of R. equi pneumonia among foals at a large breeding farm in Texas with a recurrent problem of R. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 3, 2018 Category: Veterinary Research Authors: Michelle C. Coleman, Glen P. Blodgett, Kari E. Bevevino, Renata Ivanek, Kevin J. Cummings, G. Kent Carter, Noah D. Cohen Source Type: research

Pharmacokinetics of metformin in combination with sitagliptin in adult horses after enteral administration
The objective of the present study was to establish the plasma concentration –time profile and to derive pharmacokinetics data for a combination of metformin and sitagliptin in horses after enteral administration. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 2, 2018 Category: Veterinary Research Authors: Carlos M. C árceles-Rodríguez, Emilio Fernández-Varón, Tamara Martín-Gimenez, Carla Aguirre, Alexandra Arion, María J. Rodríguez, Ignacio Ayala Source Type: research

Evaluation of the effects of chiropractic on static and dynamic muscle variables in sport horses
– the objective of this prospective controlled experimental study was to assess the effect of chiropractic on static bioimpedance (BI) and dynamic acoustic myography (AMG) of paired muscle groups in healthy sport horses. BI measures the passage of current through muscle and gives information relat ing to the degree of contraction, while AMG records and analyzes low frequency sounds created during muscular activity [1, 2]. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - November 2, 2018 Category: Veterinary Research Authors: Elizabeth V. Acutt, Sarah S. le Jeune, Bruno H. Pypendop Source Type: research

Response to: “Concern Regarding the Publication by Posbergh et al”
Much of the reviewer's concern derives from changes that were made to the article to shorten the overall length. We do not assert that the investigated change is causative of equine degenerative myeloencephalopathy (EDM) disease. However, we do have an ethical obligation to report our statistically significant findings in the hope that it can inform further studies of this condition. It is unfortunate that the reviewer chose to withhold these specific concerns during the review process, instead preferring a public rebuttal to air his criticisms. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - October 29, 2018 Category: Veterinary Research Authors: Christian J. Posbergh, Geoffrey E. Pollott, Teresa L. Southard, Thomas J. Divers, Samantha A. Brooks Tags: Response to Letter to the Editor Source Type: research

Concern Regarding the Publication by Posbergh et  al. “A Nonsynonymous Change in Adhesion G Protein–Coupled Receptor L3 Associated With Risk for Equine Degenerative Myeloencephalopathy in the Caspian Horse,” J Equine Vet Sci 2018;70:96–100
Posbergh et  al (2018) reported on the analysis of equine degenerative myeloencephalopathy in Caspian horses [1]. The authors hypothesized a monogenic autosomal recessive inheritance for this trait. Only two cases were used for the analysis. The cases represented paternal half-siblings and were described as a Caspian and a Caspian cross. The dams were not clearly identified in the publication. However, the authors mention a Shetland Pony, which had an affected offspring. If the Shetland Pony indeed was the dam of one of the two cases of the publication, this would argue against autozygosity in the cases. (Source: Journal ...
Source: Journal of Equine Veterinary Science - October 29, 2018 Category: Veterinary Research Authors: Tosso Leeb Tags: Letter to the Editor Source Type: research

Concern regarding the publication by Posbergh et al. “A nonsynonymous change in adhesion G protein-coupled receptor L3 associated with risk for equine degenerative myeloencephalopathy in the Caspian horse”, J Equine Vet Sci 2018;70:96-100
Posbergh et al. (2018) reported on the analysis of equine degenerative myeloencephalopathy in Caspian horses [1]. The authors hypothesized a monogenic autosomal recessive inheritance for this trait. Only two cases were used for the analysis. The cases represented paternal half-siblings and were described as a Caspian and a Caspian cross. The dams were not clearly identified in the publication. However, the authors mention a Shetland Pony, which had an affected offspring. If the Shetland Pony indeed was the dam of one of the two cases of the publication, this would argue against autozygosity in the cases. (Source: Journal o...
Source: Journal of Equine Veterinary Science - October 29, 2018 Category: Veterinary Research Authors: Tosso Leeb Source Type: research

Response to: “Concern regarding the publication by Posbergh et al....”
Much of the reviewer ’s concern derives from changes that were made to the manuscript to shorten the overall length. We do not assert that the investigated change is causative of EDM disease. However, we do have an ethical obligation to report our statistically significant findings in the hope that it can inform furth er studies of this condition. It is unfortunate that the reviewer chose to withhold these specific concerns during the review process, instead preferring a public rebuttal to air his criticisms. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - October 29, 2018 Category: Veterinary Research Authors: Christian J. Posbergh, Geoffrey E. Pollott, Teresa L. Southard, Thomas J. Divers, Samantha A. Brooks Source Type: research

Cooling And Cryopreservation Of Equine Platelet-Rich Plasma With Dimethyl Sulfoxide And Trehalose
Equine platelet-rich plasma (PRP) has been used in horses to repair bone, articular and tendinous lesions, laminitis, and even endometritis. However, platelets have a very limited lifespan which makes it difficult to prepare and use PRP, except in loco. With the aim to produce PRP with higher platelet viability for clinical purposes, the effects of the cryoprotectants dimethyl sulfoxide (DMSO) and trehalose were evaluated on cooled (4oC) and cryopreserved (-196 °C) equine PRP. The protocols of cooling and cryopreservation were performed independently, comparing the following treatments: fresh PRP, PRP+6%DMSO, PRP+300Tre, ...
Source: Journal of Equine Veterinary Science - October 26, 2018 Category: Veterinary Research Authors: Liomara Andressa do Amaral Kwirant, Fl ávio Desessards De La Corte, Camila Cantarelli, Juliana Felipetto Cargnelutti, Mathias Martins, Mariê Wolski Cabral, Nayrema Maciel, Mara Iolanda Batistella Rubin Tags: Original Research Source Type: research