Cooling And Cryopreservation Of Equine Platelet-Rich Plasma With Dimethyl Sulfoxide And Trehalose

Equine platelet-rich plasma (PRP) has been used in horses to repair bone, articular and tendinous lesions, laminitis, and even endometritis. However, platelets have a very limited lifespan which makes it difficult to prepare and use PRP, except in loco. With the aim to produce PRP with higher platelet viability for clinical purposes, the effects of the cryoprotectants dimethyl sulfoxide (DMSO) and trehalose were evaluated on cooled (4oC) and cryopreserved (-196 °C) equine PRP. The protocols of cooling and cryopreservation were performed independently, comparing the following treatments: fresh PRP, PRP+6%DMSO, PRP+300Tre, and PRP only.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Tags: Original Research Source Type: research