Journal of Equine Veterinary Science Journal of Equine Veterinary Science RSS feedThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Skim Milk, Casein Based Extender or Botucrio in Cooled Semen Asinus (Equus asinus)
The objective of this study was to evaluate semen characteristics after cooling of the semen for 24h in different diluents. Ten ejaculates of different Pega donkeys were used, ranging from four to ten years old. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: L.T. Rodrigues, L.E.F. Canuto, S.N. Oliveira, L.R.P. Andrade, M.A. Alvarenga, J.A. Dell ’Aqua, I.F. Canisso, F.O. Papa Source Type: research

Post-thaw Addition of Caffeine and/or Pentoxifylline Affect Differently Motility Characteristics of Horse and Donkey Cryopreserved Spermatozoa
Post-thaw sperm motility and viability can be poor and thus partially responsible for the low fertility results after AI. In order to improve sperm survival during freezing, equine and donkey semen extenders have been supplemented with a variety of substances: different cryoprotectants, antioxidants, and cholesterol loaded cyclodextrins (e.g. 1-5). Moreover, with respect of equine semen, caffeine and pentoxifylline, two methylxantine derivative phosphodiesterase inhibitors, have been also tested. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: A. Rota, C. Sabatini, A. Przyby ł, A. Ciaramelli, D. Panzani, F. Camillo Source Type: research

Sperm Quality of Stallions Treated With Pentoxifylline After Scrotal Heat Stress
Pentoxifylline has been used in the treatment of rats and men with severe testicular abnormalities, improving their sperm quality and fertility; however, the benefits of this drug have been poorly investigated in the stallion. Therefore, our study aimed to evaluate the effect of oral treatment with pentoxifylline on the seminal quality of stallions after scrotal heat stress. Fourteen healthy stallions with a median age of 6 years were randomly divided into three groups: control (CT, n = 4), degenerate (DG, n = 5) and degenerate treated with pentoxifylline (PTX, n = 5). (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: Y.F.R. Sancler-Silva, B.A. Ball, A. Esteller-Vico, E.R. Silva-J únior, C.P. Freitas-Dell’aqua, F.O. Papa Source Type: research

Effect of Sampling Techniques on Enumeration of Equine Testicular Cell Types by Flow Cytometry
Direct evaluation of stallion testicular parenchyma though biopsy is an uncommon procedure due to the concern about associated trauma. Diagnosis of testicular pathology and measurement of spermatogenic efficiency may be more applicable if a less invasive technique, needle aspiration, is used to acquire testicular tissue sample and analyze by flow cytometry. Sampling techniques (i.e., tissue location; storage time, and acquisition method) may affect testicular cell types. Percentages of different spermatogenic cell types were evaluated: 4n (young [YN] and old [OD] primary spermatocytes), 2n (secondary spermatocytes [SS], sp...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: R. Serafini, D.D. Varner, C. Hern ández-Avilés, S.R. Teague, K.A. LaCaze, C.C. Love Source Type: research

Approach to the Study of the Relationship Between DNA Damage and Oxidative Stress in Equine Semen
Reactive oxygen species (ROS) are produced by living organisms as a result of normal cellular metabolism and play beneficial roles in the innate immune response or cellular stress protection among others. However, ROS can cause damage to cellular components such as lipids, proteins or DNA. Oxidative stress is the result of an imbalance between ROS production and total antioxidant capacity. Spermatozoa and seminal plasma (SP) have several enzymes and other antioxidants to neutralize ROS. In the equine, association between oxidative stress, antioxidant protection and sperm DNA damage has not been studied in detail. (Source: ...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: G. Varga, C. Serres, J. Gos álvez, J. Bartolomé, F. Crespo Source Type: research

Unraveling Infertility: Deciphering the Molecular Basis of Idiopathic Infertility in a Thoroughbred Stallion
In this study we report on the investigation of complete infertility in a 5-year-old Thoroughbred stallion. The stallion was considered infertile after performing 72 covers and achieving 0 pregnancies over one breeding season. Although initial semen assessment (sperm concentration, motility, longevity and morphology) revealed below average parameters, these were not sufficient to explain complete infertility under a managed system. The stallion displayed normal libido, breeding behavior and ejaculation, and semen contained viable, progressively motile spermatozoa (number of total progressively motile sperm per ejaculate ra...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: A. Swegen, J.R. Clulow, M. Baker, R.J. Aitken, Q.T. Tram, Z. Gibb Source Type: research

Effect of Embryo-Recipient Synchrony on Post-ET Survival of In Vivo and In Vitro-Produced Equine Embryos
Equine embryo transfer (ET) permits a relatively large degree of ovulation asynchrony between donor and recipient mare (+1 to –4 days). However, when the embryo is more than 4 days advanced than the uterus, its development is retarded after ET [1]; conversely, pregnancy rarely results in recipients that ovulate ≥2 days before the donor [2]. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: J. Cuervo-Arango, A.N. Claes, T.A. Stout Source Type: research

Day 8 Embryos and Endometrium From Aluteal Cycles in Mares Exhibit Differential Gene Expression
Aluteal (AL) cycles were induced to evaluate the effects of progesterone-deprivation on gene expression of in vivo preimplantation embryos and endometrium. We hypothesized that transcript expression would be altered during AL cycles (progesterone 4 ng/mL). (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: C.A. Leisinger, C. Klein, M.L. Markle, C. Premanandan, J.L. Sones, C.R.F. Pinto, D.L. Paccamonti Source Type: research

Mare and Stallion Effects on Blastocyst Production in a Commercial OPU-ICSI Program
This study retrospectively examined the influence of mare and stallion on the success of OPU-ICSI, in terms of blastocyst production per treatment cycle. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: T.A. Stout, A. Claes, S. Colleoni, G. Lazzari, C. Galli, J. Cuervo-Arango Source Type: research

Effect of Artificial Activation of Equine Oocytes on Cleavage and Blastocyst Production Following ICSI
In this study we performed ICSI on in vitro-matured oocytes using frozen semen that had exhibited poor cleavage and blastocyst production in a clinical ICSI program, and compared three methods of artificial oocyte activation. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: R.R. Foss, H.A. Ortis, K.D. Loncar Source Type: research

Effect of Warming Method in a Simplified Equine Embryo Vitrification System
Embryo cryopreservation has the potential to be an important tool in equine reproduction. Several studies have reported successful equine embryo vitrification, but most of these have utilized warming by experienced personnel, in the vitrification laboratory. Our goal was to develop an effective embryo vitrification protocol in which the practitioner could easily warm the embryo at the transfer facility, for immediate transfer. We vitrified in vitro-produced (IVP) blastocysts using commercial in-straw embryo vitrification media (EquiPro Vit Kit, MOFA Global, Verona, USA), with the Cryolock device (Biotech Inc., Alpharetta G...
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: H.S. Canesin, A.N. Rocha Filho, R.M. Salgado, I. Ortiz, J.G. Brom-de-Luna, K. Hinrichs Source Type: research

Prolonged In Vitro Maturation Time and Increased Intensity of Piezo Pulses During ICSI Enhance Cleavage and Embryo Development in the Horse
The use of assisted reproduction techniques in horses is increasing, in particular in vitro embryo production by OPU and ICSI. Compared to cattle the percentage of oocytes that become transferable blastocysts is lower both under experimental [1] and clinical settings [2]. The horse has peculiar features, showing a varied population of immature oocytes that require different timing for reaching both nuclear and cytoplasmic maturation. Immature oocytes can also be put on hold at room temperature with no loss of viability [3]. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: C. Galli, S. Colleoni, P. Turini, G. Crotti, G. Lazzari Source Type: research

Proteomic Analysis of Pregnant Mare Uterine Fluid
The maternal recognition of pregnancy (MRP) is the physiological process whereby the conceptus signals its presence to its maternal host. From approximately day 6 to day 16 after fertilization the equine conceptus continually migrates throughout the uterus. It contacts the endometrium during this migration but relies exclusively on the uterine fluid for nourishment and survival. It is anticipated that throughout this 10-day passage the yet unknown message triggering MRP in the mare may pass through the fluid. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: E.F. Lawson, Z. Gibb, M. de Ruijter-Villani, N.D. Smith, T.A. Stout, A. Clutton-Brock, J.R. Aitken, A. Swegen Source Type: research

Proteomic Profiling of Equine Blastocoel Fluid and Functional Mapping of Embryo Transcriptome
Early pregnancy in horses is a critical period during which the conceptus shows several characteristic differences from that of other domestic animals. The conceptus communicates with the oviduct and unfertilized ova fail to descend into the uterus. The conceptus forms a glycoproteinaceous capsule, remains highly mobile and continues to increase in size. The blastocoel fluid accumulated during this rapid growth may contain proteins that signal further development of the conceptus and promote interactions with the uterine microenvironment for the sustenance of pregnancy. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: V.V. Pillai, A.W. Herren, B.S. Phinney, J.M. Castillo, M. Diel de Amorim, V. Selvaraj, S.H. Cheong Source Type: research

Functional Annotation of Genes Expressed in the Developing Equine Placenta
The full equine genome sequence, now freely available to researchers worldwide, has dramatically advanced equine research in many areas. However, although the protein coding genes of the horse are now quite well defined, the non-coding elements that control gene transcription and other aspects of gene expression remain largely uncharacterized and are poorly understood. Such information is crucial to exploiting the full potential of genomics to advance equine research, including applications in reproduction. (Source: Journal of Equine Veterinary Science)
Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: D.F. Antczak, D.C. Miller, E.J. Rice, C.G. Danko Source Type: research