Effect of Sampling Techniques on Enumeration of Equine Testicular Cell Types by Flow Cytometry

Direct evaluation of stallion testicular parenchyma though biopsy is an uncommon procedure due to the concern about associated trauma. Diagnosis of testicular pathology and measurement of spermatogenic efficiency may be more applicable if a less invasive technique, needle aspiration, is used to acquire testicular tissue sample and analyze by flow cytometry. Sampling techniques (i.e., tissue location; storage time, and acquisition method) may affect testicular cell types. Percentages of different spermatogenic cell types were evaluated: 4n (young [YN] and old [OD] primary spermatocytes), 2n (secondary spermatocytes [SS], spermatogonia, and somatic cells [SC]) and 1n (round [RD] and elongating/elongated [EL] spermatids).
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research