Effect of Warming Method in a Simplified Equine Embryo Vitrification System

Embryo cryopreservation has the potential to be an important tool in equine reproduction. Several studies have reported successful equine embryo vitrification, but most of these have utilized warming by experienced personnel, in the vitrification laboratory. Our goal was to develop an effective embryo vitrification protocol in which the practitioner could easily warm the embryo at the transfer facility, for immediate transfer. We vitrified in vitro-produced (IVP) blastocysts using commercial in-straw embryo vitrification media (EquiPro Vit Kit, MOFA Global, Verona, USA), with the Cryolock device (Biotech Inc., Alpharetta GA, USA).

Source: Journal of Equine Veterinary Science - June 23, 2018 Category: Veterinary Research Authors: H.S. Canesin, A.N. Rocha Filho, R.M. Salgado, I. Ortiz, J.G. Brom-de-Luna, K. Hinrichs Source Type: research