Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System
The aim of our study was to investigate the effect of two freezing extenders and two packaging systems on motility, plasma membrane integrity and the apoptotic status of frozen-thawed (F-T) spermatozoa of the endangered Old Kladruber stallions.The collected semen (n = 6 stallions, three collections each) was diluted either with Gent or Lactose-EDTA (Lact) extender. Two aliquots of semen from each collection diluted in this way were prepared and then loaded into 5 ml aluminium tubes or 0.5 ml plastic straws.
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Ji ří Šichtař, Ondřej Šimoník, Filipa Bubeníčková, Jitka Svobodová, Anežka Nehasilová Source Type: research