Comparison of Two Methods for Stallion Sperm Vitrification

Sperm vitrification is based on high-speed cooling by direct plunging of small volumes of sperm into liquid nitrogen without any permeable cryoprotectant, adding instead carbohydrates and proteins. This technique could be performed with direct contact of the sperm with liquid nitrogen (non-aseptic technique) or using straws that allows the isolation of the sperm from liquid nitrogen, therefore reducing the potential risk of microbial contamination (Isachenko et al. Andrology. 2017; 5; 1055-1063).
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research