In Vitro Evaluation and Fertility of Stallion Frozen Semen

The relationship between post-thaw stallion sperm quality and fertility has been previously studied using a variety of assays. Acrosome Responsiveness Assay (ARA), directed at testing the acrosome ability to react when stimulated with the Ca2+ ionophore A23187, was included in the panel of analysis routinely performed in our laboratory. The ability of fresh sperm to acrosome react when exposed to a capacitating stimulus is lower in subfertile than in fertile stallions. Induction of acrosome reaction has been used in cryopreserved semen to evaluate the fertilizing potential, but results obtained has not been conclusive (Kirk et al Theriogenology 2005 64,1422-1429, Barrier Battut et al Theriogenology 2017 97, 186-200).
Source: Journal of Equine Veterinary Science - Category: Veterinary Research Authors: Source Type: research