Supplementation of metabolizable protein during late gestation and fetal number impact ewe organ mass, maternal serum hormone and metabolite concentrations, and conceptus measurements
To examine the effects of maternal metabolizable protein (MP) supplementation during late gestation on serum hormone and metabolites and organ masses, multiparous ewes (n = 45) carrying singletons or twins were allotted randomly (within pregnancy group) to 1 of 3 treatments: 60% (MP60), 80% (MP80), or 100% (MP100) of MP requirements. Blood samples were drawn before the initiation of diets (day 100) and before slaughter (day 130) for chemistry panel analysis and weekly for hormone analysis including progesterone (P4) and estradiol-17β (E2). (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - August 25, 2015 Category: Endocrinology Authors: T.J. Swanson, L.A. Lekatz, M.L. Van Emon, G.A. Perry, C.S. Schauer, K.R. Maddock Carlin, C.J. Hammer, K.A. Vonnahme Source Type: research
Studies on lysophosphatidic acid action during in vitro preimplantation embryo development
Assisted reproductive technologies, including in vitro embryo production (IVP), have been successfully used in animal reproduction to optimize breeding strategies for improved production and health in animal husbandry. Despite the progress in IVP techniques over the years, further improvements in in vitro embryo culture systems are required for the enhancement of oocyte and embryo developmental competence. One of the most important issues associated with IVP procedures is the optimization of the in vitro culture of oocytes and embryos. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - August 25, 2015 Category: Endocrinology Authors: D. Boruszewska, E. Sinderewicz, I. Kowalczyk-Zieba, K. Grycmacher, I. Woclawek-Potocka Source Type: research
Supplementation of metabolizable protein during late gestation and fetal number impacts ewe organ mass, maternal serum hormone and metabolite concentrations, and conceptus measurements
To examine the effects of maternal metabolizable protein (MP) supplementation during late gestation on serum hormone and metabolites and organ masses, multiparous ewes (n = 45) carrying singletons or twins were allotted randomly (within pregnancy group) to one of three treatments: 60% (MP60), 80% (MP80), or 100% (MP100) of MP requirements. Blood samples were drawn before initiation of diets (day 100) and prior to slaughter (day 130) for chemistry panel analysis, and weekly for hormone analysis including progesterone (P4) and estradiol-17β (E2). (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - August 25, 2015 Category: Endocrinology Authors: T.J. Swanson, L.A. Lekatz, M.L. Van Emon, G.A. Perry, C.S. Schauer, K.R. Maddock Carlin, C.J. Hammer, K.A. Vonnahme Source Type: research
Studies on lysophosphatidic acid action during preimplantation embryo development
Assisted reproductive technologies (ART), including in vitro embryo production (IVP), have been successfully used in animal reproduction to optimize breeding strategies for improved production and health in animal husbandry. Despite the progress in IVP techniques over the years, further improvements in in vitro embryo culture systems are required for the enhancement of oocyte and embryo developmental competence. One of the most important issues associated with IVP procedures is the optimization of the in vitro culture of oocytes and embryos. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - August 25, 2015 Category: Endocrinology Authors: D. Boruszewska, E. Sinderewicz, I. Kowalczyk-Zieba, K. Grycmacher, I. Woclawek-Potocka Source Type: research
Supplementation of metabolizable protein during late gestation and fetal number impact ewe organ mass, maternal serum hormone and metabolite concentrations, and conceptus measurements
To examine the effects of maternal metabolizable protein (MP) supplementation during late gestation on serum hormone and metabolites and organ masses, multiparous ewes (n = 45) carrying singletons or twins were allotted randomly (within pregnancy group) to 1 of 3 treatments: 60% (MP60), 80% (MP80), or 100% (MP100) of MP requirements. Blood samples were drawn before the initiation of diets (day 100) and before slaughter (day 130) for chemistry panel analysis and wee kly for hormone analysis including progesterone (P4) and estradiol-17β (E2). (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - August 24, 2015 Category: Endocrinology Authors: T.J. Swanson, L.A. Lekatz, M.L. Van Emon, G.A. Perry, C.S. Schauer, K.R. Maddock Carlin, C.J. Hammer, K.A. Vonnahme Source Type: research
Plasma anti-Müllerian hormone as a predictive endocrine marker to select (Holstein) and (Nelore) calves for embryo production
This study evaluated the association between plasma anti-Müllerian hormone (AMH) concentrations and in vitro embryo production (IVEP) in Bos indicus (Nelore; Experiment 1) and Bos taurus (Holstein; Experiment 2) calves superstimulated or not with 140 mg of porcine follicle-stimulating hormone (pFSH; four decreasing doses twice daily). Oocytes were recovered from calves aged 2 to 4 mo after receiving gonadotropin stimulation (Nelore: n=15; Holstein: n=12) or not (Nelore: n=15; Holstein: n=12). Cycling heifers formed a positive control group [n=15 for Nelore (aged 18 to 24 mo); n=10 for Holstein (aged 14 to 16 mo)]. (Source...
Source: Domestic Animal Endocrinology - August 8, 2015 Category: Endocrinology Authors: E.O.S. Batista, B.M. Guerreiro, B.G. Freitas, J.C.B. Silva, L.M. Vieira, R.M. Ferreira, R.G. Resende, A.C. Basso, R.N.V.R. Lopes, F.P. Rennó, A.H. Souza, P.S. Baruselli Source Type: research
Potential role for GnRH in the synchronization of follicular emergence before the superovulatory Day 0 protocol
The ability of GnRH to synchronize ovulation and new follicular wave emergence before a “superovulatory Day 0” protocol was assessed in Santa Inês ewes. For estrus synchronization, a 60-mg medroxyprogesterone acetate sponge was inserted for 6 d. One day before sponge removal, 37.5-μg d-cloprostenol and 300 IU eCG were injected intramuscularly (i.m.). After sponge removal, ewes were assigned to the following 3 groups: (1) GC—1-mL saline at 12 h (n = 10); (2) G24h—0.025-mg lecirelin (GnRH agonist) i.m. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 31, 2015 Category: Endocrinology Authors: M.F.A. Balaro, J.F. Fonseca, T.G.B. Barbosa, J.M.G. Souza-Fabjan, L.M. Figueira, T.A. Teixeira, L.R. Carvalheira, F.Z. Brandão Tags: Short Communication Source Type: research
Potential role for GnRH in the synchronization of follicular emergence prior to the Superovulatory Day 0 protocol
The ability of GnRH to synchronize ovulation and new follicular wave emergence prior to a “Superovulatory Day 0” protocol was assessed in Santa Inês ewes. For oestrus synchronization, a 60-mg medroxyprogesterone acetate sponge was inserted for 6 d. One day before sponge removal, 37.5 μg d-cloprostenol and 300 IU eCG were injected i.m. After sponge removal, ewes were assigned to the following 3 groups: 1) GC – 1 mL saline at 12 h (n = 10), 2) G24h – 0.025 mg lecirelin (GnRH agonist) i.m. at 24 h (n = 10), or 3) G36h – 0.025 mg lecirelin i.m. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 31, 2015 Category: Endocrinology Authors: M.F.A. Balaro, J.F. Fonseca, T.G.B. Barbosa, J.M.G. Souza-Fabjan, L.M. Figueira, T.A. Teixeira, L.R. Carvalheira, F.Z. Brandão Source Type: research
Ovarian and hormonal responses to single or continuous peripheral administration of senktide, a neurokinin 3 receptor agonist, during the follicular phase in goats
The present study aimed to investigate the effects of single or continuous administration of a neurokinin 3 receptor agonist, senktide, on hormonal and follicular dynamics in follicular-phase goats. Goats were injected with PGF2α in the luteal phase, and treated with an intravaginal progesterone device for 10 d. At 12 h after the cessation of progesterone treatment, the goats received a single i.v. injection of senktide (200 nmol, n = 4) or vehicle (n = 4), or continuous i.v. infusion of senktide (20 nmol/min, n = 6) or vehicle (n = 6) for 6 h. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 25, 2015 Category: Endocrinology Authors: Natsumi Endo, Larasati Puji Rahayu, Yasuna Ito, Tomomi Tanaka Source Type: research
Insulin: Pancreatic secretion and adipocyte regulation
Insulin is the primary acute anabolic coordinator of nutrient partitioning. Hyperglycemia is the main stimulant of insulin secretion, but other nutrients such as specific amino acids, fatty acids, and keto-acids can potentiate pancreatic insulin release. Incretins are intestinal hormones with insulinotropic activity and are secreted in response to food ingestion thus integrating diet chemical composition with the regulation of insulin release. In addition, prolactin is required for proper islet development and it stimulates β-cell proliferation. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 18, 2015 Category: Endocrinology Authors: L.H. Baumgard, G.J. Hausman, M.V. Sanz Fernandez Source Type: research
Pharmacologic analyses of four chicken melanocortin-4 receptor mutations
The melanocortin-4 receptor (MC4R) is a critical regulator of mammalian food intake and energy expenditure, with receptor activation resulting in decreased food intake and increased energy expenditure. Recently, studies on role of MC4R in regulation of food intake have been extended to other species, such as chicken. Functional study of mutant MC4Rs is important in proving the causal link between MC4R mutation and production traits. Herein, we cloned chicken MC4R (cMC4R) complementary DNA and generated 4 mutant cMC4Rs (Q18H, G21R, S76L, and L299P) by site-directed mutagenesis and measured their expression by flow cytometr...
Source: Domestic Animal Endocrinology - July 2, 2015 Category: Endocrinology Authors: Z.-Q. Wang, J.-S. Huang, J.-H. Zhou, L. Shi, X.-F. Jiang, Y.-X. Tao Source Type: research
Effects of insulin treatment on the response to oleate and octanoate of food intake and fatty acid–sensing systems in rainbow trout
We hypothesized that food intake and the response of fatty acid (FA)-sensing systems in hypothalamus, liver, and Brockmann bodies of rainbow trout to raised levels of oleate (OL) or octanoate (OCT) is modified by insulin treatment. To assess this hypothesis, 15 fish per group received intraperitoneally 10-mL/kg injection of saline solution alone (control), or containing insulin (2-mg bovine insulin/kg body mass), OL (300 μg/kg), OCT (300 μg/kg), insulin + OL, or insulin + OCT to be sampled 6 h later to assess parameters related to FA sensing. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 2, 2015 Category: Endocrinology Authors: M. Librán-Pérez, C. Velasco, C. Otero-Rodiño, M.A. López-Patiño, J.M. Míguez, J.L. Soengas Source Type: research
Pharmacologic analyses of 4 chicken melanocortin-4 receptor mutations
The melanocortin-4 receptor (MC4R) is a critical regulator of mammalian food intake and energy expenditure, with receptor activation resulting in decreased food intake and increased energy expenditure. Recently, studies on role of MC4R in regulation of food intake have been extended to other species, such as chicken. Functional study of mutant MC4Rs is important in proving the causal link between MC4R mutation and production traits. Herein, we cloned chicken MC4R (cMC4R) complementary DNA and generated 4 mutant cMC4Rs (Q18H, G21R, S76L, and L299P) by site-directed mutagenesis and measured their expression by flow cytometr...
Source: Domestic Animal Endocrinology - July 2, 2015 Category: Endocrinology Authors: Z.-Q. Wang, J.-S. Huang, J.-H. Zhou, S. Lin, X.-F. Jiang, Y.-X. Tao Source Type: research
Pharmacological analyses of four chicken melanocortin-4 receptor mutations
The melanocortin-4 receptor (MC4R) is a critical regulator of mammalian food intake and energy expenditure, with receptor activation resulting in decreased food intake and increased energy expenditure. Recently, studies on role of MC4R in regulation of food intake have been extended to other species, such as chicken. Functional study of mutant MC4Rs is important in proving the causal link between MC4R mutation and production traits. Herein, we cloned chicken MC4R (cMC4R) cDNA and generated four mutant cMC4Rs (Q18H, G21R, S76L, and L299P) by site-directed mutagenesis and measured their expression by flow cytometry. (Source:...
Source: Domestic Animal Endocrinology - July 2, 2015 Category: Endocrinology Authors: Z.-Q. Wang, J.-S. Huang, J.-H. Zhou, S. Lin, X.-F. Jiang, Y.-X. Tao Source Type: research
Effects of insulin treatment on the response to oleate and octanoate of food intake and fatty acid sensing systems in rainbow trout
We hypothesized that food intake and the response of fatty acid (FA)-sensing systems in hypothalamus, liver and Brockmann bodies (BB) of rainbow trout to raised levels of oleate (OL) or octanoate (OCT) is modified by insulin treatment. To assess this hypothesis, 15 fish per group received intraperitoneally 10 mL/kg injection of saline solution alone (control), or containing insulin (2 mg bovine insulin/kg body mass), OL (300 μg/kg), OCT (300 μg/kg), insulin + OL, or insulin + OCT to be sampled 6 h later to assess parameters related to FA sensing. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - July 2, 2015 Category: Endocrinology Authors: M. Librán-Pérez, C. Velasco, C. Otero-Rodiño, M.A. López-Patiño, J.M. Míguez, J.L. Soengas Source Type: research
