Increased expression of pentraxin 3 after in vivo and in vitro stimulation with gonadotropins in porcine oocyte-cumulus complexes and granulosa cells
It has been previously shown that multimeric pentraxin 3 (PTX3) is a key component of the cumulus oophorus extracellular matrix (ECM) in mice. In response to the ovulatory LH surge, the cumulus cells assemble a unique ECM that envelopes the oocyte and cumulus cell complex. Importantly, cumuli from PTX3−/− mice were defective in their ECM organization and their fertility was impaired. It has been demonstrated that tumor necrosis factor alpha–induced protein 6 catalyzes the formation of heavy chains of (inter-alpha-trypsin inhibitor) -hyaluronan complexes and these are then cross-linked via PTX3. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - February 18, 2016 Category: Endocrinology Authors: E. Nagyova, J. Kalous, L. Nemcova Source Type: research
Expression of nerve growth factor and its receptors in the uterus of rabbits: functional involvement in prostaglandin synthesis
The aim of the present study was to evaluate: 1) the presence of nerve growth factor (NGF) and the neurotrophic tyrosine kinase receptor 1 (NTRK1) and nerve growth factor receptor (NGFR) in the rabbit uterus; and 2) the in vitro effects of NGF on PGF2α and PGE2 synthesis and on the PGE2-9-ketoreductase (PGE2-9-K) activity by the rabbit uterus. NGF, NTRK1, and NGFR were immunolocalized in the luminal and glandular epithelium and stroma cells of the endometrium. RT-PCR indicated the presence of mRNA for NGF, NTRK1, and NGFR in the uterus. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - February 18, 2016 Category: Endocrinology Authors: M. Maranesi, F. Parillo, L. Leonardi, P.G. Rebollar, B. Alonso, L. Petrucci, A. Gobbetti, C. Boiti, J. Arruda-Alencar, A. Moura, M. Zerani Source Type: research
Increased expression of pentraxin 3 after in vivo and in vitro stimulation with gonadotropins in porcine oocyte-cumulus complexes and granulosa cells
It has been previously shown that multimeric pentraxin 3 (PTX3) is a key component of the cumulus oophorus extracellular matrix (ECM) in mice. In response to the ovulatory LH surge, the cumulus cells assemble a unique ECM that envelopes the oocyte and cumulus cell complex. Importantly, cumuli from PTX3-/- mice were defective in their ECM organization and their fertility was impaired. It has been demonstrated that tumor necrosis factor alpha-induced protein 6 (TNFAIP6) catalyzes the formation of heavy chains of (inter-alpha-trypsin inhibitor) -hyaluronan complexes and these are then cross-linked via PTX3. (Source: Domestic ...
Source: Domestic Animal Endocrinology - February 18, 2016 Category: Endocrinology Authors: E. Nagyova, J. Kalous, L. Nemcova Source Type: research
Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum
The objectives of the current study were to characterize the tissue expression of chicken (Gallus gallus) bone morphogenetic protein 4 (BMP4) and compare differences in its expression in abdominal fat tissue and serum between fat and lean birds, and to determine a potential relationship between the expression of BMP4 and abdominal fat tissue growth and development. The results showed that chicken BMP4 mRNA and protein were expressed in various tissues, and the expression level of BMP4 transcript and protein was relatively higher in adipose tissues. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - January 29, 2016 Category: Endocrinology Authors: B.H. Cheng, L. Leng, M.Q. Wu, Q. Zhang, X.Y. Zhang, S.S. Xu, Z.P. Cao, Y.M. Li, P. Luan, H. Li Source Type: research
Effects of steroid treatment on growth, nutrient partitioning, and expression of genes related to growth and nutrient metabolism in adult triploid rainbow trout ()
The contribution of sex steroids to nutrient partitioning and energy balance during gonad development was studied in rainbow trout. Specifically, 19-mo old triploid (3N) female rainbow trout were fed treatment diets supplemented with estradiol-17β (E2), testosterone (T), or dihydrotestosterone (DHT) at 30 mg steroid/kg diet for a 1-mo period. Growth performance, nutrient partitioning, and expression of genes central to growth and nutrient metabolism were compared to 3N and age-matched diploid (2N) female fish consuming a control diet not supplemented with steroids. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - January 25, 2016 Category: Endocrinology Authors: B.M. Cleveland, G.M. Weber Source Type: research
Ovarian follicle development in vitro and oocyte competence: advances and challenges for farm animals
During the last 2 decades, research on in vitro preantral follicle growth and oocyte maturation has delivered fascinating advances concerning the knowledge of processes regulating follicle growth and the developmental competence of oocytes. These advances include (1) information about the role of several hormones and growth factors on in vitro activation of primordial follicles; (2) increased understanding of the intracellular pathway involved in the initiation of primordial follicle growth; (3) the growth of primary and secondary follicles up to antral stages; and (4) production of embryos from oocytes from in vitro gr...
Source: Domestic Animal Endocrinology - January 6, 2016 Category: Endocrinology Authors: J.R.V. Silva, R. van den Hurk, J.R. Figueiredo Source Type: research
Ovarian follicle development and oocyte competence: advances and challenges for farm animals
During the last two decades, research on in vitro preantral follicle growth and oocyte maturation has delivered fascinating advances concerning the knowledge of processes regulating follicle growth and the developmental competence of oocytes. These advances include: (1) information about the role of several hormones and growth factors on in vitro activation of primordial follicles; (2) increased understanding of the intracellular pathway involved in the initiation of primordial follicle growth, (3) the growth of primary and secondary follicles up to antral stages, and (4) production of embryos from oocytes from in vitro gr...
Source: Domestic Animal Endocrinology - January 6, 2016 Category: Endocrinology Authors: J.R.V. Silva, R. van den Hurk, J.R. Figueiredo Source Type: research
Leptin concentrations in finishing beef steers and heifers and their association with dry matter intake, average daily gain, feed efficiency, and body composition
The objective of this experiment was to determine the association of circulating plasma leptin concentrations with production and body composition measures of finishing beef steers and heifers and to determine if multiple sampling time points improve the associations of plasma leptin concentrations with production and body composition traits. Individual DMI and ADG were determined for 84 d using steers and heifers (n = 127 steers and n = 109 heifers). Blood was collected on day 0, day 42, and day 83 for determination of plasma leptin concentrations. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - January 6, 2016 Category: Endocrinology Authors: A.P. Foote, R.G. Tait, D.H. Keisler, K.E. Hales, H.C. Freetly Source Type: research
Plasma concentrations of acyl-ghrelin are associated with average daily gain and feeding behavior in grow-finish pigs
The objectives of this study were to determine the effect of sex, sire line, and litter size on concentrations of acyl-ghrelin and total ghrelin in plasma of grow-finish pigs, and to understand the relationship of plasma concentrations of ghrelin with feeding behavior, average daily gain (ADG), and back fat (BF) in grow-finish swine. Yorkshire-Landrace crossbred dams were inseminated with semen from Yorkshire, Landrace, or Duroc sires. Within 24 h of birth, pigs were cross-fostered into litter sizes of normal (N; > 12 pigs/litter) or small (S; ≤ 9 pigs/litter). (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - January 4, 2016 Category: Endocrinology Authors: C.A. Lents, T.M. Brown-Brandl, G.A. Rohrer, W.T. Oliver, B.A. Freking Source Type: research
Concentrations of progesterone, a metabolite of PGF2α, prolactin, and luteinizing hormone during development of idiopathic persistent corpus luteum in mares
In experiment 1, daily blood samples were available from days 0 to 20 (day 0 = ovulation) in mares with an interovulatory interval (IOI, n = 5) and in mares that developed idiopathic persistent corpus luteum (PCL, n = 5). The PCL was confirmed by maintenance of progesterone (P4) concentration until end of the experiment (day 20). Significant interactions of group and day revealed the novel findings that luteinizing hormone (LH) was lower (P < 0.05) in the PCL group than that in the IOI group on days 0 to 4, and prolactin was lower (P < 0.05) on days 1, 4, 6, and 7. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - December 27, 2015 Category: Endocrinology Authors: O.J. Ginther, J.M. Baldrighi, T. Castro, C.A. Wolf, V.G. Santos Source Type: research
Concentrations of progesterone, a metabolite of PGF2α, prolactin, and LH during development of idiopathic persistent corpus luteum in mares
In experiment 1, daily blood samples were available from Days 0 to 20 (Day 0 = ovulation) in mares with an interovulatory interval (IOI, n=5) and in mares that developed idiopathic persistent corpus luteum (PCL, n=5). The PCL was confirmed by maintenance of progesterone (P4) concentration until end of the experiment (Day 20). Significant interactions of group and day revealed the novel findings that LH was lower (P < 0.05) in the PCL group than in the IOI group on Days 0 to 4, and prolactin was lower (P < 0.05) on Days 1, 4, 6, and 7. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - December 27, 2015 Category: Endocrinology Authors: O.J. Ginther, J.M. Baldrighi, T. Castro, C.A. Wolf, V.G. Santos Source Type: research
Aquaporin 3 is regulated by estrogen in the chicken oviduct and is involved in progression of epithelial cell–derived ovarian carcinomas
Aquaporins (AQPs) are membrane proteins that passively deliver water across the plasma membrane to play an important role in maintaining cell shape. Members of the AQP family are distributed in most of the tissues in the human body and perform a variety of functions based on the water homeostasis suitable for each organ. However, there is little known about the expression and regulation of AQP family members in chickens. Therefore, we determined the expression of AQPs in various tissues of chickens. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - December 23, 2015 Category: Endocrinology Authors: C. Yang, W. Lim, H. Bae, G. Song Source Type: research
Aquaporin 3 is regulated by estrogen in the chicken oviduct, and it is involved in progression of epithelial cell–derived ovarian carcinomas
Aquaporins (AQPs) are membrane proteins that passively deliver water across the plasma membrane to play an important role in maintaining cell shape. Members of the AQP family are distributed in most of the tissues in the human body and perform a variety of functions based on the water homeostasis suitable for each organ. However, there is little known about the expression and regulation of AQP family members in chickens. Therefore, we determined the expression of AQPs in various tissues of chickens. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - December 23, 2015 Category: Endocrinology Authors: C. Yang, W. Lim, H. Bae, G. Song Source Type: research
Aquaporin 3 is regulated by estrogen in the chicken oviduct and it is involved in progression of epithelial cell-derived ovarian carcinomas
Aquaporins (AQPs) are membrane proteins that passively deliver water across the plasma membrane to play an important role in maintaining cell shape. Members of the AQP family are distributed in the majority of tissues in the human body and perform a variety of functions based on the water homeostasis suitable for each organ. However, there is little known about expression and regulation of AQP family members in chickens. Therefore, we determined the expression of AQPs in various tissues of chickens. (Source: Domestic Animal Endocrinology)
Source: Domestic Animal Endocrinology - December 23, 2015 Category: Endocrinology Authors: C. Yang, W. Lim, H. Bae, G. Song Source Type: research
Expression and immunolocalization of membrane progesterone receptors in the bovine oviduct
The oviduct plays a crucial role in the transport and maturation of gametes and ensures suitable conditions for fertility and early embryo development. One regulator of oviduct function is progesterone (P4), which affects the cell by interacting with nuclear progesterone receptors (PGR) as well as through non-genomic mechanisms, presumably involving membrane progesterone receptors. The aim of this study was to evaluate expression of mRNAs and proteins for progesterone receptor membrane component (PGRMC) 1 and 2 and membrane progestin receptors (mPR) α, β and γ and to use immunohistochemistry to demonstrate their cell-sp...
Source: Domestic Animal Endocrinology - December 16, 2015 Category: Endocrinology Authors: M.K. Kowalik, M. Martyniak, R. Rekawiecki, J. Kotwica Source Type: research
