Sclerotiorin inhibits protein kinase G from Mycobacterium tuberculosis and impairs mycobacterial growth in macrophages
As a eukaryotic-like Ser/Thr protein kinase, Mycobacterium tuberculosis virulent effector protein kinase G (PknG) mediates mycobacterial survival by regulating bacterial cell metabolic processes and preventing phagosome-lysosome fusion in host macrophages. Targeting PknG is an effective strategy for development of anti-tuberculosis (TB) drugs. In the study, we found that sclerotiorin, derived from marine fungi from the South China Sea, exhibited moderately strong inhibitory effects on recombinant PknG, with an IC50 value of 76.5 μM, and acted as a non-competitive inhibitor. (Source: Tuberculosis)
Source: Tuberculosis - January 13, 2017 Category: Respiratory Medicine Authors: Dongni Chen, Shuangshuang Ma, Lei He, Peibo Yuan, Zhigang She, Yongjun Lu Source Type: research
Social determinants of therapy failure and multi drug resistance among people with tuberculosis: A review
Social determinants influence health and the development of tuberculosis (TB). However, a paucity of data is available considering the relationship of social determinants influencing therapy failure and multi drug resistance (MDR). We conducted a review investigating the relationship of common social determinants on therapy failure and MDR in people with TB. (Source: Tuberculosis)
Source: Tuberculosis - January 12, 2017 Category: Respiratory Medicine Authors: Francesco Di Gennaro, Damiano Pizzol, Bonifacio Cebola, Brendon Stubbs, Laura Monno, Annalisa Saracino, Claudio Luchini, Marco Solmi, Giulia Segafredo, Giovanni Putoto, Nicola Veronese Tags: Review Source Type: research
Single nucleotide polymorphisms may explain the contrasting phenotypes of two variants of a multidrug-resistant Mycobacterium tuberculosis strain
In this study, we sequenced the whole genomes of a highly prosperous M-family strain (Mp) and its contemporary variant, strain 410, which produced only one recorded tuberculosis case in the last two decades. (Source: Tuberculosis)
Source: Tuberculosis - January 3, 2017 Category: Respiratory Medicine Authors: Mar ía Mercedes Bigi, Beatriz Lopez, Federico Carlos Blanco, María del Carmen Sasiain, Silvia De la Barrera, Marcelo A. Marti, Ezequiel Jorge Sosa, Darío Augusto Fernández Do Porto, Viviana Ritacco, Fabiana Bigi, Marcelo Abel Soria Source Type: research
A deletion hampering appropriate typing of Mycobacterium africanum
Molecular epidemiology analysis of tuberculosis transmission is based mostly on the application of MIRU-VNTR. In certain isolates a complete 24-loci genotype is not obtained and these incompletely genotyped isolates can not be used in the definition of clusters. In a population-based molecular epidemiology study performed in Almer ía, Southeast Spain, a context with a high proportion of immigrants, we found that an 88-bp deletion in isolates of Mycobacterium africanum Lineage 5 hampers MIRU-VNTR analysis. (Source: Tuberculosis)
Source: Tuberculosis - December 25, 2016 Category: Respiratory Medicine Authors: Estefan ía Abascal, Diana Maricela Herrera, Marta Herranz, Sheila Santantón, Miguel Martínez Lirola, Griselda Tudó, Juliá Gonzalez, Emilio Bouza, Laura Pérez-Lago, Darío García-de-Viedma Source Type: research
Local targeting NF- κB in the lung tissue of TB-infected mice diminishes the level of pathology
Mice of the genetically TB-susceptible strain I/St were infected with ∼100 CFU of Mycobacterium tuberculosis strain H37Rv, and after week 3 post-infection treated by inhalations of the NBD peptide selectively blocking NF-κB activation pathway. This therapy resulted in a pronounced attenuation of lung pathology and down-regulation of the expression of several genes encoding major inflammatory molecules, but did not diminish the level of mycobacterial multiplication in the lungs. (Source: Tuberculosis)
Source: Tuberculosis - December 21, 2016 Category: Respiratory Medicine Authors: Galena Shepelkova, Vladimir Evstifeev, Tatiana Kondratieva, Irina Bocharova, Mikhail Averbakh, Alexander Apt Source Type: research
C5aR contributes to the weak Th1 profile induced by an outbreak strain of Mycobacterium tuberculosis
C5a anaphylatoxin is a component of the complement system involved in the modulation of T-cell polarization. Herein we investigated whether C5a receptors, C5aR and C5L2, modulate the cytokine profiles induced by Mycobacterium tuberculosis (Mtb). We analyzed the impact of both receptors on T helper cell polarization induced by the multidrug resistant outbreak strain named M, which is a poor IFN- γ inducer compared with the laboratory strain H37Rv. To this aim, we first blocked C5aR or C5L2 of peripheral blood monocytes (Mo) from patients with tuberculosis and healthy donors, then we stimulated the Mo either with H37Rv or t...
Source: Tuberculosis - December 20, 2016 Category: Respiratory Medicine Authors: Carmen Alejandra Sabio y Garc ía, Noemí Yokobori, Juan Ignacio Basile, Luciana Balboa, Alejandra González, Beatriz López, Viviana Ritacco, Silvia de la Barrera, María del Carmen Sasiain Source Type: research
Role and contribution of pulmonary CD103+ dendritic cells in the adaptive immune response to Mycobacterium tuberculosis
Despite international control programmes, the global burden of tuberculosis remains enormous. Efforts to discover novel drugs have largely focused on targeting the bacterium directly. Alternatively, manipulating the host immune response may represent a valuable approach to enhance immunological clearance of the bacilli, but necessitates a deeper understanding of the immune mechanisms associated with protection against Mycobacterium tuberculosis infection. Here, we examined the various dendritic cells (DC) subsets present in the lung and draining lymph nodes (LN) from mice intra-tracheally infected with M. (Source: Tuberculosis)
Source: Tuberculosis - December 7, 2016 Category: Respiratory Medicine Authors: Vanessa Hui Qi Koh, See Liang Ng, Michelle Lay Teng Ang, Lin Wenwei, Christiane Ruedl, Sylvie Alonso Source Type: research
Anti-mycobacterial function of macrophages is impaired in a diet induced model of type 2 diabetes
In this study, a diet induced murine model of T2D (DIMT2D) was developed and characterized in the context of metabolic, biochemical and histopathological features following diet intervention. Mycobacterial susceptibility was investigated using Mycobacterium fortuitum as a surrogate. Phagocytic capability of alveolar macrophages and resident peritoneal macrophages were determined by in vitro assays using mycolic acid coated beads and M. (Source: Tuberculosis)
Source: Tuberculosis - December 6, 2016 Category: Respiratory Medicine Authors: Md Abdul Alim, Suchandan Sikder, Tahnee L. Bridson, Catherine M. Rush, Brenda L. Govan, Natkunam Ketheesan Source Type: research
Advances in basic and translational tuberculosis research
RePORT International is a collaborative research network of investigators from multiple countries and institutions with the goal of establishing a bio-repository of specimens and clinical data for the study of active TB and latent TB infection (LTBI). During the first meeting of RePORT International in Boston, Massachusetts, the results of research pertinent to TB control and eradication were presented, including advances in the research of Mycobacterium tuberculosis (MTB) persistence and drug resistance, TB diagnostics, drug and vaccine development. (Source: Tuberculosis)
Source: Tuberculosis - November 25, 2016 Category: Respiratory Medicine Authors: Carolina Geadas, Sonia K. Stoszek, David Sherman, Bruno B. Andrade, Sudha Srinivasan, Carol D. Hamilton, Jerrold Ellner Tags: Review Source Type: research
Advances in basic and translational tuberculosis research proceedings of the first meeting of RePORT international
RePORT International is a collaborative research network of investigators from multiple countries and institutions with the goal of establishing a bio-repository of specimens and clinical data for the study of active TB and latent TB infection (LTBI). During the first meeting of RePORT International in Boston, Massachusetts, the results of research pertinent to TB control and eradication were presented, including advances in the research of Mycobacterium tuberculosis (MTB) persistence and drug resistance, TB diagnostics, drug and vaccine development. (Source: Tuberculosis)
Source: Tuberculosis - November 25, 2016 Category: Respiratory Medicine Authors: Carolina Geadas, Sonia K. Stoszek, David Sherman, Bruno B. Andrade, Sudha Srinivasan, Carol D. Hamilton, Jerrold Ellner Tags: Review Source Type: research
B-lymphocytes forming follicle-like structures in the lung tissue of tuberculosis-infected mice: Dynamics, phenotypes and functional activity
During tuberculosis (TB) infection, B cells form follicles in close vicinity of lung granuloma. We assessed the dynamics of follicle formation, surface phenotypes and functional activity of lung B cells during TB course in genetically susceptible mice. The follicles appeared early post infection and peaked at weeks 7 –8. Lung B cells resembled classical B2 cells (CD19+IgMloIgDhiCD1d−CD21/35intCD5−CD11b−CD43−), but differed from them by the absence of B2 marker CD23. Lung B-cells constitutively expressed MHC II molecules, presented mycobacterial antigens to immune CD4+ T-cells and produced high amounts of IL-6 and...
Source: Tuberculosis - November 16, 2016 Category: Respiratory Medicine Authors: Irina Linge, Alexander Dyatlov, Elena Kondratieva, Vadim Avdeenko, Alexander Apt, Tatiana Kondratieva Source Type: research
Obituary Pra. Caterina Mammina 1957 –2016
Professora Caterina Mammina passed away on 2016 September 24th at the age of 60 years after a year of suffering and treatment. Caterina was an epidemiologist. Living in Monreale on the hills of Palermo, her father was a general practitioner, she had graduated in medicine in 1982 and specialized in hygiene and preventive medicine at the University of Palermo (Unipa) in 1985; She had followed many post-graduate courses at the Scuola Internazionale di Epidemiologia e Medicina Preventiva “Giuseppe D'Alessandro”, Centro “Ettore Majorana”, Erice (Trapani) in 1984, 1990, 2000, and 2002. (Source: Tuberculosis)
Source: Tuberculosis - November 14, 2016 Category: Respiratory Medicine Authors: Celestino Bonura, Christophe Sola, Francesco Vitale Source Type: research
Association between SLC11A1 (NRAMP1) polymorphisms and susceptibility to tuberculosis in Chinese Holstein cattle
We investigated the associations between SLC11A1 (NRAMP1) polymorphisms and susceptibility to tuberculosis (TB) in Chinese Holstein cattle from the Yunnan Plateau, China, using a case-control study. The case group included 136 animals that had positive reactions to both a purified protein derivative test and an IFN- γ assay, as well as showing clinical symptoms of TB, and the control group included 96 animals that had negative reactions to both testsand showed no symptoms. Polymerase chain reaction (PCR) sequencing and the PCR-restriction fragment length polymorphism (RFLP) technique were used to detect and de termineSLC1...
Source: Tuberculosis - November 7, 2016 Category: Respiratory Medicine Authors: Kaihua Liu, Bin Zhang, Zhaochun Teng, Youtao Wang, Guodong Dong, Cong Xu, Bo Qin, Chunlian Song, Jun Chai, Yang Li, Xianwei Shi, Xianghua Shu, Yifang Zhang Source Type: research
Mycobacterium tuberculosis Rv0560c is not essential for growth in vitro or in macrophages
Mycobacterium tuberculosis Rv0560c, a putative benzoquinone methyl transferase, is heavily induced in response to salicylate exposure. It has some similarity to Escherichia coli UbiG, although its role in ubiquinone or menaquinone synthesis is not clear, since M. tuberculosis is not known to produce ubiquinone. We constructed an unmarked in-frame deletion of Rv0560c in M. tuberculosis to determine its role in vitro. Deletion of Rv0560c in M. tuberculosis had no effect on growth in medium containing salicylate or in its ability to grow in macrophages. (Source: Tuberculosis)
Source: Tuberculosis - November 3, 2016 Category: Respiratory Medicine Authors: Rachel Kokoczka, Doroth ée L. Schuessler, Julie V. Early, Tanya Parish Source Type: research
Mycobacterium tuberculosis Rv0560c is not essential for growth in vitro or in macrophages
Mycobacterium tuberculosis Rv0560c, a putative benzoquinone methyl transferase, is heavily induced in response to salicylate exposure. It has some similarity to Escherichia coli UbiG, although its role in ubiquinone or menaquinone synthesis is not clear, since M. tuberculosis is not known to produce ubiquinone. We constructed an unmarked in-frame deletion of Rv0560c in M. tuberculosis to determine its role in vitro. Deletion of Rv0560c in M. tuberculosis had no effect on growth in medium containing salicylate or in its ability to grow in macrophages. (Source: Tuberculosis)
Source: Tuberculosis - November 3, 2016 Category: Respiratory Medicine Authors: Rachel Kokoczka, Doroth ée L. Schuessler, Julie V. Early, Tanya Parish Source Type: research
