Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil
Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position. Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin (RIF) resistance.In the present study, we characterized a total of 63 drug r...
Source: Tuberculosis - March 1, 2018 Category: Respiratory Medicine Authors: Leonardo Souza Esteves, Elis Regina Dalla Costa, Sidra Ezidio Gon çalves Vasconcellos, Andrei Vargas, Sérgio Luis Montego Ferreira Junior, Maria Laura Halon, Marta Osorio Ribeiro, Rodrigo Rodenbusch, Harrison Magdinier Gomes, Philip N. Suffys, Maria Luc Source Type: research
Novel red fluorescence protein based microplate assay for drug screening against dormant Mycobacterium tuberculosis by using paraffin
The hypoxia model of dormancy is widely used in drug screening programs to identify novel inhibitors against latent Mycobacterium tuberculosis disease. In earlier reported microplate assays, hypoxia was maintained by either sealing the microplate or shifting in an anaerobic chamber to develop dormant phenotype. In these assays, inhibitors were added during inoculation, which mainly represents the active stage inhibitors instead of the dormant ones. Herein, the culture was covered with paraffin to develop hypoxia condition and consequently providing the advantage of adding compounds at any stage during incubation of 96-well...
Source: Tuberculosis - February 26, 2018 Category: Respiratory Medicine Authors: Amar Yeware, Dhiman Sarkar Source Type: research
Title: Novel red fluorescence protein based microplate assay for drug screening against dormant Mycobacterium tuberculosis by using paraffin
The hypoxia model of dormancy is widely used in drug screening programs to identify novel inhibitors against latent Mycobacterium tuberculosis disease. In earlier reported microplate assays, hypoxia was maintained by either sealing the microplate or shifting in an anaerobic chamber to develop dormant phenotype. In these assays, inhibitors were added during inoculation, which mainly represents the active stage inhibitors instead of the dormant ones. Herein, the culture was covered with paraffin to develop hypoxia condition and consequently providing the advantage of adding compounds at any stage during incubation of 96-well...
Source: Tuberculosis - February 26, 2018 Category: Respiratory Medicine Authors: Amar Yeware, Dhiman Sarkar Source Type: research
Experimental animal models of central nervous system tuberculosis: A historical review
Animal models are and will remain valuable tools in medical research because their use enables a deeper understanding of disease development, thus generating important knowledge for developing disease control strategies. Central nervous system tuberculosis (CNS TB) is the most devastating disease in humans. Moreover, as the variability of signs and symptoms delay a timely diagnosis, patients usually arrive at the hospital suffering from late stage disease. Therefore, it is impossible to obtain fresh human tissue for research before an autopsy. (Source: Tuberculosis)
Source: Tuberculosis - February 25, 2018 Category: Respiratory Medicine Authors: Carlos S ánchez-Garibay, María Elena Hernández-Campos, Martha Lilia Tena-Suck, Citlaltepetl Salinas-Lara Tags: Review Source Type: research
Genotypic drug resistance using whole-genome sequencing of Mycobacterium tuberculosis clinical isolates from North-western Tanzania
Drug resistant Tuberculosis (TB) is considered a global public health threat. Whole-genome sequencing (WGS) is a new technology for tuberculosis (TB) diagnostics and is capable of providing rapid drug resistance profiles and genotypes for epidemiologic surveillance. Therefore, we used WGS to determine genotypic drug resistance profiles and genetic diversity of drug resistant Mycobacterium tuberculosis isolates from Mwanza, North-western Tanzania. (Source: Tuberculosis)
Source: Tuberculosis - February 21, 2018 Category: Respiratory Medicine Authors: Benson R. Kidenya, Stephen E. Mshana, Daniel W. Fitzgerald, Oksana Ocheretina Source Type: research
Meta-analysis of the lung microbiota in pulmonary tuberculosis
The lung microbiota has received less attention compared to other body sites, in part because its study carries special technological difficulties related to obtaining reliable samples as compared to other body niches. The limited number of studies on the sputum microbiota on TB patients and controls available so far have reported inconsistent, and sometimes, contradictory results. Aiming to clarify if changes in the lung microbiota composition are associated with pulmonary TB, we performed a meta-analysis of available data on microbiota of the lower respiratory tract in TB patients and healthy controls. (Source: Tuberculosis)
Source: Tuberculosis - February 21, 2018 Category: Respiratory Medicine Authors: Bo-young Hong, Joseph N. Paulson, O. Colin Stine, George M. Weinstock, Jorge L. Cervantes Source Type: research
Genotypic drug resistance using whole-genome sequencing of Mycobacterium tuberculosis clinical isolates from North-western Tanzania
Drug resistant Tuberculosis (TB) is considered a global public health threat. Whole-genome sequencing (WGS) is a new technology for tuberculosis (TB) diagnostics and is capable of providing rapid drug resistance profiles and genotypes for epidemiologic surveillance. Therefore, we used WGS to determine genotypic drug resistance profiles and genetic diversity of drug resistant Mycobacterium tuberculosis isolates from Mwanza, North-western Tanzania. (Source: Tuberculosis)
Source: Tuberculosis - February 21, 2018 Category: Respiratory Medicine Authors: Benson R. Kidenya, Stephen E. Mshana, Daniel W. Fitzgerald, Oksana Ocheretina Source Type: research
Meta-analysis of the lung microbiota in pulmonary tuberculosis
The lung microbiota has received less attention compared to other body sites, in part because its study carries special technological difficulties related to obtaining reliable samples as compared to other body niches. The limited number of studies on the sputum microbiota on TB patients and controls available so far have reported inconsistent, and sometimes, contradictory results. Aiming to clarify if changes in the lung microbiota composition are associated with pulmonary TB, we performed a meta-analysis of available data on microbiota of the lower respiratory tract in TB patients and healthy controls. (Source: Tuberculosis)
Source: Tuberculosis - February 21, 2018 Category: Respiratory Medicine Authors: Bo-young Hong, Joseph N. Paulson, O. Colin Stine, George M. Weinstock, Jorge L. Cervantes Source Type: research
Immunoendocrine abnormalities in the male reproductive system during experimental pulmonary tuberculosis
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) that mainly affects the lungs. Along the course of pulmonary TB there are remarkable changes in the production of cytokines that cause endocrine changes. So far, it is not known the physiological and histological changes in the male reproductive system during pulmonary TB. (Source: Tuberculosis)
Source: Tuberculosis - February 20, 2018 Category: Respiratory Medicine Authors: Brenda Ramos Robles, Ricardo A. Valdez, Uriel Ju árez Hernández, Brenda Marquina Castillo, Dulce Mata Espinosa, Jorge Barrios Payan, Rogelio Hernández Pando, Marta C. Romano Source Type: research
Biophysical and immunological characterization of the ESX-4 system ESAT-6 family proteins Rv3444c and Rv3445c from Mycobacterium tuberculosis H37Rv
The ESAT-6 family proteins of Mycobacterium tuberculosis are regarded as the key mediators in mycobacterial virulence and are largely considered as antigens that can improve TB vaccines and diagnostics. We have characterized Rv3444c and Rv3445c proteins of the ESX-4 system of ESAT-6 family of M. tuberculosis H37Rv, and have experimentally established that these two proteins interact to form a heterodimeric complex. Complex formation resulted in induction of α-helical conformation and stability against chemical denaturation. (Source: Tuberculosis)
Source: Tuberculosis - February 12, 2018 Category: Respiratory Medicine Authors: Himanshu Pandey, Farheen Fatma, Shivraj M. Yabaji, Meera Kumari, Sarita Tripathi, Kanchan Srivastava, Dinesh K. Tripathi, Surya Kant, Kishore K. Srivastava, Ashish Arora Tags: Immunological Aspects Source Type: research
Biology of clinical strains of Mycobacterium tuberculosis with varying levels of transmission
In this study we established a panel of clinical isolates of M. tuberculosis strains obtained from the Western Cape region of South Africa, each of which had been carefully tracked in terms of their degree of transmission in the community. (Source: Tuberculosis)
Source: Tuberculosis - February 9, 2018 Category: Respiratory Medicine Authors: Crystal A. Shanley, Marcela I. Henao-Tamayo, Chand Bipin, Raja Mugasimangalam, Deepshika Verma, Diane J. Ordway, Elizabeth M. Streicher, Ian M. Orme Tags: Mechanisms of Pathogenesis Source Type: research
