Biophysical and immunological characterization of the ESX-4 system ESAT-6 family proteins Rv3444c and Rv3445c from Mycobacterium tuberculosis H37Rv

The ESAT-6 family proteins of Mycobacterium tuberculosis are regarded as the key mediators in mycobacterial virulence and are largely considered as antigens that can improve TB vaccines and diagnostics. We have characterized Rv3444c and Rv3445c proteins of the ESX-4 system of ESAT-6 family of M. tuberculosis H37Rv, and have experimentally established that these two proteins interact to form a heterodimeric complex. Complex formation resulted in induction of α-helical conformation and stability against chemical denaturation.

Source: Tuberculosis - February 12, 2018 Category: Respiratory Medicine Authors: Himanshu Pandey, Farheen Fatma, Shivraj M. Yabaji, Meera Kumari, Sarita Tripathi, Kanchan Srivastava, Dinesh K. Tripathi, Surya Kant, Kishore K. Srivastava, Ashish Arora Tags: Immunological Aspects Source Type: research