On sunspots, click science and molecular iconography
CRISPR-spoligotyping and MIRU-VNTR typing, SITVIT_WEB and MIRU-VNTRplus are the methods and online resources most widely used for Mycobacterium tuberculosis genotype family assignment and clustering analysis. They have been proven invaluable for molecular epidemiological studies of this important human pathogen in setting up the terminology and classification framework. However, they are inherently limited by insufficient knowledge of evolution of the targeted genome loci (especially, CRISPR). The situation is aggravated by the dogmatic, iconographic perception of these increasingly user-friendly online tools. (Source: Tuberculosis)
Source: Tuberculosis - April 10, 2018 Category: Respiratory Medicine Authors: Igor Mokrousov Tags: Opinion Source Type: research
Identification of TB-E12 as a novel FtsZ inhibitor with anti-tuberculosis activity
In this study, we used phenotype screening of M. smegmatis and model screening targeting M. tuberculosis FtsZ (Mtb-FtsZ) to identify a hit compound TB-E12. (Source: Tuberculosis)
Source: Tuberculosis - April 5, 2018 Category: Respiratory Medicine Authors: Yuan Lin, Hongjuan Zhang, Ningyu Zhu, Xia Wang, Yanxing Han, Minghua Chen, Jiandong Jiang, Shuyi Si Source Type: research
Accuracy of whole genome sequencing versus phenotypic (MGIT) and commercial molecular tests for detection of drug-resistant Mycobacterium tuberculosis isolated from patients in Brazil and Mozambique
The fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST. (Source: Tuberculosis)
Source: Tuberculosis - April 5, 2018 Category: Respiratory Medicine Authors: Cinara Silva Feliciano, Evangelina Inacio Namburete, J éssica Rodrigues Plaça, Kamila Peronni, Anzaan Dippenaar, Robin Mark Warren, Wilson Araújo Silva, Valdes Roberto Bollela Source Type: research
Identification of TB-E12 as a novel FtsZ inhibitor with anti-tuberculosis activity
In this study, we used phenotype screening of M. smegmatis and model screening targeting M. tuberculosis FtsZ (Mtb-FtsZ) to identify a hit compound TB-E12. (Source: Tuberculosis)
Source: Tuberculosis - April 5, 2018 Category: Respiratory Medicine Authors: Yuan Lin, Hongjuan Zhang, Ningyu Zhu, Xia Wang, Yanxing Han, Minghua Chen, Jiandong Jiang, Shuyi Si Source Type: research
Accuracy of whole genome sequencing versus phenotypic (MGIT) and commercial molecular tests for detection of drug-resistant Mycobacterium tuberculosis isolated from patients in Brazil and Mozambique
The fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST. (Source: Tuberculosis)
Source: Tuberculosis - April 5, 2018 Category: Respiratory Medicine Authors: Cinara Silva Feliciano, Evangelina Namburete, J éssica Rodrigues Plaça, Kamila Peronni, Anzaan Dippenaar, Robin Mark Warren, Wilson Araújo Silva, Valdes Roberto Bollela Source Type: research
The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. (Source: Tuberculosis)
Source: Tuberculosis - April 4, 2018 Category: Respiratory Medicine Authors: Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E. Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H. Gillespie, Michael Hoelscher, PanACEA-Consortium Source Type: research
RDRio Mycobacterium tuberculosis lineage in the Brazil/Paraguay/Argentina triple border
The high tuberculosis (TB) incidence rates, the closeness of the cities and the high migration flux on the Brazil/Paraguay/Argentina border deserves an in-depth study, using Mycobacterial Interspersed Repetitive Unit (MIRU) and Spoligotyping genetic markers to explore the impact of the Mycobacterium tuberculosis RDRio lineage on disease transmission and resistance to anti-TB drugs in this setting. Although without the totality of M. tuberculosis isolates causing TB in this studied setting, a number of 97 isolates obtained from sputa samples culture of patients with confirmed TB, from 2013 to 2015, were submitted to 24 loci...
Source: Tuberculosis - March 31, 2018 Category: Respiratory Medicine Authors: Aryadne Larissa de Almeida, Regiane Bertin de Lima Scodro, Hayalla Corr êa de Carvalho, Giovana Ferreira Costacurta, Vanessa Pietrowski Baldin, Nathally Claudiane Souza Santos, Luciana Dias Ghiraldi-Lopes, Paula Aline Zanetti Campanerut-Sá, Vera Lucia D Source Type: research
Corrigendum to “Mechanisms of resistance to delamanid, a drug for Mycobacterium tuberculosis” [Tuberculosis 108 (January 2018) 186–194]
The authors regret that there is a mix-up of the footnote on Table 4 of our published paper in Tuberculosis. (Source: Tuberculosis)
Source: Tuberculosis - March 31, 2018 Category: Respiratory Medicine Authors: Mamoru Fujiwara, Masanori Kawasaki, Norimitsu Hariguchi, Yongge Liu, Makoto Matsumoto Tags: Corrigendum Source Type: research
RDRio Mycobacterium tuberculosis lineage in the Brazil/Paraguay/Argentina triple border
The high tuberculosis (TB) incidence rates, the closeness of the cities and the high migration flux on the Brazil/Paraguay/Argentina border deserves an in-depth study, using Mycobacterial Interspersed Repetitive Unit (MIRU) and Spoligotyping genetic markers to explore the impact of the Mycobacterium tuberculosis RDRio lineage on disease transmission and resistance to anti-TB drugs in this setting. Although without the totality of M. tuberculosis isolates causing TB in this studied setting, a number of 97 isolates obtained from sputa samples culture of patients with confirmed TB, from 2013 to 2015, were submitted to 24 loci...
Source: Tuberculosis - March 31, 2018 Category: Respiratory Medicine Authors: Aryadne Larissa de Almeida, Regiane Bertin de Lima Scodro, Hayalla Corr êa de Carvalho, Giovana Ferreira Costacurta, Vanessa Pietrowski Baldin, Nathally Claudiane Souza Santos, Luciana Dias Ghiraldi-Lopes, Paula Aline Zanetti Campanerut-Sá, Vera Lucia D Source Type: research
Corrigendum to “Mechanisms of resistance to delamanid, a drug for Mycobacterium tuberculosis” [Tuberculosis 108 (January 2018) 186–194]
The authors regret that there is a mix-up of the footnote on Table 4 of our published paper in Tuberculosis. (Source: Tuberculosis)
Source: Tuberculosis - March 31, 2018 Category: Respiratory Medicine Authors: Mamoru Fujiwara, Masanori Kawasaki, Norimitsu Hariguchi, Yongge Liu, Makoto Matsumoto Tags: Corrigendum Source Type: research
Tag Array gene chip rapid diagnosis anti-tuberculosis drug resistance in pulmonary tuberculosis -a feasibility study
We developed a Tag Array chip for detecting first- and second-line anti tuberculosis drug resistance in pulmonary tuberculosis and compared the analytical performance of the gene chip to that of phenotypic drug susceptibility testing (DST). (Source: Tuberculosis)
Source: Tuberculosis - March 27, 2018 Category: Respiratory Medicine Authors: Wenjie Wu, Peng Cheng, Jingtong Lyu, Zehua Zhang, jianzhong Xu Tags: Diagnostics Source Type: research
Dissecting whole-genome sequencing-based online tools for predicting resistance in Mycobacterium tuberculosis: can we use them for clinical decision guidance?
Whole-genome sequencing (WGS)-based bioinformatics platforms for the rapid prediction of resistance will soon be implemented in the Tuberculosis (TB) laboratory, but their accuracy assessment still needs to be strengthened. Here, we fully-sequenced a total of 54 multidrug-resistant (MDR) and five susceptible TB strains and performed, for the first time, a simultaneous evaluation of the major four free online platforms (TB Profiler, PhyResSE, Mykrobe Predictor and TGS-TB).Overall, the sensitivity of resistance prediction ranged from 84.3% using Mykrobe predictor to 95.2% using TB profiler, while specificity was higher and h...
Source: Tuberculosis - March 27, 2018 Category: Respiratory Medicine Authors: Rita Macedo, Alexandra Nunes, Isabel Portugal, S ílvia Duarte, Luís Vieira, João Paulo Gomes Source Type: research
Tag Array gene chip rapid diagnosis anti-tuberculosis drug resistance in pulmonary tuberculosis -a feasibility study
We developed a Tag Array chip for detecting first- and second-line anti tuberculosis drug resistance in pulmonary tuberculosis and compared the analytical performance of the gene chip to that of phenotypic drug susceptibility testing (DST). (Source: Tuberculosis)
Source: Tuberculosis - March 27, 2018 Category: Respiratory Medicine Authors: Wenjie Wu, Peng Cheng, Jingtong Lyu, Zehua Zhang, jianzhong Xu Tags: Diagnostics Source Type: research
