Dissecting whole-genome sequencing-based online tools for predicting resistance in Mycobacterium tuberculosis: can we use them for clinical decision guidance?
Whole-genome sequencing (WGS)-based bioinformatics platforms for the rapid prediction of resistance will soon be implemented in the Tuberculosis (TB) laboratory, but their accuracy assessment still needs to be strengthened. Here, we fully-sequenced a total of 54 multidrug-resistant (MDR) and five susceptible TB strains and performed, for the first time, a simultaneous evaluation of the major four free online platforms (TB Profiler, PhyResSE, Mykrobe Predictor and TGS-TB).Overall, the sensitivity of resistance prediction ranged from 84.3% using Mykrobe predictor to 95.2% using TB profiler, while specificity was higher and h...
Source: Tuberculosis - March 27, 2018 Category: Respiratory Medicine Authors: Rita Macedo, Alexandra Nunes, Isabel Portugal, S ílvia Duarte, Luís Vieira, João Paulo Gomes Source Type: research
Evaluation of pyrosequencing for extensive drug resistance-defining anti-tuberculosis drugs for use in public healthcare
MGIT 960 drug susceptibility testing (DST) for Mycobacterium tuberculosis was compared for performance and speed with pyrosequencing (PSQ). Pulmonary samples (n = 100), from GeneXpert/MTB/Rifampicin-resistant patients receiving second-line treatment for 1–3 months, were subjected to DST and PSQ for seven drugs (isoniazid, rifampicin, kanamycin, amikacin, capreomycin, moxifloxacin, and ofloxacin). The mean time-to-result was 35 and two days for DST an d PSQ, respectively. Average concordancy was 92.7%. (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Remya Nambiar, Daksha Shah, Kanchan Ajbani, Mubin Kazi, Meeta Sadani, Anjali Shetty, Padmaja Keskar, Sanjeev Kamble, Alex van Belkum, Camilla Rodrigues Source Type: research
Reverse line probe assay for cheap detection of Single Nucleotide Polymorphisms in Mycobacterium tuberculosis
This study presents a low-cost reverse hybridization line probe technique for detecting SNPs in Mycobacterium tuberculosis. The proposed test is able to detect mutations in the RRDR of rpoB gene in M. tuberculosis with specificity and sensitivity of 98% and 100%, respectively and for an average cost of less than €3 per sample. (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Memona Yasmin, Guislaine Refregier, Rubina Tabassum Siddiqui, Rizwan Iqbal, Shahid Ahmad Abbasi, Sabira Tahseen Tags: Diagnostics Source Type: research
Normalised quantitative polymerase chain reaction for diagnosis of tuberculosis-associated uveitis
In this study, we investigated normalised quantitative PCR (nqPCR) in ocular fluids (aqueous/vitreous) for diagnosis of TBU in a TB-endemic population. Mycobacterial copy numbers (mpb64 gene) were normalised to host genome copy numbers (RNAse P RNA component H1 [RPPH1] gene) in TBU (n = 16) and control (n = 13) samples (discovery cohort). (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Manas Ranjan Barik, Soveeta Rath, Rohit Modi, Rajkishori Rana, Mamatha M. Reddy, Soumyava Basu Source Type: research
Evaluation of pyrosequencing for extensive drug resistance-defining anti-tuberculosis drugs for use in public healthcare
MGIT 960 drug susceptibility testing (DST) for Mycobacterium tuberculosis was compared for performance and speed with pyrosequencing (PSQ). Pulmonary samples (n = 100), from GeneXpert/MTB/Rifampicin-resistant patients receiving second-line treatment for 1–3 months, were subjected to DST and PSQ for seven drugs (isoniazid, rifampicin, kanamycin, amikacin, capreomycin, moxifloxacin, and ofloxacin). The mean time-to-result was 35 and two days for DST an d PSQ, respectively. Average concordancy was 92.7%. (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Remya Nambiar, Daksha Shah, Kanchan Ajbani, Mubin Kazi, Meeta Sadani, Anjali Shetty, Padmaja Keskar, Sanjeev Kamble, Alex van Belkum, Camilla Rodrigues Source Type: research
Reverse line probe assay for cheap detection of Single Nucleotide Polymorphisms in Mycobacterium tuberculosis
This study presents a low-cost reverse hybridization line probe technique for detecting SNPs in Mycobacterium tuberculosis. The proposed test is able to detect mutations in the RRDR of rpoB gene in M. tuberculosis with specificity and sensitivity of 98% and 100%, respectively and for an average cost of less than €3 per sample. (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Memona Yasmin, Guislaine Refregier, Rubina Tabassum Siddiqui, Rizwan Iqbal, Shahid Ahmed Abbasi, Sabira Tahseen Tags: Diagnostics Source Type: research
Normalised quantitative polymerase chain reaction for diagnosis of tuberculosis-associated uveitis
In this study, we investigated normalised quantitative PCR (nqPCR) in ocular fluids (aqueous/vitreous) for diagnosis of TBU in a TB-endemic population. Mycobacterial copy numbers (mpb64 gene) were normalised to host genome copy numbers (RNAse P RNA component H1 [RPPH1] gene) in TBU (n = 16) and control (n = 13) samples (discovery cohort). (Source: Tuberculosis)
Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Manas Ranjan Barik, Soveeta Rath, Rohit Modi, Rajkishori Rana, Mamatha M. Reddy, Soumyava Basu Source Type: research
Genetic diversity of antigen 38 kDa in Mycobacterium tuberculosis strains from China
We used 335 Mycobacterium tuberculosis strains from 2010 National Epidemiologic Survey for TB in China and performed comparative sequence analysis of 38 kDa gene after amplification. From the results, we found that there were 5.07% M.tuberculosis strains that demonstrated genetic diversity of 38 kDa in China, and 2.99% strains showed polymorphism of the 38 kDa antigen, and this may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion. (Source: Tuberculosis)
Source: Tuberculosis - March 14, 2018 Category: Respiratory Medicine Authors: Yingzhen Du, Hongxia Li, Xuebing Qin, Yan Wang, Zhijian Zhang, Geping Qu, Xiangqun Fang, Senyang Yu Source Type: research
Seasonal variation in the performance of QuantiFERON-TB Gold In-Tube assays used for the diagnosis of tuberculosis infection
This study aimed to determine whether there are seasonal changes in the performance of QuantiFERON-TB Gold In-Tube (QFT-GIT) assays, an interferon-gamma release assay widely used for the diagnosis of tuberculosis infection. Results of 31,932 QFT-GIT assays performed at a large independent, accredited diagnostic service provider in London, UK over a 4.5-year-period were analysed. The proportion of positive results was significantly lower in autumn (14.8%) than in spring (16.0%; p = 0.0366) and summer (17.5%; p (Source: Tuberculosis)
Source: Tuberculosis - March 8, 2018 Category: Respiratory Medicine Authors: Marc Tebruegge, Nigel Curtis, Vanessa Clifford, Cristina Fernandez-Turienzo, Nigel Klein, Katy Fidler, Salah Mansour, Paul Elkington, Stephen Morris-Jones Source Type: research
Interleukin 23/interleukin 17 axis activated by Mycobacterium avium complex (MAC) is attenuated in patients with MAC-lung disease
Mycobacterium avium complex (MAC)-lung disease (LD) is increasing in patients without human immunodeficiency virus infection. However, data on host vulnerability to MAC-related immune responses, and in particular the interleukin (IL)-23/IL-17 axis, are lacking. (Source: Tuberculosis)
Source: Tuberculosis - March 3, 2018 Category: Respiratory Medicine Authors: Chin-Chung Shu, Jann-Yuan Wang, Ming-Fang Wu, Hsin-Chih Lai, Bor-Luen Chiang, Chong-Jen Yu Tags: Non-Tuberculous Mycobacteria: General Source Type: research
Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil
Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position. Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin (RIF) resistance.In the present study, we characterized a total of 63 drug r...
Source: Tuberculosis - March 1, 2018 Category: Respiratory Medicine Authors: Leonardo Souza Esteves, Elis Regina Dalla Costa, Sidra Ezidio Gon çalves Vasconcellos, Andrei Vargas, Sérgio Luis Montego Ferreira Junior, Maria Laura Halon, Marta Osorio Ribeiro, Rodrigo Rodenbusch, Harrison Magdinier Gomes, Philip N. Suffys, Maria Luc Source Type: research
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(Source: Tuberculosis)
Source: Tuberculosis - March 1, 2018 Category: Respiratory Medicine Source Type: research
