Evaluation of pyrosequencing for extensive drug resistance-defining anti-tuberculosis drugs for use in public healthcare

MGIT 960 drug susceptibility testing (DST) for Mycobacterium tuberculosis was compared for performance and speed with pyrosequencing (PSQ). Pulmonary samples (n  = 100), from GeneXpert/MTB/Rifampicin-resistant patients receiving second-line treatment for 1–3 months, were subjected to DST and PSQ for seven drugs (isoniazid, rifampicin, kanamycin, amikacin, capreomycin, moxifloxacin, and ofloxacin). The mean time-to-result was 35 and two days for DST an d PSQ, respectively. Average concordancy was 92.7%.

Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Remya Nambiar, Daksha Shah, Kanchan Ajbani, Mubin Kazi, Meeta Sadani, Anjali Shetty, Padmaja Keskar, Sanjeev Kamble, Alex van Belkum, Camilla Rodrigues Source Type: research