Effect of the bradykinin 1 receptor antagonist SSR240612 after oral administration in Mycobacterium tuberculosis-infected mice
The role, if any, played by the kinin system in tuberculosis infection models, either in vivo or in vitro, was investigated. The effects of Mycobacterium tuberculosis infection on C57BL/6 wild type, B1R −/−, B2R−/− and double B1R/B2R knockout mice were evaluated. Immunohistochemistry analysis was carried out to assess B1R and B2R expression in spleens and lungs of M. tuberculosis-infected mice. In addition, in vitro experiments with M. tuberculosis-infected macrophages were performed. The i n vivo effects of HOE-140 and SSR240612 on the mice model of infection were also evaluated. (Source: Tuberculosis)
Source: Tuberculosis - January 19, 2018 Category: Respiratory Medicine Authors: Valn ês S. Rodrigues-Junior, Priscilla B. Pail, Anne D. Villela, Virgínia C.A. Falcão, Adílio S. Dadda, Bruno L. Abbadi, João B. Pesquero, Diógenes S. Santos, Luiz A. Basso, Maria M. Campos Tags: Drug Discovery and Resistance Source Type: research
Existing blood transcriptional classifiers accurately discriminate active tuberculosis from latent infection in individuals from south India
Several studies have identified blood transcriptomic signatures that can distinguish active from latent Tuberculosis (TB). The purpose of this study was to assess how well these existing gene profiles classify TB disease in a South Indian population. RNA sequencing was performed on whole blood PAXgene samples collected from 28 TB patients and 16 latently TB infected (LTBI) subjects enrolled as part of an ongoing household contact study. Differential gene expression and clustering analyses were performed and compared with explicit predictive testing of TB and LTBI individuals based on established gene signatures. (Source: Tuberculosis)
Source: Tuberculosis - January 19, 2018 Category: Respiratory Medicine Authors: Samantha Leong, Yue Zhao, Noyal M. Joseph, Natasha S. Hochberg, Sonali Sarkar, Jane Pleskunas, David Hom, Subitha Lakshminarayanan, C. Robert Horsburgh, Gautam Roy, Jerrold J. Ellner, W. Evan Johnson, Padmini Salgame Tags: Diagnostics Source Type: research
Use of a leukocyte-targeted peptide probe as a potential tracer for imaging the tuberculosis granuloma
We report the binding specificity of Cyanine 3 (Cy3)-labeled cFLFLFK-PEG12 to human leukocytes and cellular constituents within a human in vitro granuloma model. We also report use of the probe in in vivo studies using a mouse model of lung granulomatous inflammation. (Source: Tuberculosis)
Source: Tuberculosis - January 8, 2018 Category: Respiratory Medicine Authors: Landon W. Locke, Shankaran Kothandaraman, Michael Tweedle, Sarah Chaney, Daniel Wozniak, Larry S. Schlesinger Source Type: research
Tuberculosis determination using SERS and chemometric methods
Nanostructures have been multiplying the advantages of Raman spectroscopy and further amplify the advantages of Raman spectroscopy is a continuous effort focused on the appropriate design of nanostructures. Herein, we designed different shapes of plasmonic nanostructures such as Vertical, Zig Zag, Slant nanorods and Spherical nanoparticles employing the DC magnetron sputtering system as SERS-active substrates for ultrasensitive detection of target molecules. The fabricated plasmonic nanostructures sensitivity and uniformity were exploited by reference dye analyte. (Source: Tuberculosis)
Source: Tuberculosis - January 2, 2018 Category: Respiratory Medicine Authors: Raju Botta, Pongpan Chindaudom, Pitak Eiamchai, Mati Horprathum, Saksorn Limwichean, Chanunthorn Chananonnawathorn, Viyapol Patthanasettakul, Benjawan Kaewseekhao, Kiatichai Faksri, Noppadon Nuntawong Tags: Diagnostics Source Type: research
Editorial Board
(Source: Tuberculosis)
Source: Tuberculosis - January 1, 2018 Category: Respiratory Medicine Source Type: research
Mechanisms of resistance to delamanid, a drug for Mycobacterium tuberculosis
Delamanid, a bicyclic nitroimidazooxazole, is effective against M. tuberculosis. Previous studies have shown that resistance to a bicyclic nitroimidazooxazine, PA-824, is caused by mutations in a F420-dependent bio-activation pathway. We investigated whether the same mechanisms are responsible for resistance to delamanid. Spontaneous resistant frequencies were determined using M. bovis BCG Tokyo (BCG) and M. tuberculosis H37Rv. F420 High-performance liquid chromatography (HPLC) elution patterns of homogenates of delamanid resistant BCG colonies and two previously identified delamanid-resistant M. (Source: Tuberculosis)
Source: Tuberculosis - December 29, 2017 Category: Respiratory Medicine Authors: Mamoru Fujiwara, Masanori Kawasaki, Norimitsu Hariguchi, Yongge Liu, Makoto Matsumoto Tags: Drug Discovery and Resistance Source Type: research
Genotypic characterization of pyrazinamide resistance in Mycobacterium tuberculosis isolated from Lusaka, Zambia
Pyrazinamide forms a core part of treatment for all types of tuberculosis (TB) in Zambia. Due to challenges associated with pyrazinamide testing, little information is available to indicate the frequency of resistance to this drug in Zambia. To determine the frequency of pyrazinamide (PZA) resistance and its correlation with mutation in pncA in Mycobacterium tuberculosis isolated from patients in Lusaka, Zambia, BACTEC MGIT M960 was used for phenotypic PZA susceptibility testing while sequencing was used to determine resistance-conferring mutations in the pncA. (Source: Tuberculosis)
Source: Tuberculosis - December 28, 2017 Category: Respiratory Medicine Authors: Precious Bwalya, Tomoyuki Yamaguchi, Georgina Mulundu, Chie Nakajima, Grace Mbulo, Eddie Samuneti Solo, Yukari Fukushima, Kunda Kasakwa, Yasuhiko Suzuki Source Type: research
Utility of the T-SPOT ®.TB test's borderline category to increase test resolution for results around the cut-off point
Accurate identification of individuals with TB infection, is required to achieve the WHO's End TB Strategy goals. While there is general acceptance that the T-SPOT.TB test borderline category provides an opportunity to increase test resolution of results around the test cut-off point, this has not been investigated. (Source: Tuberculosis)
Source: Tuberculosis - December 18, 2017 Category: Respiratory Medicine Authors: Karen Rego, Kristen Pereira, James MacDougall, William Cruikshank Source Type: research
Mycobacterial nucleoid associated proteins: An added dimension in gene regulation
Nucleoid associated proteins (NAPs) are known organisers of chromosomal structure and regulators of transcriptional expression. The number of proposed NAPs in mycobacteria are significantly lower than the number identified in other organisms. An interesting feature of mycobacterial NAPs is their low sequence similarity with those in other species, a property that has hindered their identification. In this review, we discuss the current evidence for the proposed classification of six mycobacterial proteins, Lsr2, EspR, mIHF, HupB, MDP2 and NapM, as NAPs in mycobacterial species with an emphasis on their roles in modulating ...
Source: Tuberculosis - December 13, 2017 Category: Respiratory Medicine Authors: Nastassja L. Kriel, James Gallant, Ni ël van Wyk, Paul van Helden, Samantha L. Sampson, Robin M. Warren, Monique J. Williams Tags: Review Source Type: research
Major genotype families and epidemic clones of Mycobacterium tuberculosis in Omsk region, Western Siberia, Russia, marked by a high burden of tuberculosis-HIV coinfection
This population-based study characterized Mycobacterium tuberculosis isolates from HIV-positive and HIV-negative tuberculosis (TB) patients in the Omsk region in Western Siberia, Russia. We sought to gain insight into the major genotype families and epidemic and endemic clones of M. tuberculosis in the area with a high burden and adverse trend of TB/HIV coinfection. The study collection included M. tuberculosis isolates from 207 newly-diagnosed patients with pulmonary TB; 55 (26.5%) of patients were HIV-infected. (Source: Tuberculosis)
Source: Tuberculosis - December 11, 2017 Category: Respiratory Medicine Authors: Oksana Pasechnik, Anna Vyazovaya, Stanislav Vitriv, Marina Tatarintseva, Aleksey Blokh, Vladimir Stasenko, Igor Mokrousov Tags: Epidemiology Source Type: research
Trends in the discovery of new drugs for Mycobacterium tuberculosis therapy with a glance at resistance
Despite the low expensive and effective four-drug treatment regimen (isoniazid, rifampicin, pyrazinamide and ethambutol) was introduced 40 years ago, TB continues to cause considerable morbidity and mortality worldwide. In 2015, the WHO estimated a total of 10.4 million new tuberculosis (TB) cases worldwide. Currently, the increased number of multidrug-resistant (MDR-TB), extensively-drug resistant (XDR-TB) and in some recent reports, totally drug-resistant TB (TDR-TB) cases raises concerns about this disease. (Source: Tuberculosis)
Source: Tuberculosis - December 8, 2017 Category: Respiratory Medicine Authors: Vahid Lohrasbi, Malihe Talebi, Abed Zahedi Bialvaei, Lanfranco Fattorini, Michel Drancourt, Mohsen Heidary, Davood Darban-Sarokhalil Tags: Review Source Type: research
Trends in the discovery of new drugs for Mycobacterium tuberculosis therapy with a glance at resistance
Despite the introduction 40 years ago of the low expensive and effective four-drug (isoniazid, rifampicin, pyrazinamide and ethambutol) treatment regimen, TB continues to cause considerable morbidity and mortality worldwide. in 2015, WHO estimated a total of 10.4 million new tuberculosis (TB) cases worldwide. Currently, the increased number of multidrug-resistant (MDR-TB), extensively-drug resistant (XDR-TB) and in some recent reports totally drug-resistant TB (TDR-TB) cases raises concerns about this disease. (Source: Tuberculosis)
Source: Tuberculosis - December 8, 2017 Category: Respiratory Medicine Authors: Vahid Lohrasbi, Malihe Talebi, Abed Zahedi Bialvaei, Lanfranco Fattorini, Michel Drancourt, Mohsen Heidary, Davood Darban-Sarokhalil Tags: Review Source Type: research
M. bovis infection in pigs: Improvement of the γ-IFN assay efficiency in this species using a maintenance medium
This study set up a defined broth culture in which lymphocytes, the cell population predominantly responsible for IFN-γ production, are maintained in a s teady-state and their vitality is preserved. (Source: Tuberculosis)
Source: Tuberculosis - December 1, 2017 Category: Respiratory Medicine Authors: Elisabetta Gerace, Francesca Mandanici, Paolo Pasquali, Maria Falduto, Maria Vitale, Vincenzo Di Marco Lo Presti, Benedetta Amato Source Type: research
Activity loss by H46A mutation in Mycobacterium tuberculosis isocitrate lyase is due to decrease in structural plasticity and collective motions of the active site
Mycobacterium tuberculosis isocitrate lyase (MtbICL) is a crucial enzyme of the glyoxylate cycle and is a validated anti-tuberculosis drug target. Structurally distant, non-active site mutation (H46A) in MtbICL has been found to cause loss of enzyme activity. The aim of the present work was to explore the structural alterations induced by H46A mutation that caused the loss of enzyme activity. The structural and dynamic consequences of H46A mutation were studied using multiple computational methods such as docking, molecular dynamics simulation and residue interaction network analysis (RIN). (Source: Tuberculosis)
Source: Tuberculosis - November 29, 2017 Category: Respiratory Medicine Authors: Rohit Shukla, Harish Shukla, Timir Tripathi Source Type: research
