Normalised quantitative polymerase chain reaction for diagnosis of tuberculosis-associated uveitis

In this study, we investigated normalised quantitative PCR (nqPCR) in ocular fluids (aqueous/vitreous) for diagnosis of TBU in a TB-endemic population. Mycobacterial copy numbers (mpb64 gene) were normalised to host genome copy numbers (RNAse P RNA component H1 [RPPH1] gene) in TBU (n  = 16) and control (n = 13) samples (discovery cohort).

Source: Tuberculosis - March 26, 2018 Category: Respiratory Medicine Authors: Manas Ranjan Barik, Soveeta Rath, Rohit Modi, Rajkishori Rana, Mamatha M. Reddy, Soumyava Basu Source Type: research