C5aR contributes to the weak Th1 profile induced by an outbreak strain of Mycobacterium tuberculosis

C5a anaphylatoxin is a component of the complement system involved in the modulation of T-cell polarization. Herein we investigated whether C5a receptors, C5aR and C5L2, modulate the cytokine profiles induced by Mycobacterium tuberculosis (Mtb). We analyzed the impact of both receptors on T helper cell polarization induced by the multidrug resistant outbreak strain named M, which is a poor IFN- γ inducer compared with the laboratory strain H37Rv. To this aim, we first blocked C5aR or C5L2 of peripheral blood monocytes (Mo) from patients with tuberculosis and healthy donors, then we stimulated the Mo either with H37Rv or the M strain, and finally we analyzed cytokine profiles of Mo/macroph ages (MΦ) and CD4+ T-cells.

Source: Tuberculosis - December 20, 2016 Category: Respiratory Medicine Authors: Carmen Alejandra Sabio y Garc ía, Noemí Yokobori, Juan Ignacio Basile, Luciana Balboa, Alejandra González, Beatriz López, Viviana Ritacco, Silvia de la Barrera, María del Carmen Sasiain Source Type: research