Upregulation of cystathionine-ß-synthetase expression contributes to inflammatory pain in rat temporomandibular joint
Conclusion:
These data together with our previous report indicate that endogenous H2S generating enzyme CBS plays an important role in TMJ inflammation, which is likely mediated by inhibition of IK currents, thus identifying a specific molecular mechanism underlying pain and sensitization in TMJ inflammation. (Source: Molecular Pain)
Source: Molecular Pain - February 3, 2014 Category: Molecular Biology Authors: Xiuhua MiaoXiaowen MengGeping WuZhong JuHong-Hong ZhangShufen HuGuang-Yin Xu Source Type: research
Upregulation of cystathionine-beta-synthetase expression contributes to inflammatory pain in rat temporomandibular joint
Conclusion:
These data together with our previous report indicate that endogenous H2S generating enzyme CBS plays an important role in TMJ inflammation, which is likely mediated by inhibition of IK currents, thus identifying a specific molecular mechanism underlying pain and sensitization in TMJ inflammation. (Source: Molecular Pain)
Source: Molecular Pain - February 3, 2014 Category: Molecular Biology Authors: Xiuhua MiaoXiaowen MengGeping WuZhong JuHong-Hong ZhangShufen HuGuang-Yin Xu Source Type: research
A comparison of RNA-seq and exon arrays for whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat
Conclusion:
We recommend the use of RNA-seq for future high-throughput transcriptomic experiments in pain studies. RNA-seq allowed the identification of a larger number of putative candidate pain genes than microarrays and can also detect a wider range of expression values in a neuropathic pain model. In addition, RNA-seq can interrogate the whole genome regardless of prior annotations, being able to detect transcription from areas of the genome not currently annotated as exons. Some of these areas are differentially expressed following nerve injury, and may represent novel genes or isoforms. We also recommend the use of a...
Source: Molecular Pain - January 28, 2014 Category: Molecular Biology Authors: James PerkinsAna Antunes-MartinsMargarita CalvoJohn GristWerner RustRamona SchmidTobias HildebrandtMatthias KohlChristine OrengoStephen McMahonDavid Bennett Source Type: research
Anoctamin 1 contributes to inflammatory and nerve-injury induced hypersensitivity
Conclusions:
In addition to ANO's role in mediating acute thermal pain as a heat sensor, ANO1 is also capable of augmenting the excitability of DRG neurons under inflammatory or neuropathic conditions and thereby aggravates inflammation- or tissue injury-induced pathological pain. (Source: Molecular Pain)
Source: Molecular Pain - January 23, 2014 Category: Molecular Biology Authors: Byeongjun LeeHawon ChoJooyoung JungYoung Duk YangDong-Jin YangUhtaek Oh Source Type: research
A putative relay circuit providing low-threshold mechanoreceptive input to lamina I projection neurons via vertical cells in lamina II of the rat dorsal horn
Conclusions:
These results show that vertical cell dendritic spines are frequently contacted by the central terminals of myelinated low-threshold mechanoreceptive afferents. Since dendritic spines are associated with excitatory synapses, it is likely that most of these contacts were synaptic. Vertical cells in lamina II are therefore a potential route through which tactile afferents can activate lamina I projection neurons, and this pathway could play a role in tactile allodynia. (Source: Molecular Pain)
Source: Molecular Pain - January 17, 2014 Category: Molecular Biology Authors: Toshiharu YasakaSheena TiongErika PolgárMasahiko WatanabeEiichi KumamotoJohn RiddellAndrew Todd Source Type: research
Acid evoked thermal hyperalgesia involves peripheral P2Y1 receptor mediated TRPV1 phosphorylation in a rodent model of thrombus induced ischemic pain
Conclusion:
Collectively these data show that maintenance of an acidic environment in the ischemic hind paw of TIIP rats results in the phosphorylation of TRPV1 receptors via a PKC-dependent pathway, which leads to the development of TH mimicking what occurs in chronic ischemic patients with severe acidosis. More importantly, peripheral P2Y1 receptors play a pivotal role in this process, suggesting a novel peripheral mechanism underlying the development of TH in these patients. (Source: Molecular Pain)
Source: Molecular Pain - January 9, 2014 Category: Molecular Biology Authors: Soon-Gu KwonDae-Hyun RohSeo-Yeon YoonJi-Young MoonSheu-Ran ChoiHoon-Seong ChoiSuk-Yun KangHo-Jae HanAlvin BeitzSeog OhJang-Hern Lee Source Type: research
Loss of long-term depression in the insular cortex after tail amputation in adult mice
In this study, we investigate injury-related metaplastic changes in insular synaptic plasticity after distal tail amputation. We found that tail amputation in adult mice produced a selective loss of low frequency stimulation-induced LTD in the IC, without affecting (RS)-3,5-dihydroxyphenylglycine (DHPG)-evoked LTD. The impaired insular LTD could be pharmacologically rescued by priming the IC slices with a lower dose of DHPG application, a form of metaplasticity which involves activation of protein kinase C but not protein kinase A or calcium/calmodulin-dependent protein kinase II. These findings provide important insights ...
Source: Molecular Pain - January 8, 2014 Category: Molecular Biology Authors: Ming-Gang LiuMin Zhuo Source Type: research
Spinal morphine but not ziconotide or gabapentin analgesia is affected by alternative splicing of voltage-gated calcium channel CaV2.2 pre-mRNA
Presynaptic voltage-gated calcium CaV2.2 channels play a privileged role in spinal level sensitization following peripheral nerve injury. Direct and indirect inhibitors of CaV2.2 channel activity in spinal dorsal horn are analgesic in chronic pain states. CaV2.2 channels represent a family of splice isoforms that are expressed in different combinations according to cell-type. A pair of mutually exclusive exons in the CaV2.2 encoding Cacna1b gene, e37a and e37b, differentially influence morphine analgesia. In mice that lack exon e37a, which is enriched in nociceptors, the analgesic efficacy of intrathecal morphine against n...
Source: Molecular Pain - December 26, 2013 Category: Molecular Biology Authors: Yu-Qiu JiangArturo AndradeDiane Lipscombe Source Type: research
Activation of cyclin-dependent kinase 5 mediates orofacial mechanical hyperalgesia
Conclusions:
Collectively, our findings demonstrate for the first time the important role of Cdk5 in orofacial mechanical nociception. Modulation of Cdk5 activity in primary sensory neurons makes it an attractive potential target for the development of novel analgesics that could be used to treat multiple orofacial pain conditions. (Source: Molecular Pain)
Source: Molecular Pain - December 21, 2013 Category: Molecular Biology Authors: Michaela ProchazkovaAnita TerseNiranjana AminBradford HallElias UtrerasHarish PantAshok Kulkarni Source Type: research
Intrathecal leptin inhibits expression of the P2X2/3 receptors and alleviates neuropathic pain induced by chronic constriction sciatic nerve injury
Conclusions:
Our findings suggest that exogenous leptin can alleviate the chronic neuropathic pain caused by CCI. The leptin effect may be mediated by attenuated expression of IL-6, TNFalpha, and the P2X2 and P2X3 receptors in the DRG of CCI rats. (Source: Molecular Pain)
Source: Molecular Pain - December 10, 2013 Category: Molecular Biology Authors: Xin LiLumei KangGuilin LiHuihong ZengLei ZhangXiang LingHui DongShangdong LiangHongping Chen Source Type: research
Analgesic tolerance of opioid agonists in mutant mu-opioid receptors expressed in sensory neurons following intrathecal plasmid gene delivery
Conclusions:
These results indicate that phosphorylation of T394 plays a critical role in determining the potency of DAMGO-induced analgesia and IK desensitization, but has limited effect on morphine-induced responses. On the other hand, the mutation contributes minimally to both DAMGO- and morphine-induced behavioral tolerance. Furthermore, the study shows that plasmid gene delivery of mutant receptors to DRG neurons is a useful strategy to explore nociceptive behavioral consequences of the mutation. (Source: Molecular Pain)
Source: Molecular Pain - December 5, 2013 Category: Molecular Biology Authors: Guangwen LiFei MaYanping GuLi-Yen Huang Source Type: research
Mutated CaV2.1 channels dysregulate CASK/P2X3 signaling in mouse trigeminal sensory neurons of R192Q Cacna1a knock-in mice
Conclusions:
We propose that, in trigeminal sensory neurons, the CASK/P2X3 complex has a dynamic nature depending on intracellular calcium and related signaling, that are enhanced in a transgenic mouse model of genetic hemiplegic migraine. (Source: Molecular Pain)
Source: Molecular Pain - December 2, 2013 Category: Molecular Biology Authors: Aswini GnanasekaranTanja BeleSwathi HullugundiManuela SimonettiMichael FerrariArn van den MaagdenbergAndrea NistriElsa Fabbretti Source Type: research
Mechanical sensitization of cutaneous sensory fibers in the spared nerve injury mouse model
Conclusions:
To our knowledge, this is the first study evaluating the contribution of primary afferent fibers in the SNI model. These data suggest that enhanced suprathreshold firing in AM and C fibers may play a role in the marked, persistent mechanical hypersensitivity observed in this model. These results may provide insight into mechanisms underlying neuropathic pain in humans. (Source: Molecular Pain)
Source: Molecular Pain - November 29, 2013 Category: Molecular Biology Authors: Amanda SmithCrystal O¿HaraCheryl Stucky Source Type: research
Inhibition of tetrodotoxin-resistant sodium current in dorsal root ganglia neurons mediated by D1/D5 dopamine receptors
Background:
Dopaminergic fibers originating from area A11 of the hypothalamus project to different levels of the spinal cord and represent the major source of dopamine. In addition, tyrosine hydroxylase, the rate-limiting enzyme for the synthesis of catecholamines, is expressed in 8-10% of dorsal root ganglia (DRG) neurons, suggesting that dopamine may be released in the dorsal root ganglia. Dopamine has been shown to modulate calcium current in DRG neurons, but the effects of dopamine on sodium currents and on the firing properties of small DRG neurons are poorly understood.
Results:
The effects of dopamine and dopamine r...
Source: Molecular Pain - November 28, 2013 Category: Molecular Biology Authors: William GalbavyElham SafaieMario RebecchiMichelino Puopolo Source Type: research
Correction: Inhibition of endogenous NGF degradation induces mechanical allodynia and thermal hyperalgesia in rats
No description available (Source: Molecular Pain)
Source: Molecular Pain - November 26, 2013 Category: Molecular Biology Authors: Maria OsikowiczGeraldine LongoSimon AllardA Claudio CuelloAlfredo Ribeiro-da-Silva Source Type: research
