Small-conductance calcium-activated potassium (SK) channels in the amygdala mediate pain-inhibiting effects of clinically available riluzole in a rat model of arthritis pain
Conclusions:
The results suggest that riluzole can inhibit supraspinally organized pain behaviors in an arthritis model by activating SK, but not BK, channels in the amygdala (CeA but not BLA). (Source: Molecular Pain)
Source: Molecular Pain - August 28, 2015 Category: Molecular Biology Authors: Jeremy ThompsonGuangchen JiVolker Neugebauer Source Type: research
Fatty acid binding protein deletion suppresses inflammatory pain through endocannabinoid/N-acylethanolamine-dependent mechanisms
Conclusions:
This study demonstrates that mice lacking FABPs possess elevated levels of N-acylethanolamines, consistent with the idea that FABPs regulate the endocannabinoid and N-acylethanolamine tone in vivo. The antinociceptive effects observed in the knockout mice support a role for FABPs in regulating nociception and suggest that these proteins should serve as targets for the development of future analgesics. (Source: Molecular Pain)
Source: Molecular Pain - August 28, 2015 Category: Molecular Biology Authors: Martin KaczochaSherrye GlaserThomas MaherBrendan ClavinJohn HamiltonJoseph O¿RourkeMario RebecchiMichelino PuopoloYuji OwadaPanayotis Thanos Source Type: research
Recruitment of dorsal midbrain catecholaminergic pathways in the recovery from nerve injury evoked disabilities
Conclusions:
Our data provide evidence for: (1) potential increases in dopamine and noradrenaline synthesis in vPAG cells; and (2) increased catecholaminergic drive on vPAG neurons in rats in which transient changes in social behavior are seen following CCI. The data suggests a role for dopaminergic and noradrenergic outputs, and catecholaminergic inputs of the vPAG in the expression of one of the profiles of behavioural and endocrine change triggered by nerve injury. (Source: Molecular Pain)
Source: Molecular Pain - August 18, 2015 Category: Molecular Biology Authors: David MorJames KangPeter WyllieVignaraja ThirunavukarasuHayden HoultonPaul AustinKevin Keay Source Type: research
Short-term anesthesia inhibits formalin-induced extracellular signal-regulated kinase (ERK) activation in the rostral anterior cingulate cortex but not in the spinal cord
In this study, we investigated whether administration of anesthesia at the time of injury could reduce phosphorylated-ERK (PERK) expression in the rACC, which might eliminate the negative affective component of noxious stimulation. Intraplantar hindpaw formalin stimulation, an aversive event in the awake animal, was given with or without general isoflurane anesthesia, and PERK expression was subsequently quantified in the rACC using immunohistochemistry. Furthermore, as numerous studies have demonstrated the importance of spinal ERK signaling in the regulation of nociceptive behaviour, we also examined PERK in the superfic...
Source: Molecular Pain - August 14, 2015 Category: Molecular Biology Authors: Keri TochikiMaria MaiarùJames MillerStephen HuntSandrine Géranton Source Type: research
p38 phosphorylation in medullary microglia mediates ectopic orofacial inflammatory pain in rats
Conclusions:
These findings indicate that microglia, activated via p38 phosphorylation, play a pivotal role in WDR neuronal hyperexcitability, which accounts for the mechanical hypersensitivity in the lateral facial skin associated with trapezius muscle inflammation. (Source: Molecular Pain)
Source: Molecular Pain - August 12, 2015 Category: Molecular Biology Authors: Masaaki KiyomotoMasamichi ShinodaKuniya HondaYuka NakayaKo DezawaAyano KatagiriSatoshi KamakuraTomio InoueKoichi Iwata Source Type: research
Amelioration of the reduced antinociceptive effect of morphine in the unpredictable chronic mild stress model mice by noradrenalin but not serotonin reuptake inhibitors
Conclusions:
We demonstrated that the antinociceptive effect of morphine but not tramadol was reduced in mice that had experienced UCMS. The reduced antinociceptive effect of morphine under the UCMS condition was ameliorated by pretreatment with maprotiline but not escitalopram. These results suggest that the reduced antinociceptive effects of morphine under conditions of chronic stress may be ameliorated by activation of the noradrenergic but not the serotonergic system. (Source: Molecular Pain)
Source: Molecular Pain - August 11, 2015 Category: Molecular Biology Authors: Soichiro IdeHiroshi SatoyoshiMasabumi MinamiMasamichi Satoh Source Type: research
Koumine enhances spinal cord 3α-hydroxysteroid oxidoreductase expression and activity in a rat model of neuropathic pain
Conclusion:
This study demonstrates that 3α-HSOR is an important molecular target of koumine for alleviating neuropathic pain. Koumine may prove a promising compound for the development of novel analgesic agents effective against intractable neuropathic pain. (Source: Molecular Pain)
Source: Molecular Pain - August 9, 2015 Category: Molecular Biology Authors: Hong-Qiang QiuYing XuGui-Lin JinJian YangMing LiuSu-Ping LiChang-Xi Yu Source Type: research
KCNQ channels in nociceptive cold-sensing trigeminal ganglion neurons as therapeutic targets for treating orofacial cold hyperalgesia
Conclusion:
Taken together, these findings indicate that KCNQ channel plays a significant role in controlling cold sensitivity and is a therapeutic target for alleviating trigeminal neuropathic pain that manifests orofacial cold hyperalgesia. (Source: Molecular Pain)
Source: Molecular Pain - July 31, 2015 Category: Molecular Biology Authors: Alaa Abd-ElsayedRyo IkedaZhanfeng JiaJennifer LingXiaozhuo ZuoMin LiJianguo Gu Source Type: research
Identification of lncRNA expression profile in the spinal cord of mice following spinal nerve ligation-induced neuropathic pain
Conclusion:
Our findings uncovered the expression pattern of lncRNAs and mRNAs in the mice spinal cord under neuropathic pain condition. These lncRNAs and mRNAs may represent new therapeutic targets for the treatment of neuropathic pain. (Source: Molecular Pain)
Source: Molecular Pain - July 17, 2015 Category: Molecular Biology Authors: Bao-Chun JiangWen-Xing SunLi-Na HeDe-Li CaoZhi-Jun ZhangYong-Jing Gao Source Type: research
Distribution of raphespinal fibers in the mouse spinal cord
Conclusion:
Serotonergic and non-serotonergic fibers arising from the hindbrain raphe and reticular nuclei had similar termination pattern in the mouse spinal cord with subtle difference. The present study provides anatomical foundation for the multiple roles raphe and adjacent reticular nuclei play. (Source: Molecular Pain)
Source: Molecular Pain - July 16, 2015 Category: Molecular Biology Authors: Huazheng LiangShaoshi WangRichard FrancisRenee WhanCharles WatsonGeorge Paxinos Source Type: research
Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats
Conclusions:
There is functional anoctamin-1 and bestrophin-1 expression in rats at sites related to nociceptive processing. Blockade of these CaCCs suppresses compound action potential generation in putative C fibers and lessens established tactile allodynia. As CaCCs activity contributes to neuropathic pain maintenance, selective inhibition of their activity may function as a tool to generate analgesia in nerve injury pain states. (Source: Molecular Pain)
Source: Molecular Pain - July 1, 2015 Category: Molecular Biology Authors: Jorge Pineda-FariasPaulino Barragán-IglesiasEmanuel Loeza-AlcocerJorge Torres-LópezHéctor Rocha-GonzálezFrancisca Pérez-SeverianoRodolfo Delgado-LezamaVinicio Granados-Soto Source Type: research
Temporal dynamics of anxiety phenotypes in a dental pulp injury model
Conclusions:
Together, these results suggest a temporally progressive causal relationship between orofacial pain and anxiety, calling for more in-depth mechanistic studies on concomitant pain and anxiety disorders. (Source: Molecular Pain)
Source: Molecular Pain - June 30, 2015 Category: Molecular Biology Authors: Lin ShangTian-Le XuFei LiJiansheng SuWei-Guang Li Source Type: research
Function and postnatal changes of dural afferent fibers expressing TRPM8 channels
Conclusions:
Our results indicate that the TRPM8-expressing dural afferent fibers undergo cell- and target tissue-specific axonal pruning during postnatal development. Activation of dural TRPM8 channels decreases meningeal irritation-evoked nocifensive behavior in adult mice. This provides a framework to further explore the role of postnatal changes of TRPM8-expressing dural afferents in the pathophysiology of pediatric and adult migraine. (Source: Molecular Pain)
Source: Molecular Pain - June 26, 2015 Category: Molecular Biology Authors: Lynn RenAjay DhakaYu-Qing Cao Source Type: research
Mechanisms underlying clinical efficacy of Angiotensin II type 2 receptor (AT 2 R) antagonist EMA401 in neuropathic pain: clinical tissue and in vitro studies
Background:
The clinical efficacy of the Angiotensin II (AngII) receptor AT
2
R antagonist EMA401, a novel peripherally-restricted analgesic, was reported recently in post-herpetic neuralgia. While previous studies have shown that AT
2
R is expressed by nociceptors in human DRG (hDRG), and that EMA401 inhibits capsaicin responses in cultured hDRG neurons, the expression and levels of its endogenous ligands AngII and AngIII in clinical neuropathic pain tissues, and their signalling pathways, require investigation. We have immunostained AngII, ...
Source: Molecular Pain - June 26, 2015 Category: Molecular Biology Authors: Uma AnandYiangos YiangouMarco SinisiMichael FoxAnthony MacQuillanTom QuickYuri KorchevChas BountraTom McCarthyPraveen Anand Source Type: research
Expression changes of microRNA-1 and its targets Connexin 43 and brain-derived neurotrophic factor in the peripheral nervous system of chronic neuropathic rats
Conclusions:
This study demonstrates that CCI leads to a regulation of miRNAs (miR-1) in the peripheral nervous system. This regulation is associated with alterations in the expression and localization of the miR-1 dependent pain-relevant proteins BDNF and Cx43. Further studies will have to explore the function of miRNAs in the context of neuropathic pain in the peripheral nervous system. (Source: Molecular Pain)
Source: Molecular Pain - June 26, 2015 Category: Molecular Biology Authors: Elena NeumannHenning HermannsFranziska BarthelRobert WerdehausenTimo Brandenburger Source Type: research
