Phospholipase C gamma mediates endogenous brain-derived neurotrophic factor-regulated calcitonin gene-related peptide expression in colitis-induced visceral pain
Conclusion
These results suggest that colitis-induced and brain-derived neurotrophic factor-mediated calcitonin gene-related peptide expression in sensory activation is regulated by a unique pathway involving brain-derived neurotrophic factor-phospholipase C gamma-cAMP-responsive element binding protein axis. (Source: Molecular Pain)
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Hashmi, F., Liu, M., Shen, S., Qiao, L.-Y. Tags: Research Article Source Type: research
NGF-trkA signaling modulates the analgesic effects of prostatic acid phosphatase in resiniferatoxin-induced neuropathy
Conclusions
This study revealed that functional PAP(+) neurons are essential for the analgesic effect, which is mediated by NGF-trkA signaling. (Source: Molecular Pain)
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Wu, C.-H., Ho, W.-Y., Lee, Y.-C., Lin, C.-L., Hsieh, Y.-L. Tags: Research Article Source Type: research
Oligodendrocytes in HIV-associated pain pathogenesis
Conclusion
These findings suggest that oligodendrocytes are reactive during the pathogenesis of HIV-associated pain. However, interfering with myelination does not alter the induction of gp120-induced pain. (Source: Molecular Pain)
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Shi, Y., Shu, J., Liang, Z., Yuan, S., Tang, S.-J. Tags: Research Article Source Type: research
Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
Conclusion
This is the first longitudinal study providing detailed insights into various voluntary behavioral parameters related to pain and highlights the importance of social environment on spontaneous non-evoked behaviors in a mouse model of chronic neuropathy. Our results provide fundamental considerations for future experimental planning and discussion of pain-related behavioral changes. (Source: Molecular Pain)
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Pitzer, C., Kuner, R., Tappe-Theodor, A. Tags: Research Article Source Type: research
Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development
Conclusion
Altogether, these results validate CCR2 as a an appropriate molecular target for pain control and demonstrate that RNAi-based gene therapy represent an highly specific alternative to classical pharmacological approaches to treat central pathologies such as chronic pain. (Source: Molecular Pain)
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Begin-Lavallee, V., Midavaine, E., Dansereau, M.-A., Tetreault, P., Longpre, J.-M., Jacobi, A. M., Rose, S. D., Behlke, M. A., Beaudet, N., Sarret, P. Tags: Research Article Source Type: research
Go-sha-jinki-Gan (GJG) ameliorates allodynia in chronic constriction injury model mice via suppression of TNF-{alpha} expression in the spinal cord
Conclusions
Our data suggest that GJG ameliorates allodynia in chronic constriction injury model mice via suppression of tumor necrosis factor-α expression derived from activated microglia. GJG is a promising drug for the treatment of neuropathic pain induced by neuro-inflammation. (Source: Molecular Pain)
Source: Molecular Pain - June 12, 2016 Category: Molecular Biology Authors: Nakanishi, M., Nakae, A., Kishida, Y., Baba, K., Sakashita, N., Shibata, M., Yoshikawa, H., Hagihara, K. Tags: Research Article Source Type: research
Intact subepidermal nerve fibers mediate mechanical hypersensitivity via the activation of protein kinase C gamma in spared nerve injury
Conclusions
From results obtained in this study, we strongly recommend that the intact SENFs persistently increase PKC activation and mTOR-dependent PKC synthesis participate in the initiation and maintenance of mechanical hypersensitivity in spared nerve injury, which represents as a novel insight into the therapeutic strategy of pain in the periphery. (Source: Molecular Pain)
Source: Molecular Pain - June 12, 2016 Category: Molecular Biology Authors: Ko, M.-H., Yang, M.-L., Youn, S.-C., Lan, C.-T., Tseng, T.-J. Tags: Research Article Source Type: research
Brain morphological alternation in chronic pain patients with neuropathic characteristics
Conclusions
Our findings suggest that neuropathic characteristics strongly affect the brain regions related to modulation of pain in patients with chronic pain and, therefore, contribute to the severity of chronic pain. (Source: Molecular Pain)
Source: Molecular Pain - June 7, 2016 Category: Molecular Biology Authors: Sugimine, S., Ogino, Y., Kawamichi, H., Obata, H., Saito, S. Tags: Research Article Source Type: research
Spinal neurons that contain gastrin-releasing peptide seldom express Fos or phosphorylate extracellular signal-regulated kinases in response to intradermal chloroquine
Conclusions
Both expression of Fos and phosphorylation of ERK can be used to identify dorsal horn neurons activated by chloroquine injection. However, these results do not support the hypothesis that interneurons expressing GRP are critical components in the itch pathway. (Source: Molecular Pain)
Source: Molecular Pain - June 6, 2016 Category: Molecular Biology Authors: Bell, A. M., Gutierrez-Mecinas, M., Polgar, E., Todd, A. J. Tags: Research Article Source Type: research
Cingulate GABA levels inversely correlate with the intensity of ongoing chronic knee osteoarthritis pain
Conclusion
Our study supports mid-anterior cingulate cortex -aminobutyric acid as a potential marker of pain severity in chronic nociceptive pain states independent of negative affect. The findings suggest that GABAergic disinhibition of the salience network may underlie sensitization to averse stimuli as a mechanism contributing to pain chronification. (Source: Molecular Pain)
Source: Molecular Pain - May 19, 2016 Category: Molecular Biology Authors: Reckziegel, D., Raschke, F., Cottam, W. J., Auer, D. P. Tags: Research Article Source Type: research
Cannabinoid 1 receptor knockout mice display cold allodynia, but enhanced recovery from spared-nerve injury-induced mechanical hypersensitivity
Conclusion
Cold allodynia and significant recovery from spared-nerve injury-induced mechanical hypersensitivity are two novel phenotypes which characterize the global CB1R–/– mice. An increase in transient receptor potential channel of melastatin 8 channel function in DRG neurons may underlie the cold phenotype. Recovery of mechanical thresholds in the CB1R knockouts was independent of motor function. These results indicate that CB1R expression contributes to the development of persistent mechanical hypersensitivity, protects against the development of robust cold allodynia but is not involved in motor impairme...
Source: Molecular Pain - May 19, 2016 Category: Molecular Biology Authors: Sideris, A., Piskoun, B., Russo, L., Norcini, M., Blanck, T., Recio-Pinto, E. Tags: Research Article Source Type: research
Downregulation of Cdh1 signalling in spinal dorsal horn contributes to the maintenance of mechanical allodynia after nerve injury in rats
Conclusions
This study indicates that a downregulation of Cdh1 expression in spinal dorsal horn is involved in molecular mechanisms underlying the maintenance of neuropathic pain. Upregulation of spinal Cdh1 may be a promising approach to treat neuropathic pain. (Source: Molecular Pain)
Source: Molecular Pain - May 15, 2016 Category: Molecular Biology Authors: Hu, R., Li, L., Li, D., Tan, W., Wan, L., Zhu, C., Zhang, Y., Zhang, C., Yao, W. Tags: Research Article Source Type: research
A pro-nociceptive phenotype unmasked in mice lacking fatty-acid amide hydrolase
Fatty-acid amide hydrolase (FAAH) is the major enzyme responsible for degradation of anandamide, an endocannabinoid. Pharmacological inhibition or genetic deletion of FAAH (FAAH KO) produces antinociception in preclinical pain models that is largely attributed to anandamide-induced activation of cannabinoid receptors. However, FAAH metabolizes a wide range of structurally related, biologically active lipid signaling molecules whose functions remain largely unknown. Some of these endogenous lipids, including anandamide itself, may exert pro-nociceptive effects under certain conditions. In our study, FAAH KO mice exhibited a...
Source: Molecular Pain - May 12, 2016 Category: Molecular Biology Authors: Carey, L. M., Slivicki, R. A., Leishman, E., Cornett, B., Mackie, K., Bradshaw, H., Hohmann, A. G. Tags: Research Article Source Type: research
Pre-LTP requires extracellular signal-regulated kinase in the ACC
In this study, we examined whether pre-long-term potentiation in the anterior cingulate cortex requires the activation of presynaptic extracellular signal-regulated kinase. We found that p42/p44 mitogen-activated protein kinase inhibitors, PD98059 and U0126, suppressed the induction of pre-long-term potentiation. By contrast, these inhibitors did not affect the maintenance of pre-long-term potentiation. Using pharmacological inhibitors, we found that pre-long-term potentiation recorded for 1 h did not require transcriptional or translational processes. Our results strongly indicate that the activation of presynaptic extrac...
Source: Molecular Pain - May 12, 2016 Category: Molecular Biology Authors: Yamanaka, M., Tian, Z., Darvish-Ghane, S., Zhuo, M. Tags: Research Article Source Type: research
Diffuse traumatic brain injury induces prolonged immune dysregulation and potentiates hyperalgesia following a peripheral immune challenge
Conclusions
We conclude that traumatic brain injury increased the inflammatory pain associated with cutaneous inflammation by contributing to systemic immune dysregulation. Regulatory T cells are immune suppressors and failure of T cells to differentiate into regulatory T cells leads to unregulated cytokine production which may contribute to the potentiation of peripheral pain through the excitation of peripheral sensory neurons. In addition, regulatory T cells are identified as a potential target for therapeutic rebalancing of peripheral immune homeostasis to improve functional outcome and decrease the incidence of periph...
Source: Molecular Pain - May 12, 2016 Category: Molecular Biology Authors: Rowe, R. K., Ellis, G. I., Harrison, J. L., Bachstetter, A. D., Corder, G. F., Van Eldik, L. J., Taylor, B. K., Marti, F., Lifshitz, J. Tags: Research Article Source Type: research
