5-HT2CR blockade in the amygdala conveys analgesic efficacy to SSRIs in a rat model of arthritis pain
Conclusions:
The data suggest that 5-HT2CR in the amygdala, especially in the BLA, limits the effectiveness of SSRIs to inhibit pain-related emotional-affective behaviors. (Source: Molecular Pain)
Source: Molecular Pain - August 12, 2013 Category: Molecular Biology Authors: Stéphanie GrégoireVolker Neugebauer Source Type: research
NaV1.7: Stress-induced changes in immunoreactivity within magnocellular neurosecretory neurons of the supraoptic nucleus
Conclusions:
NaV1.7 is present within neurosecretory neurons of rat supraoptic nucleus, where the level of immunoreactivity is dynamic, increasing in response to osmotic stress. Whether NaV1.7 levels are up-regulated within the human hypothalamus in response to environmental factors or stress, and whether NaV1.7 plays a functional role in human hypothalamus, is not yet known. Until these questions are resolved, the present findings suggest the need for careful assessment of hypothalamic function in patients with NaV1.7 mutations, especially when subjected to stress, and for monitoring of hypothalamic function as NaV1.7 blo...
Source: Molecular Pain - August 8, 2013 Category: Molecular Biology Authors: Joel BlackJanneke HoeijmakersCatherina FaberIngemar MerkiesStephen Waxman Source Type: research
Acute and chronic phases of complex regional pain syndrome in mice are accompanied by distinct transcriptional changes in the spinal cord
Conclusions:
Our data provide a global look at the transcriptional changes in the spinal cord that accompany the acute and chronic phases of CRPS as modeled in mice. Furthermore, it follows up on one of the top-regulated genes coding for CcL2 and validates its role in regulating nociception in the fracture/cast model of CRPS. (Source: Molecular Pain)
Source: Molecular Pain - August 8, 2013 Category: Molecular Biology Authors: Joseph GallagherMaral TajerianTianzhi GuoXiaoyou ShiWenwu LiMing ZhengGary PeltzWade KingeryJ Clark Source Type: research
Inhibition of endogenous NGF degradation induces mechanical allodynia and thermal hyperalgesia in rats
Conclusions:
These findings indicate that localized MMP-2/9 inhibition provokes a pattern of sensitization and fiber sprouting comparable to that previously obtained following CFA injection. Accordingly, the modulation of endogenous NGF levels should be considered as a potential therapeutic target for the management of inflammatory pain associated with arthritis. (Source: Molecular Pain)
Source: Molecular Pain - July 29, 2013 Category: Molecular Biology Authors: Maria OsikowiczGeraldine LongoSimon AllardA CuelloAlfredo Ribeiro-da-Silva Source Type: research
Intraperitoneal administration of AAV9-shRNA inhibits target gene expression in the dorsal root ganglia of neonatal mice
Conclusions:
Although we previously showed substantial inhibition of target gene expression in DRG via intrathecal ssAAV9-shRNA administration, here we succeeded in inhibiting target gene expression in DRG neurons via intraperitoneal injection of ssAAV9-shRNA. AAV9-mediated delivery of shRNA will pave the way for creating animal models for investigating the molecular biology of the mechanisms of pain and sensory ganglionopathies. (Source: Molecular Pain)
Source: Molecular Pain - July 18, 2013 Category: Molecular Biology Authors: Akira MachidaHiroya KuwaharaAzat MayraTakayuki KuboderaTakashi HiraiFumiko SunagaMio TajiriYukihiko HiraiTakashi ShimadaHidehiro MizusawaTakanori Yokota Source Type: research
PKCgamma receptor mediates visceral nociception and hyperalgesia following exposure to PTSD-like stress in the spinal cord of rats
Conclusions:
The modified SPS alters visceral sensitivity to CRD, and contributes to the maintenance of visceral hyperalgesia, which is associated with enhanced PKCgamma expression in the spinal cord. Functional blockade of the PKCgamma receptors attenuates SPS-induced visceral hyperalgesia. Thus, the present study identifies a specific molecular mechanism for visceral hyperalgesia which may pave the way for novel therapeutic strategies for PTSD-like conditions. (Source: Molecular Pain)
Source: Molecular Pain - July 9, 2013 Category: Molecular Biology Authors: Yu-Qin HeQiang ChenLei JiZheng-Guo WangZhi-Hong BaiRobert StephensMin Yang Source Type: research
Genome-wide association study of sensory disturbances in the inferior alveolar nerve after bilateral sagittal split ramus osteotomy
Conclusions:
The GWAS of sensory disturbances after BSSRO revealed associations between genetic polymorphisms located in the flanking region of the ARID1B and ZPLD1 genes and hypoesthesia and between a nonsynonymous genetic polymorphism in the METTL4 gene and dysesthesia. (Source: Molecular Pain)
Source: Molecular Pain - July 8, 2013 Category: Molecular Biology Authors: Daisuke KobayashiDaisuke NishizawaYoshito TakasakiShinya KasaiTakashi KakizawaKazutaka IkedaKen-ichi Fukuda Source Type: research
Shaped magnetic field pulses by multi-coil repetitive transcranial magnetic stimulation (rTMS) differentially modulate anterior cingulate cortex responses and pain in volunteers and fibromyalgia patients
Conclusion:
Multi-coil rTMS may be a safe and effective treatment option for acute as well as for chronic pain, such as that accompanying fibromyalgia. Further studies are necessary to optimize configurations and settings as well as to elucidate the mechanisms that lead to the long-lasting pain control produced by these treatments. (Source: Molecular Pain)
Source: Molecular Pain - July 2, 2013 Category: Molecular Biology Authors: Alexander TzabazisCarina ApariciMichael RowbothamM SchneiderAmit EtkinDavid Yeomans Source Type: research
Analgesic effect of a mixed T-type channel inhibitor/CB2 receptor agonist
Conclusions:
Our work shows that both T-type channels as well as CB2 receptors play a role in the antinociceptive action of NMP-181, and also provides a novel avenue for suppressing chronic pain through novel mixed T-type/cannabinoid receptor ligands. (Source: Molecular Pain)
Source: Molecular Pain - July 1, 2013 Category: Molecular Biology Authors: Vinicius GadottiHaitao YouRavil PetrovN BergerPhilippe DiazGerald Zamponi Source Type: research
Functionally important amino acid residues in the transient receptor potential vanilloid 1 (TRPV1) ion channel ¿ an overview of the current mutational data
This review aims to create an overview of the currently available results of site-directed mutagenesis studies on transient receptor potential vanilloid type 1 (TRPV1) receptor. Systematization of the vast number of data on the functionally important amino acid mutations of TRPV1 may provide a clearer picture of this field, and may promote a better understanding of the relationship between the structure and function of TRPV1. The review summarizes information on 112 unique mutated sites along the TRPV1, exchanged to multiple different residues in many cases. These mutations influence the effect or binding of different agon...
Source: Molecular Pain - June 22, 2013 Category: Molecular Biology Authors: Zoltán WinterAndrea BuhalaFerenc ÖtvösKatalin JósvayCsaba VizlerGyörgy DombiGerda SzakonyiZoltán Oláh Source Type: research
Functionally important amino acid residues in the transient receptor potential vanilloid 1 (TRPV1) ion channel -- an overview of the current mutational data
This review aims to create an overview of the currently available results of site-directed mutagenesis studies on transient receptor potential vanilloid type 1 (TRPV1) receptor. Systematization of the vast number of data on the functionally important amino acid mutations of TRPV1 may provide a clearer picture of this field, and may promote a better understanding of the relationship between the structure and function of TRPV1. The review summarizes information on 112 unique mutated sites along the TRPV1, exchanged to multiple different residues in many cases. These mutations influence the effect or binding of different agon...
Source: Molecular Pain - June 22, 2013 Category: Molecular Biology Authors: Zoltán WinterAndrea BuhalaFerenc ÖtvösKatalin JósvayCsaba VizlerGyörgy DombiGerda SzakonyiZoltán Oláh Source Type: research
Effect of amitriptyline on tetrodotoxin-resistant Nav1.9 currents in nociceptive trigeminal neurons
Conclusion:
These data suggest that AMI is a state-selective blocker of Nav1.9 channels in rat nociceptive trigeminal neurons, which likely contributes to the efficacy of AMI in treating various pains, including migraines. (Source: Molecular Pain)
Source: Molecular Pain - June 22, 2013 Category: Molecular Biology Authors: Jingyao LiangXiaoyan LiuJianquan ZhengShengyuan Yu Source Type: research
An LPA species (18:1 LPA) plays key roles in the self-amplification of spinal LPA production in the peripheral neuropathic pain model
Conclusion:
These results suggest that 18:1 LPA is the predominant ligand responsible for LPA1 and LPA3 receptors-mediated amplification of LPA production through microglial activation. (Source: Molecular Pain)
Source: Molecular Pain - June 17, 2013 Category: Molecular Biology Authors: Lin MaJun NagaiJerold ChunHiroshi Ueda Source Type: research
Operant behavioral responses to orofacial cold stimuli in rats with chronic constrictive trigeminal nerve injury: effects of menthol and capsazepine
Both spinal and trigeminal somatosensory systems use the TRPM8 channel as a principal transducer for detecting cold stimuli. It is currently unclear whether this cold transducer may play a role in trigeminal neuropathic pain manifesting cold allodynia and hyperalgesia. In the present study, trigeminal neuropathy was induced by chronic constrictive nerve injury of the infraorbital nerve (ION-CCI). Behavioral responses to cold stimuli in orofacial regions were assessed by the newly developed orofacial operant test in the ION-CCI rats. We tested menthol and capsazepine, two compounds that can activate and inhibit TRPM8 respec...
Source: Molecular Pain - June 14, 2013 Category: Molecular Biology Authors: Xiaozhuo ZuoJennifer LingGuang-Yin XuJianguo Gu Source Type: research
Subunit and frequency-dependent inhibition of Acid Sensing Ion Channels by local anesthetic tetracaine
Conclusions:
These findings disclose a potential new mechanism underlying the analgesic effects of local anesthetics, particularly in acidic conditions where their primary target (i.e. voltage-gated Na+ channel) has been suppressed by protons. (Source: Molecular Pain)
Source: Molecular Pain - June 10, 2013 Category: Molecular Biology Authors: Tiandong LengJun LinJames CottrellZhi-Gang Xiong Source Type: research
