Intraperitoneal administration of AAV9-shRNA inhibits target gene expression in the dorsal root ganglia of neonatal mice

Conclusions:
Although we previously showed substantial inhibition of target gene expression in DRG via intrathecal ssAAV9-shRNA administration, here we succeeded in inhibiting target gene expression in DRG neurons via intraperitoneal injection of ssAAV9-shRNA. AAV9-mediated delivery of shRNA will pave the way for creating animal models for investigating the molecular biology of the mechanisms of pain and sensory ganglionopathies.

Source: Molecular Pain - July 18, 2013 Category: Molecular Biology Authors: Akira MachidaHiroya KuwaharaAzat MayraTakayuki KuboderaTakashi HiraiFumiko SunagaMio TajiriYukihiko HiraiTakashi ShimadaHidehiro MizusawaTakanori Yokota Source Type: research